Job ID = 6456486 SRX = SRX348471 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:53:46 prefetch.2.10.7: 1) Downloading 'SRR976155'... 2020-06-21T10:53:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:55:39 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:55:40 prefetch.2.10.7: 'SRR976155' is valid 2020-06-21T10:55:40 prefetch.2.10.7: 1) 'SRR976155' was downloaded successfully Read 14046061 spots for SRR976155/SRR976155.sra Written 14046061 spots for SRR976155/SRR976155.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:09 14046061 reads; of these: 14046061 (100.00%) were unpaired; of these: 13620890 (96.97%) aligned 0 times 336501 (2.40%) aligned exactly 1 time 88670 (0.63%) aligned >1 times 3.03% overall alignment rate Time searching: 00:01:09 Overall time: 00:01:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 85996 / 425171 = 0.2023 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:58:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:58:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:58:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:58:11: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 19:58:11: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 19:58:11: #1 total tags in treatment: 339175 INFO @ Sun, 21 Jun 2020 19:58:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:58:11: #1 tags after filtering in treatment: 338686 INFO @ Sun, 21 Jun 2020 19:58:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:58:11: #1 finished! INFO @ Sun, 21 Jun 2020 19:58:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:58:12: #2 number of paired peaks: 4854 INFO @ Sun, 21 Jun 2020 19:58:12: start model_add_line... INFO @ Sun, 21 Jun 2020 19:58:12: start X-correlation... INFO @ Sun, 21 Jun 2020 19:58:12: end of X-cor INFO @ Sun, 21 Jun 2020 19:58:12: #2 finished! INFO @ Sun, 21 Jun 2020 19:58:12: #2 predicted fragment length is 36 bps INFO @ Sun, 21 Jun 2020 19:58:12: #2 alternative fragment length(s) may be 36,571 bps INFO @ Sun, 21 Jun 2020 19:58:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.05_model.r WARNING @ Sun, 21 Jun 2020 19:58:12: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 19:58:12: #2 You may need to consider one of the other alternative d(s): 36,571 WARNING @ Sun, 21 Jun 2020 19:58:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 19:58:12: #3 Call peaks... INFO @ Sun, 21 Jun 2020 19:58:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 19:58:13: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 19:58:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.05_peaks.xls INFO @ Sun, 21 Jun 2020 19:58:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.05_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 19:58:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.05_summits.bed INFO @ Sun, 21 Jun 2020 19:58:13: Done! pass1 - making usageList (79 chroms): 1 millis pass2 - checking and writing primary data (1632 records, 4 fields): 6 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:58:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:58:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:58:41: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 19:58:41: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 19:58:41: #1 total tags in treatment: 339175 INFO @ Sun, 21 Jun 2020 19:58:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:58:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:58:41: #1 tags after filtering in treatment: 338686 INFO @ Sun, 21 Jun 2020 19:58:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:58:41: #1 finished! INFO @ Sun, 21 Jun 2020 19:58:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:58:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:58:41: #2 number of paired peaks: 4854 INFO @ Sun, 21 Jun 2020 19:58:41: start model_add_line... INFO @ Sun, 21 Jun 2020 19:58:41: start X-correlation... INFO @ Sun, 21 Jun 2020 19:58:41: end of X-cor INFO @ Sun, 21 Jun 2020 19:58:41: #2 finished! INFO @ Sun, 21 Jun 2020 19:58:41: #2 predicted fragment length is 36 bps INFO @ Sun, 21 Jun 2020 19:58:41: #2 alternative fragment length(s) may be 36,571 bps INFO @ Sun, 21 Jun 2020 19:58:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.10_model.r WARNING @ Sun, 21 Jun 2020 19:58:41: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 19:58:41: #2 You may need to consider one of the other alternative d(s): 36,571 WARNING @ Sun, 21 Jun 2020 19:58:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 19:58:41: #3 Call peaks... INFO @ Sun, 21 Jun 2020 19:58:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 19:58:42: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 19:58:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.10_peaks.xls INFO @ Sun, 21 Jun 2020 19:58:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.10_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 19:58:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.10_summits.bed INFO @ Sun, 21 Jun 2020 19:58:43: Done! pass1 - making usageList (66 chroms): 1 millis pass2 - checking and writing primary data (546 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:59:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:59:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:59:09: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:59:11: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 19:59:11: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 19:59:11: #1 total tags in treatment: 339175 INFO @ Sun, 21 Jun 2020 19:59:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:59:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:59:11: #1 tags after filtering in treatment: 338686 INFO @ Sun, 21 Jun 2020 19:59:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:59:11: #1 finished! INFO @ Sun, 21 Jun 2020 19:59:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:59:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:59:12: #2 number of paired peaks: 4854 INFO @ Sun, 21 Jun 2020 19:59:12: start model_add_line... INFO @ Sun, 21 Jun 2020 19:59:12: start X-correlation... INFO @ Sun, 21 Jun 2020 19:59:12: end of X-cor INFO @ Sun, 21 Jun 2020 19:59:12: #2 finished! INFO @ Sun, 21 Jun 2020 19:59:12: #2 predicted fragment length is 36 bps INFO @ Sun, 21 Jun 2020 19:59:12: #2 alternative fragment length(s) may be 36,571 bps INFO @ Sun, 21 Jun 2020 19:59:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.20_model.r WARNING @ Sun, 21 Jun 2020 19:59:12: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 19:59:12: #2 You may need to consider one of the other alternative d(s): 36,571 WARNING @ Sun, 21 Jun 2020 19:59:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 19:59:12: #3 Call peaks... INFO @ Sun, 21 Jun 2020 19:59:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 19:59:12: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 19:59:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.20_peaks.xls INFO @ Sun, 21 Jun 2020 19:59:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.20_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 19:59:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX348471/SRX348471.20_summits.bed INFO @ Sun, 21 Jun 2020 19:59:13: Done! pass1 - making usageList (53 chroms): 1 millis pass2 - checking and writing primary data (161 records, 4 fields): 3 millis CompletedMACS2peakCalling