Job ID = 6456321 SRX = SRX335519 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:35:14 prefetch.2.10.7: 1) Downloading 'SRR952861'... 2020-06-21T10:35:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:41:41 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:41:41 prefetch.2.10.7: 1) 'SRR952861' was downloaded successfully Read 24355341 spots for SRR952861/SRR952861.sra Written 24355341 spots for SRR952861/SRR952861.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 24355341 reads; of these: 24355341 (100.00%) were unpaired; of these: 1544219 (6.34%) aligned 0 times 20105316 (82.55%) aligned exactly 1 time 2705806 (11.11%) aligned >1 times 93.66% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5059119 / 22811122 = 0.2218 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:53:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:53:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:53:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:53:16: 1000000 INFO @ Sun, 21 Jun 2020 19:53:21: 2000000 INFO @ Sun, 21 Jun 2020 19:53:26: 3000000 INFO @ Sun, 21 Jun 2020 19:53:30: 4000000 INFO @ Sun, 21 Jun 2020 19:53:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:53:40: 6000000 INFO @ Sun, 21 Jun 2020 19:53:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:53:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:53:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:53:46: 7000000 INFO @ Sun, 21 Jun 2020 19:53:46: 1000000 INFO @ Sun, 21 Jun 2020 19:53:51: 8000000 INFO @ Sun, 21 Jun 2020 19:53:51: 2000000 INFO @ Sun, 21 Jun 2020 19:53:56: 9000000 INFO @ Sun, 21 Jun 2020 19:53:56: 3000000 INFO @ Sun, 21 Jun 2020 19:54:01: 10000000 INFO @ Sun, 21 Jun 2020 19:54:01: 4000000 INFO @ Sun, 21 Jun 2020 19:54:06: 11000000 INFO @ Sun, 21 Jun 2020 19:54:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:54:11: 6000000 INFO @ Sun, 21 Jun 2020 19:54:11: 12000000 INFO @ Sun, 21 Jun 2020 19:54:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:54:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:54:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:54:16: 7000000 INFO @ Sun, 21 Jun 2020 19:54:16: 13000000 INFO @ Sun, 21 Jun 2020 19:54:16: 1000000 INFO @ Sun, 21 Jun 2020 19:54:21: 8000000 INFO @ Sun, 21 Jun 2020 19:54:21: 14000000 INFO @ Sun, 21 Jun 2020 19:54:21: 2000000 INFO @ Sun, 21 Jun 2020 19:54:26: 9000000 INFO @ Sun, 21 Jun 2020 19:54:26: 3000000 INFO @ Sun, 21 Jun 2020 19:54:27: 15000000 INFO @ Sun, 21 Jun 2020 19:54:31: 10000000 INFO @ Sun, 21 Jun 2020 19:54:32: 4000000 INFO @ Sun, 21 Jun 2020 19:54:32: 16000000 INFO @ Sun, 21 Jun 2020 19:54:36: 11000000 INFO @ Sun, 21 Jun 2020 19:54:37: 17000000 INFO @ Sun, 21 Jun 2020 19:54:37: 5000000 INFO @ Sun, 21 Jun 2020 19:54:41: 12000000 INFO @ Sun, 21 Jun 2020 19:54:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:54:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:54:41: #1 total tags in treatment: 17752003 INFO @ Sun, 21 Jun 2020 19:54:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:54:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:54:42: #1 tags after filtering in treatment: 17751992 INFO @ Sun, 21 Jun 2020 19:54:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:54:42: #1 finished! INFO @ Sun, 21 Jun 2020 19:54:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:54:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:54:42: 6000000 INFO @ Sun, 21 Jun 2020 19:54:43: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:54:43: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:54:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:54:46: 13000000 INFO @ Sun, 21 Jun 2020 19:54:47: 7000000 INFO @ Sun, 21 Jun 2020 19:54:51: 14000000 INFO @ Sun, 21 Jun 2020 19:54:52: 8000000 INFO @ Sun, 21 Jun 2020 19:54:56: 15000000 INFO @ Sun, 21 Jun 2020 19:54:57: 9000000 INFO @ Sun, 21 Jun 2020 19:55:01: 16000000 INFO @ Sun, 21 Jun 2020 19:55:02: 10000000 INFO @ Sun, 21 Jun 2020 19:55:06: 17000000 INFO @ Sun, 21 Jun 2020 19:55:08: 11000000 INFO @ Sun, 21 Jun 2020 19:55:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:55:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:55:10: #1 total tags in treatment: 17752003 INFO @ Sun, 21 Jun 2020 19:55:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:55:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:55:11: #1 tags after filtering in treatment: 17751992 INFO @ Sun, 21 Jun 2020 19:55:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:55:11: #1 finished! INFO @ Sun, 21 Jun 2020 19:55:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:55:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:55:12: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:55:12: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:55:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:55:13: 12000000 INFO @ Sun, 21 Jun 2020 19:55:18: 13000000 INFO @ Sun, 21 Jun 2020 19:55:23: 14000000 INFO @ Sun, 21 Jun 2020 19:55:28: 15000000 INFO @ Sun, 21 Jun 2020 19:55:33: 16000000 INFO @ Sun, 21 Jun 2020 19:55:38: 17000000 INFO @ Sun, 21 Jun 2020 19:55:42: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:55:42: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:55:42: #1 total tags in treatment: 17752003 INFO @ Sun, 21 Jun 2020 19:55:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:55:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:55:43: #1 tags after filtering in treatment: 17751992 INFO @ Sun, 21 Jun 2020 19:55:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:55:43: #1 finished! INFO @ Sun, 21 Jun 2020 19:55:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:55:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:55:44: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:55:44: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:55:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335519/SRX335519.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。