Job ID = 6456331 SRX = SRX336283 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:49:46 prefetch.2.10.7: 1) Downloading 'SRR953623'... 2020-06-21T10:49:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:54:05 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:54:05 prefetch.2.10.7: 1) 'SRR953623' was downloaded successfully Read 22251934 spots for SRR953623/SRR953623.sra Written 22251934 spots for SRR953623/SRR953623.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:53 22251934 reads; of these: 22251934 (100.00%) were unpaired; of these: 973302 (4.37%) aligned 0 times 15054195 (67.65%) aligned exactly 1 time 6224437 (27.97%) aligned >1 times 95.63% overall alignment rate Time searching: 00:05:53 Overall time: 00:05:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5988539 / 21278632 = 0.2814 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:06:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:06:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:06:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:06:15: 1000000 INFO @ Sun, 21 Jun 2020 20:06:20: 2000000 INFO @ Sun, 21 Jun 2020 20:06:26: 3000000 INFO @ Sun, 21 Jun 2020 20:06:31: 4000000 INFO @ Sun, 21 Jun 2020 20:06:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:06:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:06:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:06:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:06:42: 6000000 INFO @ Sun, 21 Jun 2020 20:06:45: 1000000 INFO @ Sun, 21 Jun 2020 20:06:48: 7000000 INFO @ Sun, 21 Jun 2020 20:06:51: 2000000 INFO @ Sun, 21 Jun 2020 20:06:54: 8000000 INFO @ Sun, 21 Jun 2020 20:06:57: 3000000 INFO @ Sun, 21 Jun 2020 20:07:00: 9000000 INFO @ Sun, 21 Jun 2020 20:07:03: 4000000 INFO @ Sun, 21 Jun 2020 20:07:06: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:07:09: 5000000 INFO @ Sun, 21 Jun 2020 20:07:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:07:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:07:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:07:12: 11000000 INFO @ Sun, 21 Jun 2020 20:07:15: 6000000 INFO @ Sun, 21 Jun 2020 20:07:16: 1000000 INFO @ Sun, 21 Jun 2020 20:07:18: 12000000 INFO @ Sun, 21 Jun 2020 20:07:21: 7000000 INFO @ Sun, 21 Jun 2020 20:07:23: 2000000 INFO @ Sun, 21 Jun 2020 20:07:24: 13000000 INFO @ Sun, 21 Jun 2020 20:07:27: 8000000 INFO @ Sun, 21 Jun 2020 20:07:29: 3000000 INFO @ Sun, 21 Jun 2020 20:07:30: 14000000 INFO @ Sun, 21 Jun 2020 20:07:33: 9000000 INFO @ Sun, 21 Jun 2020 20:07:36: 15000000 INFO @ Sun, 21 Jun 2020 20:07:36: 4000000 INFO @ Sun, 21 Jun 2020 20:07:38: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 20:07:38: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 20:07:38: #1 total tags in treatment: 15290093 INFO @ Sun, 21 Jun 2020 20:07:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:38: #1 tags after filtering in treatment: 15290093 INFO @ Sun, 21 Jun 2020 20:07:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:38: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:07:39: 10000000 INFO @ Sun, 21 Jun 2020 20:07:39: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 20:07:39: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:07:43: 5000000 INFO @ Sun, 21 Jun 2020 20:07:45: 11000000 INFO @ Sun, 21 Jun 2020 20:07:50: 6000000 INFO @ Sun, 21 Jun 2020 20:07:51: 12000000 INFO @ Sun, 21 Jun 2020 20:07:56: 7000000 INFO @ Sun, 21 Jun 2020 20:07:57: 13000000 INFO @ Sun, 21 Jun 2020 20:08:03: 14000000 INFO @ Sun, 21 Jun 2020 20:08:03: 8000000 INFO @ Sun, 21 Jun 2020 20:08:09: 15000000 INFO @ Sun, 21 Jun 2020 20:08:10: 9000000 INFO @ Sun, 21 Jun 2020 20:08:10: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 20:08:10: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 20:08:10: #1 total tags in treatment: 15290093 INFO @ Sun, 21 Jun 2020 20:08:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:11: #1 tags after filtering in treatment: 15290093 INFO @ Sun, 21 Jun 2020 20:08:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:11: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:12: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 20:08:12: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:08:16: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:08:22: 11000000 INFO @ Sun, 21 Jun 2020 20:08:29: 12000000 INFO @ Sun, 21 Jun 2020 20:08:36: 13000000 INFO @ Sun, 21 Jun 2020 20:08:42: 14000000 INFO @ Sun, 21 Jun 2020 20:08:48: 15000000 INFO @ Sun, 21 Jun 2020 20:08:50: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 20:08:50: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 20:08:50: #1 total tags in treatment: 15290093 INFO @ Sun, 21 Jun 2020 20:08:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:51: #1 tags after filtering in treatment: 15290093 INFO @ Sun, 21 Jun 2020 20:08:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:51: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:52: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 20:08:52: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336283/SRX336283.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。