Job ID = 6456521 SRX = SRX3630321 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:55:46 prefetch.2.10.7: 1) Downloading 'SRR6652843'... 2020-06-21T10:55:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:05:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:05:37 prefetch.2.10.7: 1) 'SRR6652843' was downloaded successfully 2020-06-21T11:05:37 prefetch.2.10.7: 'SRR6652843' has 0 unresolved dependencies Read 66058099 spots for SRR6652843/SRR6652843.sra Written 66058099 spots for SRR6652843/SRR6652843.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:00 66058099 reads; of these: 66058099 (100.00%) were unpaired; of these: 3358553 (5.08%) aligned 0 times 50370633 (76.25%) aligned exactly 1 time 12328913 (18.66%) aligned >1 times 94.92% overall alignment rate Time searching: 00:18:00 Overall time: 00:18:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 11868749 / 62699546 = 0.1893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:41:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:41:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:41:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:41:10: 1000000 INFO @ Sun, 21 Jun 2020 20:41:15: 2000000 INFO @ Sun, 21 Jun 2020 20:41:19: 3000000 INFO @ Sun, 21 Jun 2020 20:41:24: 4000000 INFO @ Sun, 21 Jun 2020 20:41:28: 5000000 INFO @ Sun, 21 Jun 2020 20:41:33: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:41:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:41:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:41:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:41:38: 7000000 INFO @ Sun, 21 Jun 2020 20:41:40: 1000000 INFO @ Sun, 21 Jun 2020 20:41:42: 8000000 INFO @ Sun, 21 Jun 2020 20:41:45: 2000000 INFO @ Sun, 21 Jun 2020 20:41:47: 9000000 INFO @ Sun, 21 Jun 2020 20:41:50: 3000000 INFO @ Sun, 21 Jun 2020 20:41:52: 10000000 INFO @ Sun, 21 Jun 2020 20:41:55: 4000000 INFO @ Sun, 21 Jun 2020 20:41:56: 11000000 INFO @ Sun, 21 Jun 2020 20:41:59: 5000000 INFO @ Sun, 21 Jun 2020 20:42:01: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:42:04: 6000000 INFO @ Sun, 21 Jun 2020 20:42:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:42:06: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:42:06: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:42:06: 13000000 INFO @ Sun, 21 Jun 2020 20:42:09: 7000000 INFO @ Sun, 21 Jun 2020 20:42:10: 1000000 INFO @ Sun, 21 Jun 2020 20:42:11: 14000000 INFO @ Sun, 21 Jun 2020 20:42:13: 8000000 INFO @ Sun, 21 Jun 2020 20:42:15: 15000000 INFO @ Sun, 21 Jun 2020 20:42:15: 2000000 INFO @ Sun, 21 Jun 2020 20:42:18: 9000000 INFO @ Sun, 21 Jun 2020 20:42:20: 16000000 INFO @ Sun, 21 Jun 2020 20:42:20: 3000000 INFO @ Sun, 21 Jun 2020 20:42:23: 10000000 INFO @ Sun, 21 Jun 2020 20:42:25: 17000000 INFO @ Sun, 21 Jun 2020 20:42:25: 4000000 INFO @ Sun, 21 Jun 2020 20:42:27: 11000000 INFO @ Sun, 21 Jun 2020 20:42:29: 18000000 INFO @ Sun, 21 Jun 2020 20:42:30: 5000000 INFO @ Sun, 21 Jun 2020 20:42:32: 12000000 INFO @ Sun, 21 Jun 2020 20:42:34: 19000000 INFO @ Sun, 21 Jun 2020 20:42:35: 6000000 INFO @ Sun, 21 Jun 2020 20:42:37: 13000000 INFO @ Sun, 21 Jun 2020 20:42:39: 20000000 INFO @ Sun, 21 Jun 2020 20:42:39: 7000000 INFO @ Sun, 21 Jun 2020 20:42:42: 14000000 INFO @ Sun, 21 Jun 2020 20:42:44: 21000000 INFO @ Sun, 21 Jun 2020 20:42:44: 8000000 INFO @ Sun, 21 Jun 2020 20:42:47: 15000000 INFO @ Sun, 21 Jun 2020 20:42:48: 22000000 INFO @ Sun, 21 Jun 2020 20:42:49: 9000000 INFO @ Sun, 21 Jun 2020 20:42:51: 16000000 INFO @ Sun, 21 Jun 2020 20:42:53: 23000000 INFO @ Sun, 21 Jun 2020 20:42:54: 10000000 INFO @ Sun, 21 Jun 2020 20:42:56: 17000000 INFO @ Sun, 21 Jun 2020 20:42:58: 24000000 INFO @ Sun, 21 Jun 2020 20:42:58: 11000000 INFO @ Sun, 21 Jun 2020 20:43:01: 18000000 INFO @ Sun, 21 Jun 2020 20:43:02: 25000000 INFO @ Sun, 21 Jun 2020 20:43:03: 12000000 INFO @ Sun, 21 Jun 2020 20:43:05: 19000000 INFO @ Sun, 21 Jun 2020 20:43:07: 26000000 INFO @ Sun, 21 Jun 2020 20:43:08: 13000000 INFO @ Sun, 21 Jun 2020 20:43:10: 20000000 INFO @ Sun, 21 Jun 2020 20:43:12: 27000000 INFO @ Sun, 21 Jun 2020 20:43:13: 14000000 INFO @ Sun, 21 Jun 2020 20:43:15: 21000000 INFO @ Sun, 21 Jun 2020 20:43:17: 28000000 INFO @ Sun, 21 Jun 2020 20:43:18: 15000000 INFO @ Sun, 21 Jun 2020 20:43:20: 22000000 INFO @ Sun, 21 Jun 2020 20:43:21: 29000000 INFO @ Sun, 21 Jun 2020 20:43:22: 16000000 INFO @ Sun, 21 Jun 2020 20:43:24: 23000000 INFO @ Sun, 21 Jun 2020 20:43:26: 30000000 INFO @ Sun, 21 Jun 2020 20:43:27: 17000000 INFO @ Sun, 21 Jun 2020 20:43:29: 24000000 INFO @ Sun, 21 Jun 2020 20:43:31: 31000000 INFO @ Sun, 21 Jun 2020 20:43:32: 18000000 INFO @ Sun, 21 Jun 2020 20:43:34: 25000000 INFO @ Sun, 21 Jun 2020 20:43:35: 32000000 INFO @ Sun, 21 Jun 2020 20:43:37: 19000000 INFO @ Sun, 21 Jun 2020 20:43:39: 26000000 INFO @ Sun, 21 Jun 2020 20:43:40: 33000000 INFO @ Sun, 21 Jun 2020 20:43:42: 20000000 INFO @ Sun, 21 Jun 2020 20:43:43: 27000000 INFO @ Sun, 21 Jun 2020 20:43:45: 34000000 INFO @ Sun, 21 Jun 2020 20:43:47: 21000000 INFO @ Sun, 21 Jun 2020 20:43:48: 28000000 INFO @ Sun, 21 Jun 2020 20:43:49: 35000000 INFO @ Sun, 21 Jun 2020 20:43:51: 22000000 INFO @ Sun, 21 Jun 2020 20:43:53: 29000000 INFO @ Sun, 21 Jun 2020 20:43:54: 36000000 INFO @ Sun, 21 Jun 2020 20:43:56: 23000000 INFO @ Sun, 21 Jun 2020 20:43:58: 30000000 INFO @ Sun, 21 Jun 2020 20:43:59: 37000000 INFO @ Sun, 21 Jun 2020 20:44:01: 24000000 INFO @ Sun, 21 Jun 2020 20:44:02: 31000000 INFO @ Sun, 21 Jun 2020 20:44:03: 38000000 INFO @ Sun, 21 Jun 2020 20:44:06: 25000000 INFO @ Sun, 21 Jun 2020 20:44:07: 32000000 INFO @ Sun, 21 Jun 2020 20:44:08: 39000000 INFO @ Sun, 21 Jun 2020 20:44:11: 26000000 INFO @ Sun, 21 Jun 2020 20:44:12: 33000000 INFO @ Sun, 21 Jun 2020 20:44:13: 40000000 INFO @ Sun, 21 Jun 2020 20:44:15: 27000000 INFO @ Sun, 21 Jun 2020 20:44:16: 34000000 INFO @ Sun, 21 Jun 2020 20:44:18: 41000000 INFO @ Sun, 21 Jun 2020 20:44:20: 28000000 INFO @ Sun, 21 Jun 2020 20:44:21: 35000000 INFO @ Sun, 21 Jun 2020 20:44:23: 42000000 INFO @ Sun, 21 Jun 2020 20:44:25: 29000000 INFO @ Sun, 21 Jun 2020 20:44:26: 36000000 INFO @ Sun, 21 Jun 2020 20:44:27: 43000000 INFO @ Sun, 21 Jun 2020 20:44:30: 30000000 INFO @ Sun, 21 Jun 2020 20:44:31: 37000000 INFO @ Sun, 21 Jun 2020 20:44:32: 44000000 INFO @ Sun, 21 Jun 2020 20:44:35: 31000000 INFO @ Sun, 21 Jun 2020 20:44:35: 38000000 INFO @ Sun, 21 Jun 2020 20:44:37: 45000000 INFO @ Sun, 21 Jun 2020 20:44:40: 32000000 INFO @ Sun, 21 Jun 2020 20:44:40: 39000000 INFO @ Sun, 21 Jun 2020 20:44:42: 46000000 INFO @ Sun, 21 Jun 2020 20:44:44: 33000000 INFO @ Sun, 21 Jun 2020 20:44:45: 40000000 INFO @ Sun, 21 Jun 2020 20:44:46: 47000000 INFO @ Sun, 21 Jun 2020 20:44:49: 34000000 INFO @ Sun, 21 Jun 2020 20:44:50: 41000000 INFO @ Sun, 21 Jun 2020 20:44:51: 48000000 INFO @ Sun, 21 Jun 2020 20:44:54: 35000000 INFO @ Sun, 21 Jun 2020 20:44:55: 42000000 INFO @ Sun, 21 Jun 2020 20:44:56: 49000000 INFO @ Sun, 21 Jun 2020 20:44:59: 36000000 INFO @ Sun, 21 Jun 2020 20:45:00: 43000000 INFO @ Sun, 21 Jun 2020 20:45:01: 50000000 INFO @ Sun, 21 Jun 2020 20:45:04: 37000000 INFO @ Sun, 21 Jun 2020 20:45:05: 44000000 INFO @ Sun, 21 Jun 2020 20:45:05: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:45:05: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:45:05: #1 total tags in treatment: 50830797 INFO @ Sun, 21 Jun 2020 20:45:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:45:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:45:06: #1 tags after filtering in treatment: 50830772 INFO @ Sun, 21 Jun 2020 20:45:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:45:06: #1 finished! INFO @ Sun, 21 Jun 2020 20:45:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:45:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:45:09: 38000000 INFO @ Sun, 21 Jun 2020 20:45:09: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:45:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:45:09: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 20:45:09: 45000000 cut: /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:45:13: 39000000 INFO @ Sun, 21 Jun 2020 20:45:14: 46000000 INFO @ Sun, 21 Jun 2020 20:45:18: 40000000 INFO @ Sun, 21 Jun 2020 20:45:19: 47000000 INFO @ Sun, 21 Jun 2020 20:45:23: 41000000 INFO @ Sun, 21 Jun 2020 20:45:24: 48000000 INFO @ Sun, 21 Jun 2020 20:45:28: 42000000 INFO @ Sun, 21 Jun 2020 20:45:28: 49000000 INFO @ Sun, 21 Jun 2020 20:45:33: 43000000 INFO @ Sun, 21 Jun 2020 20:45:33: 50000000 INFO @ Sun, 21 Jun 2020 20:45:38: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:45:38: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:45:38: #1 total tags in treatment: 50830797 INFO @ Sun, 21 Jun 2020 20:45:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:45:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:45:38: 44000000 INFO @ Sun, 21 Jun 2020 20:45:39: #1 tags after filtering in treatment: 50830772 INFO @ Sun, 21 Jun 2020 20:45:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:45:39: #1 finished! INFO @ Sun, 21 Jun 2020 20:45:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:45:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:45:42: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:45:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:45:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:45:43: 45000000 INFO @ Sun, 21 Jun 2020 20:45:47: 46000000 INFO @ Sun, 21 Jun 2020 20:45:52: 47000000 INFO @ Sun, 21 Jun 2020 20:45:57: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:46:02: 49000000 INFO @ Sun, 21 Jun 2020 20:46:07: 50000000 INFO @ Sun, 21 Jun 2020 20:46:11: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:46:11: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:46:11: #1 total tags in treatment: 50830797 INFO @ Sun, 21 Jun 2020 20:46:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:46:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:46:12: #1 tags after filtering in treatment: 50830772 INFO @ Sun, 21 Jun 2020 20:46:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:46:12: #1 finished! INFO @ Sun, 21 Jun 2020 20:46:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:46:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:46:15: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:46:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:46:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630321/SRX3630321.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。