Job ID = 12265343 SRX = SRX3467367 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:32 7512864 reads; of these: 7512864 (100.00%) were unpaired; of these: 4382109 (58.33%) aligned 0 times 2950526 (39.27%) aligned exactly 1 time 180229 (2.40%) aligned >1 times 41.67% overall alignment rate Time searching: 00:02:32 Overall time: 00:02:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 855108 / 3130755 = 0.2731 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:42:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:42:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:42:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:42:12: 1000000 INFO @ Sat, 03 Apr 2021 06:42:18: 2000000 INFO @ Sat, 03 Apr 2021 06:42:20: #1 tag size is determined as 97 bps INFO @ Sat, 03 Apr 2021 06:42:20: #1 tag size = 97 INFO @ Sat, 03 Apr 2021 06:42:20: #1 total tags in treatment: 2275647 INFO @ Sat, 03 Apr 2021 06:42:20: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:42:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:42:20: #1 tags after filtering in treatment: 2274965 INFO @ Sat, 03 Apr 2021 06:42:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:42:20: #1 finished! INFO @ Sat, 03 Apr 2021 06:42:20: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:42:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:42:20: #2 number of paired peaks: 8210 INFO @ Sat, 03 Apr 2021 06:42:20: start model_add_line... INFO @ Sat, 03 Apr 2021 06:42:20: start X-correlation... INFO @ Sat, 03 Apr 2021 06:42:20: end of X-cor INFO @ Sat, 03 Apr 2021 06:42:20: #2 finished! INFO @ Sat, 03 Apr 2021 06:42:20: #2 predicted fragment length is 120 bps INFO @ Sat, 03 Apr 2021 06:42:20: #2 alternative fragment length(s) may be 120 bps INFO @ Sat, 03 Apr 2021 06:42:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.05_model.r WARNING @ Sat, 03 Apr 2021 06:42:20: #2 Since the d (120) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:42:20: #2 You may need to consider one of the other alternative d(s): 120 WARNING @ Sat, 03 Apr 2021 06:42:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:42:20: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:42:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:42:26: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:42:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:42:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:42:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.05_summits.bed INFO @ Sat, 03 Apr 2021 06:42:29: Done! pass1 - making usageList (49 chroms): 2 millis pass2 - checking and writing primary data (11950 records, 4 fields): 14 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:42:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:42:35: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:42:35: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:42:42: 1000000 INFO @ Sat, 03 Apr 2021 06:42:49: 2000000 INFO @ Sat, 03 Apr 2021 06:42:51: #1 tag size is determined as 97 bps INFO @ Sat, 03 Apr 2021 06:42:51: #1 tag size = 97 INFO @ Sat, 03 Apr 2021 06:42:51: #1 total tags in treatment: 2275647 INFO @ Sat, 03 Apr 2021 06:42:51: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:42:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:42:51: #1 tags after filtering in treatment: 2274965 INFO @ Sat, 03 Apr 2021 06:42:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:42:51: #1 finished! INFO @ Sat, 03 Apr 2021 06:42:51: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:42:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:42:52: #2 number of paired peaks: 8210 INFO @ Sat, 03 Apr 2021 06:42:52: start model_add_line... INFO @ Sat, 03 Apr 2021 06:42:52: start X-correlation... INFO @ Sat, 03 Apr 2021 06:42:52: end of X-cor INFO @ Sat, 03 Apr 2021 06:42:52: #2 finished! INFO @ Sat, 03 Apr 2021 06:42:52: #2 predicted fragment length is 120 bps INFO @ Sat, 03 Apr 2021 06:42:52: #2 alternative fragment length(s) may be 120 bps INFO @ Sat, 03 Apr 2021 06:42:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.10_model.r WARNING @ Sat, 03 Apr 2021 06:42:52: #2 Since the d (120) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:42:52: #2 You may need to consider one of the other alternative d(s): 120 WARNING @ Sat, 03 Apr 2021 06:42:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:42:52: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:42:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:42:58: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:43:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:43:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:43:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.10_summits.bed INFO @ Sat, 03 Apr 2021 06:43:01: Done! pass1 - making usageList (27 chroms): 2 millis pass2 - checking and writing primary data (7081 records, 4 fields): 28 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:43:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:43:05: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:43:05: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:43:13: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:43:20: 2000000 INFO @ Sat, 03 Apr 2021 06:43:22: #1 tag size is determined as 97 bps INFO @ Sat, 03 Apr 2021 06:43:22: #1 tag size = 97 INFO @ Sat, 03 Apr 2021 06:43:22: #1 total tags in treatment: 2275647 INFO @ Sat, 03 Apr 2021 06:43:22: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:43:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:43:22: #1 tags after filtering in treatment: 2274965 INFO @ Sat, 03 Apr 2021 06:43:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:43:22: #1 finished! INFO @ Sat, 03 Apr 2021 06:43:22: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:43:22: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:43:22: #2 number of paired peaks: 8210 INFO @ Sat, 03 Apr 2021 06:43:22: start model_add_line... INFO @ Sat, 03 Apr 2021 06:43:22: start X-correlation... INFO @ Sat, 03 Apr 2021 06:43:22: end of X-cor INFO @ Sat, 03 Apr 2021 06:43:22: #2 finished! INFO @ Sat, 03 Apr 2021 06:43:22: #2 predicted fragment length is 120 bps INFO @ Sat, 03 Apr 2021 06:43:22: #2 alternative fragment length(s) may be 120 bps INFO @ Sat, 03 Apr 2021 06:43:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.20_model.r WARNING @ Sat, 03 Apr 2021 06:43:22: #2 Since the d (120) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:43:22: #2 You may need to consider one of the other alternative d(s): 120 WARNING @ Sat, 03 Apr 2021 06:43:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:43:22: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:43:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:43:28: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:43:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:43:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:43:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3467367/SRX3467367.20_summits.bed INFO @ Sat, 03 Apr 2021 06:43:31: Done! pass1 - making usageList (19 chroms): 1 millis pass2 - checking and writing primary data (3061 records, 4 fields): 15 millis CompletedMACS2peakCalling