Job ID = 6456335 SRX = SRX336284 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:40:08 prefetch.2.10.7: 1) Downloading 'SRR953624'... 2020-06-21T10:40:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:45:24 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:45:24 prefetch.2.10.7: 1) 'SRR953624' was downloaded successfully Read 22529054 spots for SRR953624/SRR953624.sra Written 22529054 spots for SRR953624/SRR953624.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:04 22529054 reads; of these: 22529054 (100.00%) were unpaired; of these: 4442525 (19.72%) aligned 0 times 15661178 (69.52%) aligned exactly 1 time 2425351 (10.77%) aligned >1 times 80.28% overall alignment rate Time searching: 00:04:04 Overall time: 00:04:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4212429 / 18086529 = 0.2329 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:54:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:54:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:54:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:54:56: 1000000 INFO @ Sun, 21 Jun 2020 19:55:03: 2000000 INFO @ Sun, 21 Jun 2020 19:55:10: 3000000 INFO @ Sun, 21 Jun 2020 19:55:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:55:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:55:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:55:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:55:23: 5000000 INFO @ Sun, 21 Jun 2020 19:55:27: 1000000 INFO @ Sun, 21 Jun 2020 19:55:30: 6000000 INFO @ Sun, 21 Jun 2020 19:55:33: 2000000 INFO @ Sun, 21 Jun 2020 19:55:37: 7000000 INFO @ Sun, 21 Jun 2020 19:55:40: 3000000 INFO @ Sun, 21 Jun 2020 19:55:45: 8000000 INFO @ Sun, 21 Jun 2020 19:55:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:55:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:55:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:55:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:55:52: 9000000 INFO @ Sun, 21 Jun 2020 19:55:53: 5000000 INFO @ Sun, 21 Jun 2020 19:55:57: 1000000 INFO @ Sun, 21 Jun 2020 19:55:59: 10000000 INFO @ Sun, 21 Jun 2020 19:56:00: 6000000 INFO @ Sun, 21 Jun 2020 19:56:03: 2000000 INFO @ Sun, 21 Jun 2020 19:56:07: 11000000 INFO @ Sun, 21 Jun 2020 19:56:07: 7000000 INFO @ Sun, 21 Jun 2020 19:56:10: 3000000 INFO @ Sun, 21 Jun 2020 19:56:14: 8000000 INFO @ Sun, 21 Jun 2020 19:56:15: 12000000 INFO @ Sun, 21 Jun 2020 19:56:17: 4000000 INFO @ Sun, 21 Jun 2020 19:56:21: 9000000 INFO @ Sun, 21 Jun 2020 19:56:22: 13000000 INFO @ Sun, 21 Jun 2020 19:56:23: 5000000 INFO @ Sun, 21 Jun 2020 19:56:27: 10000000 INFO @ Sun, 21 Jun 2020 19:56:28: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 19:56:28: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 19:56:28: #1 total tags in treatment: 13874100 INFO @ Sun, 21 Jun 2020 19:56:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:56:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:56:29: #1 tags after filtering in treatment: 13874098 INFO @ Sun, 21 Jun 2020 19:56:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:56:29: #1 finished! INFO @ Sun, 21 Jun 2020 19:56:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:56:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:56:30: 6000000 INFO @ Sun, 21 Jun 2020 19:56:30: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 19:56:30: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:56:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:56:34: 11000000 INFO @ Sun, 21 Jun 2020 19:56:36: 7000000 INFO @ Sun, 21 Jun 2020 19:56:41: 12000000 INFO @ Sun, 21 Jun 2020 19:56:43: 8000000 INFO @ Sun, 21 Jun 2020 19:56:47: 13000000 INFO @ Sun, 21 Jun 2020 19:56:49: 9000000 INFO @ Sun, 21 Jun 2020 19:56:53: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 19:56:53: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 19:56:53: #1 total tags in treatment: 13874100 INFO @ Sun, 21 Jun 2020 19:56:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:56:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:56:53: #1 tags after filtering in treatment: 13874098 INFO @ Sun, 21 Jun 2020 19:56:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:56:53: #1 finished! INFO @ Sun, 21 Jun 2020 19:56:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:56:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:56:54: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 19:56:54: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:56:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:56:55: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:57:01: 11000000 INFO @ Sun, 21 Jun 2020 19:57:08: 12000000 INFO @ Sun, 21 Jun 2020 19:57:13: 13000000 INFO @ Sun, 21 Jun 2020 19:57:19: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 19:57:19: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 19:57:19: #1 total tags in treatment: 13874100 INFO @ Sun, 21 Jun 2020 19:57:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:57:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:57:19: #1 tags after filtering in treatment: 13874098 INFO @ Sun, 21 Jun 2020 19:57:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:57:19: #1 finished! INFO @ Sun, 21 Jun 2020 19:57:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:57:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:57:20: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 19:57:20: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:57:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336284/SRX336284.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。