Job ID = 6456315 SRX = SRX335514 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:00:46 prefetch.2.10.7: 1) Downloading 'SRR952856'... 2020-06-21T11:00:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:14:39 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:14:39 prefetch.2.10.7: 1) 'SRR952856' was downloaded successfully Read 84993195 spots for SRR952856/SRR952856.sra Written 84993195 spots for SRR952856/SRR952856.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:18 84993195 reads; of these: 84993195 (100.00%) were unpaired; of these: 3426643 (4.03%) aligned 0 times 63019679 (74.15%) aligned exactly 1 time 18546873 (21.82%) aligned >1 times 95.97% overall alignment rate Time searching: 00:25:19 Overall time: 00:25:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 36539431 / 81566552 = 0.4480 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:04:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:04:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:04:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:04:23: 1000000 INFO @ Sun, 21 Jun 2020 21:04:33: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:04:42: 3000000 INFO @ Sun, 21 Jun 2020 21:04:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:04:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:04:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:04:52: 4000000 INFO @ Sun, 21 Jun 2020 21:04:52: 1000000 INFO @ Sun, 21 Jun 2020 21:05:02: 5000000 INFO @ Sun, 21 Jun 2020 21:05:02: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:05:12: 6000000 INFO @ Sun, 21 Jun 2020 21:05:12: 3000000 INFO @ Sun, 21 Jun 2020 21:05:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:05:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:05:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:05:22: 7000000 INFO @ Sun, 21 Jun 2020 21:05:22: 4000000 INFO @ Sun, 21 Jun 2020 21:05:22: 1000000 INFO @ Sun, 21 Jun 2020 21:05:32: 2000000 INFO @ Sun, 21 Jun 2020 21:05:32: 8000000 INFO @ Sun, 21 Jun 2020 21:05:32: 5000000 INFO @ Sun, 21 Jun 2020 21:05:41: 3000000 INFO @ Sun, 21 Jun 2020 21:05:42: 9000000 INFO @ Sun, 21 Jun 2020 21:05:42: 6000000 INFO @ Sun, 21 Jun 2020 21:05:51: 4000000 INFO @ Sun, 21 Jun 2020 21:05:52: 10000000 INFO @ Sun, 21 Jun 2020 21:05:53: 7000000 INFO @ Sun, 21 Jun 2020 21:06:01: 5000000 INFO @ Sun, 21 Jun 2020 21:06:03: 11000000 INFO @ Sun, 21 Jun 2020 21:06:03: 8000000 INFO @ Sun, 21 Jun 2020 21:06:10: 6000000 INFO @ Sun, 21 Jun 2020 21:06:13: 12000000 INFO @ Sun, 21 Jun 2020 21:06:13: 9000000 INFO @ Sun, 21 Jun 2020 21:06:20: 7000000 INFO @ Sun, 21 Jun 2020 21:06:23: 13000000 INFO @ Sun, 21 Jun 2020 21:06:23: 10000000 INFO @ Sun, 21 Jun 2020 21:06:29: 8000000 INFO @ Sun, 21 Jun 2020 21:06:33: 14000000 INFO @ Sun, 21 Jun 2020 21:06:33: 11000000 INFO @ Sun, 21 Jun 2020 21:06:38: 9000000 INFO @ Sun, 21 Jun 2020 21:06:43: 15000000 INFO @ Sun, 21 Jun 2020 21:06:43: 12000000 INFO @ Sun, 21 Jun 2020 21:06:47: 10000000 INFO @ Sun, 21 Jun 2020 21:06:53: 16000000 INFO @ Sun, 21 Jun 2020 21:06:53: 13000000 INFO @ Sun, 21 Jun 2020 21:06:56: 11000000 INFO @ Sun, 21 Jun 2020 21:07:02: 17000000 INFO @ Sun, 21 Jun 2020 21:07:03: 14000000 INFO @ Sun, 21 Jun 2020 21:07:05: 12000000 INFO @ Sun, 21 Jun 2020 21:07:12: 18000000 INFO @ Sun, 21 Jun 2020 21:07:12: 15000000 INFO @ Sun, 21 Jun 2020 21:07:14: 13000000 INFO @ Sun, 21 Jun 2020 21:07:22: 19000000 INFO @ Sun, 21 Jun 2020 21:07:22: 16000000 INFO @ Sun, 21 Jun 2020 21:07:23: 14000000 INFO @ Sun, 21 Jun 2020 21:07:31: 15000000 INFO @ Sun, 21 Jun 2020 21:07:31: 20000000 INFO @ Sun, 21 Jun 2020 21:07:32: 17000000 INFO @ Sun, 21 Jun 2020 21:07:40: 16000000 INFO @ Sun, 21 Jun 2020 21:07:41: 21000000 INFO @ Sun, 21 Jun 2020 21:07:41: 18000000 INFO @ Sun, 21 Jun 2020 21:07:47: 17000000 INFO @ Sun, 21 Jun 2020 21:07:50: 22000000 INFO @ Sun, 21 Jun 2020 21:07:50: 19000000 INFO @ Sun, 21 Jun 2020 21:07:55: 18000000 INFO @ Sun, 21 Jun 2020 21:07:58: 23000000 INFO @ Sun, 21 Jun 2020 21:07:58: 20000000 INFO @ Sun, 21 Jun 2020 21:08:02: 19000000 INFO @ Sun, 21 Jun 2020 21:08:05: 24000000 INFO @ Sun, 21 Jun 2020 21:08:05: 21000000 INFO @ Sun, 21 Jun 2020 21:08:09: 20000000 INFO @ Sun, 21 Jun 2020 21:08:13: 25000000 INFO @ Sun, 21 Jun 2020 21:08:13: 22000000 INFO @ Sun, 21 Jun 2020 21:08:16: 21000000 INFO @ Sun, 21 Jun 2020 21:08:20: 26000000 INFO @ Sun, 21 Jun 2020 21:08:20: 23000000 INFO @ Sun, 21 Jun 2020 21:08:23: 22000000 INFO @ Sun, 21 Jun 2020 21:08:28: 27000000 INFO @ Sun, 21 Jun 2020 21:08:28: 24000000 INFO @ Sun, 21 Jun 2020 21:08:29: 23000000 INFO @ Sun, 21 Jun 2020 21:08:35: 28000000 INFO @ Sun, 21 Jun 2020 21:08:35: 25000000 INFO @ Sun, 21 Jun 2020 21:08:36: 24000000 INFO @ Sun, 21 Jun 2020 21:08:42: 26000000 INFO @ Sun, 21 Jun 2020 21:08:42: 29000000 INFO @ Sun, 21 Jun 2020 21:08:43: 25000000 INFO @ Sun, 21 Jun 2020 21:08:49: 30000000 INFO @ Sun, 21 Jun 2020 21:08:49: 26000000 INFO @ Sun, 21 Jun 2020 21:08:50: 27000000 INFO @ Sun, 21 Jun 2020 21:08:56: 27000000 INFO @ Sun, 21 Jun 2020 21:08:56: 31000000 INFO @ Sun, 21 Jun 2020 21:08:57: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:09:02: 28000000 INFO @ Sun, 21 Jun 2020 21:09:03: 32000000 INFO @ Sun, 21 Jun 2020 21:09:04: 29000000 INFO @ Sun, 21 Jun 2020 21:09:08: 29000000 INFO @ Sun, 21 Jun 2020 21:09:09: 33000000 INFO @ Sun, 21 Jun 2020 21:09:12: 30000000 INFO @ Sun, 21 Jun 2020 21:09:15: 30000000 INFO @ Sun, 21 Jun 2020 21:09:16: 34000000 INFO @ Sun, 21 Jun 2020 21:09:19: 31000000 INFO @ Sun, 21 Jun 2020 21:09:21: 31000000 INFO @ Sun, 21 Jun 2020 21:09:23: 35000000 INFO @ Sun, 21 Jun 2020 21:09:26: 32000000 INFO @ Sun, 21 Jun 2020 21:09:28: 32000000 INFO @ Sun, 21 Jun 2020 21:09:30: 36000000 INFO @ Sun, 21 Jun 2020 21:09:34: 33000000 INFO @ Sun, 21 Jun 2020 21:09:34: 33000000 INFO @ Sun, 21 Jun 2020 21:09:39: 37000000 INFO @ Sun, 21 Jun 2020 21:09:41: 34000000 INFO @ Sun, 21 Jun 2020 21:09:41: 34000000 INFO @ Sun, 21 Jun 2020 21:09:46: 38000000 INFO @ Sun, 21 Jun 2020 21:09:48: 35000000 INFO @ Sun, 21 Jun 2020 21:09:48: 35000000 INFO @ Sun, 21 Jun 2020 21:09:53: 39000000 INFO @ Sun, 21 Jun 2020 21:09:55: 36000000 INFO @ Sun, 21 Jun 2020 21:09:55: 36000000 INFO @ Sun, 21 Jun 2020 21:09:59: 40000000 INFO @ Sun, 21 Jun 2020 21:10:03: 37000000 INFO @ Sun, 21 Jun 2020 21:10:03: 37000000 INFO @ Sun, 21 Jun 2020 21:10:07: 41000000 INFO @ Sun, 21 Jun 2020 21:10:10: 38000000 INFO @ Sun, 21 Jun 2020 21:10:10: 38000000 INFO @ Sun, 21 Jun 2020 21:10:14: 42000000 INFO @ Sun, 21 Jun 2020 21:10:17: 39000000 INFO @ Sun, 21 Jun 2020 21:10:18: 39000000 INFO @ Sun, 21 Jun 2020 21:10:21: 43000000 INFO @ Sun, 21 Jun 2020 21:10:24: 40000000 INFO @ Sun, 21 Jun 2020 21:10:24: 40000000 INFO @ Sun, 21 Jun 2020 21:10:28: 44000000 INFO @ Sun, 21 Jun 2020 21:10:31: 41000000 INFO @ Sun, 21 Jun 2020 21:10:31: 41000000 INFO @ Sun, 21 Jun 2020 21:10:36: 45000000 INFO @ Sun, 21 Jun 2020 21:10:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:10:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:10:36: #1 total tags in treatment: 45027121 INFO @ Sun, 21 Jun 2020 21:10:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:10:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:10:38: #1 tags after filtering in treatment: 45027120 INFO @ Sun, 21 Jun 2020 21:10:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:10:38: #1 finished! INFO @ Sun, 21 Jun 2020 21:10:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:10:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:10:38: 42000000 INFO @ Sun, 21 Jun 2020 21:10:38: 42000000 INFO @ Sun, 21 Jun 2020 21:10:41: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 21:10:41: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:10:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:10:45: 43000000 INFO @ Sun, 21 Jun 2020 21:10:45: 43000000 INFO @ Sun, 21 Jun 2020 21:10:51: 44000000 INFO @ Sun, 21 Jun 2020 21:10:52: 44000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:10:58: 45000000 INFO @ Sun, 21 Jun 2020 21:10:59: 45000000 INFO @ Sun, 21 Jun 2020 21:10:59: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:10:59: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:10:59: #1 total tags in treatment: 45027121 INFO @ Sun, 21 Jun 2020 21:10:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:10:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:10:59: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:10:59: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:10:59: #1 total tags in treatment: 45027121 INFO @ Sun, 21 Jun 2020 21:10:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:10:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:11:00: #1 tags after filtering in treatment: 45027120 INFO @ Sun, 21 Jun 2020 21:11:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:11:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:11:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:11:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:11:01: #1 tags after filtering in treatment: 45027120 INFO @ Sun, 21 Jun 2020 21:11:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:11:01: #1 finished! INFO @ Sun, 21 Jun 2020 21:11:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:11:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:11:03: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 21:11:03: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:11:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:11:04: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 21:11:04: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:11:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335514/SRX335514.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling