Job ID = 6456329 SRX = SRX336281 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:53:16 prefetch.2.10.7: 1) Downloading 'SRR953621'... 2020-06-21T10:53:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:57:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:57:04 prefetch.2.10.7: 1) 'SRR953621' was downloaded successfully Read 21914988 spots for SRR953621/SRR953621.sra Written 21914988 spots for SRR953621/SRR953621.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:56 21914988 reads; of these: 21914988 (100.00%) were unpaired; of these: 2170291 (9.90%) aligned 0 times 17713589 (80.83%) aligned exactly 1 time 2031108 (9.27%) aligned >1 times 90.10% overall alignment rate Time searching: 00:03:56 Overall time: 00:03:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3723873 / 19744697 = 0.1886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:06:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:06:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:06:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:06:37: 1000000 INFO @ Sun, 21 Jun 2020 20:06:42: 2000000 INFO @ Sun, 21 Jun 2020 20:06:46: 3000000 INFO @ Sun, 21 Jun 2020 20:06:51: 4000000 INFO @ Sun, 21 Jun 2020 20:06:55: 5000000 INFO @ Sun, 21 Jun 2020 20:07:00: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:07:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:07:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:07:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:07:05: 7000000 INFO @ Sun, 21 Jun 2020 20:07:07: 1000000 INFO @ Sun, 21 Jun 2020 20:07:09: 8000000 INFO @ Sun, 21 Jun 2020 20:07:12: 2000000 INFO @ Sun, 21 Jun 2020 20:07:14: 9000000 INFO @ Sun, 21 Jun 2020 20:07:17: 3000000 INFO @ Sun, 21 Jun 2020 20:07:19: 10000000 INFO @ Sun, 21 Jun 2020 20:07:22: 4000000 INFO @ Sun, 21 Jun 2020 20:07:24: 11000000 INFO @ Sun, 21 Jun 2020 20:07:26: 5000000 INFO @ Sun, 21 Jun 2020 20:07:28: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:07:31: 6000000 INFO @ Sun, 21 Jun 2020 20:07:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:07:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:07:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:07:33: 13000000 INFO @ Sun, 21 Jun 2020 20:07:36: 7000000 INFO @ Sun, 21 Jun 2020 20:07:37: 1000000 INFO @ Sun, 21 Jun 2020 20:07:38: 14000000 INFO @ Sun, 21 Jun 2020 20:07:41: 8000000 INFO @ Sun, 21 Jun 2020 20:07:42: 2000000 INFO @ Sun, 21 Jun 2020 20:07:43: 15000000 INFO @ Sun, 21 Jun 2020 20:07:45: 9000000 INFO @ Sun, 21 Jun 2020 20:07:47: 3000000 INFO @ Sun, 21 Jun 2020 20:07:48: 16000000 INFO @ Sun, 21 Jun 2020 20:07:48: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 20:07:48: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 20:07:48: #1 total tags in treatment: 16020824 INFO @ Sun, 21 Jun 2020 20:07:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:48: #1 tags after filtering in treatment: 16020731 INFO @ Sun, 21 Jun 2020 20:07:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:48: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:07:49: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 20:07:49: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:07:50: 10000000 INFO @ Sun, 21 Jun 2020 20:07:52: 4000000 INFO @ Sun, 21 Jun 2020 20:07:55: 11000000 INFO @ Sun, 21 Jun 2020 20:07:56: 5000000 INFO @ Sun, 21 Jun 2020 20:08:00: 12000000 INFO @ Sun, 21 Jun 2020 20:08:01: 6000000 INFO @ Sun, 21 Jun 2020 20:08:05: 13000000 INFO @ Sun, 21 Jun 2020 20:08:06: 7000000 INFO @ Sun, 21 Jun 2020 20:08:09: 14000000 INFO @ Sun, 21 Jun 2020 20:08:10: 8000000 INFO @ Sun, 21 Jun 2020 20:08:14: 15000000 INFO @ Sun, 21 Jun 2020 20:08:15: 9000000 INFO @ Sun, 21 Jun 2020 20:08:19: 16000000 INFO @ Sun, 21 Jun 2020 20:08:19: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 20:08:19: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 20:08:19: #1 total tags in treatment: 16020824 INFO @ Sun, 21 Jun 2020 20:08:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:20: #1 tags after filtering in treatment: 16020731 INFO @ Sun, 21 Jun 2020 20:08:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:20: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:20: 10000000 INFO @ Sun, 21 Jun 2020 20:08:21: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 20:08:21: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:08:25: 11000000 INFO @ Sun, 21 Jun 2020 20:08:29: 12000000 INFO @ Sun, 21 Jun 2020 20:08:34: 13000000 INFO @ Sun, 21 Jun 2020 20:08:39: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:08:43: 15000000 INFO @ Sun, 21 Jun 2020 20:08:48: 16000000 INFO @ Sun, 21 Jun 2020 20:08:48: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 20:08:48: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 20:08:48: #1 total tags in treatment: 16020824 INFO @ Sun, 21 Jun 2020 20:08:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:49: #1 tags after filtering in treatment: 16020731 INFO @ Sun, 21 Jun 2020 20:08:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:49: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:50: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 20:08:50: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX336281/SRX336281.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。