Job ID = 6456309 SRX = SRX335511 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:32:35 prefetch.2.10.7: 1) Downloading 'SRR952853'... 2020-06-21T10:32:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:38:27 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:38:27 prefetch.2.10.7: 1) 'SRR952853' was downloaded successfully Read 31886596 spots for SRR952853/SRR952853.sra Written 31886596 spots for SRR952853/SRR952853.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:05 31886596 reads; of these: 31886596 (100.00%) were unpaired; of these: 2902511 (9.10%) aligned 0 times 22960084 (72.01%) aligned exactly 1 time 6024001 (18.89%) aligned >1 times 90.90% overall alignment rate Time searching: 00:08:05 Overall time: 00:08:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7862426 / 28984085 = 0.2713 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:54:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:54:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:54:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:54:11: 1000000 INFO @ Sun, 21 Jun 2020 19:54:18: 2000000 INFO @ Sun, 21 Jun 2020 19:54:24: 3000000 INFO @ Sun, 21 Jun 2020 19:54:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:54:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:54:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:54:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:54:37: 5000000 INFO @ Sun, 21 Jun 2020 19:54:41: 1000000 INFO @ Sun, 21 Jun 2020 19:54:44: 6000000 INFO @ Sun, 21 Jun 2020 19:54:48: 2000000 INFO @ Sun, 21 Jun 2020 19:54:51: 7000000 INFO @ Sun, 21 Jun 2020 19:54:55: 3000000 INFO @ Sun, 21 Jun 2020 19:54:58: 8000000 INFO @ Sun, 21 Jun 2020 19:55:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:55:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:55:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:55:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:55:05: 9000000 INFO @ Sun, 21 Jun 2020 19:55:09: 5000000 INFO @ Sun, 21 Jun 2020 19:55:11: 1000000 INFO @ Sun, 21 Jun 2020 19:55:12: 10000000 INFO @ Sun, 21 Jun 2020 19:55:16: 6000000 INFO @ Sun, 21 Jun 2020 19:55:17: 2000000 INFO @ Sun, 21 Jun 2020 19:55:20: 11000000 INFO @ Sun, 21 Jun 2020 19:55:23: 7000000 INFO @ Sun, 21 Jun 2020 19:55:23: 3000000 INFO @ Sun, 21 Jun 2020 19:55:27: 12000000 INFO @ Sun, 21 Jun 2020 19:55:30: 4000000 INFO @ Sun, 21 Jun 2020 19:55:30: 8000000 INFO @ Sun, 21 Jun 2020 19:55:33: 13000000 INFO @ Sun, 21 Jun 2020 19:55:36: 5000000 INFO @ Sun, 21 Jun 2020 19:55:37: 9000000 INFO @ Sun, 21 Jun 2020 19:55:40: 14000000 INFO @ Sun, 21 Jun 2020 19:55:42: 6000000 INFO @ Sun, 21 Jun 2020 19:55:44: 10000000 INFO @ Sun, 21 Jun 2020 19:55:47: 15000000 INFO @ Sun, 21 Jun 2020 19:55:48: 7000000 INFO @ Sun, 21 Jun 2020 19:55:51: 11000000 INFO @ Sun, 21 Jun 2020 19:55:54: 16000000 INFO @ Sun, 21 Jun 2020 19:55:55: 8000000 INFO @ Sun, 21 Jun 2020 19:55:58: 12000000 INFO @ Sun, 21 Jun 2020 19:56:01: 9000000 INFO @ Sun, 21 Jun 2020 19:56:01: 17000000 INFO @ Sun, 21 Jun 2020 19:56:05: 13000000 INFO @ Sun, 21 Jun 2020 19:56:07: 10000000 INFO @ Sun, 21 Jun 2020 19:56:08: 18000000 INFO @ Sun, 21 Jun 2020 19:56:12: 14000000 INFO @ Sun, 21 Jun 2020 19:56:13: 11000000 INFO @ Sun, 21 Jun 2020 19:56:15: 19000000 INFO @ Sun, 21 Jun 2020 19:56:18: 15000000 INFO @ Sun, 21 Jun 2020 19:56:19: 12000000 INFO @ Sun, 21 Jun 2020 19:56:22: 20000000 INFO @ Sun, 21 Jun 2020 19:56:25: 16000000 INFO @ Sun, 21 Jun 2020 19:56:25: 13000000 INFO @ Sun, 21 Jun 2020 19:56:29: 21000000 INFO @ Sun, 21 Jun 2020 19:56:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:56:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:56:30: #1 total tags in treatment: 21121659 INFO @ Sun, 21 Jun 2020 19:56:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:56:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:56:30: #1 tags after filtering in treatment: 21121581 INFO @ Sun, 21 Jun 2020 19:56:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:56:30: #1 finished! INFO @ Sun, 21 Jun 2020 19:56:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:56:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:56:31: 14000000 INFO @ Sun, 21 Jun 2020 19:56:32: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:56:32: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:56:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:56:32: 17000000 INFO @ Sun, 21 Jun 2020 19:56:37: 15000000 INFO @ Sun, 21 Jun 2020 19:56:39: 18000000 INFO @ Sun, 21 Jun 2020 19:56:43: 16000000 INFO @ Sun, 21 Jun 2020 19:56:45: 19000000 INFO @ Sun, 21 Jun 2020 19:56:49: 17000000 INFO @ Sun, 21 Jun 2020 19:56:52: 20000000 INFO @ Sun, 21 Jun 2020 19:56:56: 18000000 INFO @ Sun, 21 Jun 2020 19:56:59: 21000000 INFO @ Sun, 21 Jun 2020 19:57:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:57:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:57:00: #1 total tags in treatment: 21121659 INFO @ Sun, 21 Jun 2020 19:57:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:57:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:57:00: #1 tags after filtering in treatment: 21121581 INFO @ Sun, 21 Jun 2020 19:57:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:57:00: #1 finished! INFO @ Sun, 21 Jun 2020 19:57:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:57:00: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:57:01: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:57:01: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:57:01: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 19:57:02: 19000000 cut: /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:57:07: 20000000 INFO @ Sun, 21 Jun 2020 19:57:13: 21000000 INFO @ Sun, 21 Jun 2020 19:57:14: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:57:14: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:57:14: #1 total tags in treatment: 21121659 INFO @ Sun, 21 Jun 2020 19:57:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:57:14: #1 tags after filtering in treatment: 21121581 INFO @ Sun, 21 Jun 2020 19:57:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:57:14: #1 finished! INFO @ Sun, 21 Jun 2020 19:57:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:57:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:57:16: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:57:16: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:57:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335511/SRX335511.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。