Job ID = 6456308 SRX = SRX335510 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:44:23 prefetch.2.10.7: 1) Downloading 'SRR952852'... 2020-06-21T10:44:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:48:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:48:54 prefetch.2.10.7: 1) 'SRR952852' was downloaded successfully Read 32203362 spots for SRR952852/SRR952852.sra Written 32203362 spots for SRR952852/SRR952852.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:40 32203362 reads; of these: 32203362 (100.00%) were unpaired; of these: 3233838 (10.04%) aligned 0 times 22565346 (70.07%) aligned exactly 1 time 6404178 (19.89%) aligned >1 times 89.96% overall alignment rate Time searching: 00:08:41 Overall time: 00:08:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7793308 / 28969524 = 0.2690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:06:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:06:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:06:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:06:23: 1000000 INFO @ Sun, 21 Jun 2020 20:06:28: 2000000 INFO @ Sun, 21 Jun 2020 20:06:33: 3000000 INFO @ Sun, 21 Jun 2020 20:06:39: 4000000 INFO @ Sun, 21 Jun 2020 20:06:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:06:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:06:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:06:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:06:49: 6000000 INFO @ Sun, 21 Jun 2020 20:06:53: 1000000 INFO @ Sun, 21 Jun 2020 20:06:54: 7000000 INFO @ Sun, 21 Jun 2020 20:06:58: 2000000 INFO @ Sun, 21 Jun 2020 20:07:00: 8000000 INFO @ Sun, 21 Jun 2020 20:07:03: 3000000 INFO @ Sun, 21 Jun 2020 20:07:05: 9000000 INFO @ Sun, 21 Jun 2020 20:07:09: 4000000 INFO @ Sun, 21 Jun 2020 20:07:10: 10000000 INFO @ Sun, 21 Jun 2020 20:07:14: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:07:16: 11000000 INFO @ Sun, 21 Jun 2020 20:07:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:07:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:07:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:07:19: 6000000 INFO @ Sun, 21 Jun 2020 20:07:21: 12000000 INFO @ Sun, 21 Jun 2020 20:07:23: 1000000 INFO @ Sun, 21 Jun 2020 20:07:24: 7000000 INFO @ Sun, 21 Jun 2020 20:07:26: 13000000 INFO @ Sun, 21 Jun 2020 20:07:28: 2000000 INFO @ Sun, 21 Jun 2020 20:07:30: 8000000 INFO @ Sun, 21 Jun 2020 20:07:32: 14000000 INFO @ Sun, 21 Jun 2020 20:07:34: 3000000 INFO @ Sun, 21 Jun 2020 20:07:35: 9000000 INFO @ Sun, 21 Jun 2020 20:07:37: 15000000 INFO @ Sun, 21 Jun 2020 20:07:39: 4000000 INFO @ Sun, 21 Jun 2020 20:07:40: 10000000 INFO @ Sun, 21 Jun 2020 20:07:42: 16000000 INFO @ Sun, 21 Jun 2020 20:07:45: 5000000 INFO @ Sun, 21 Jun 2020 20:07:46: 11000000 INFO @ Sun, 21 Jun 2020 20:07:48: 17000000 INFO @ Sun, 21 Jun 2020 20:07:50: 6000000 INFO @ Sun, 21 Jun 2020 20:07:51: 12000000 INFO @ Sun, 21 Jun 2020 20:07:53: 18000000 INFO @ Sun, 21 Jun 2020 20:07:55: 7000000 INFO @ Sun, 21 Jun 2020 20:07:56: 13000000 INFO @ Sun, 21 Jun 2020 20:07:58: 19000000 INFO @ Sun, 21 Jun 2020 20:08:00: 8000000 INFO @ Sun, 21 Jun 2020 20:08:01: 14000000 INFO @ Sun, 21 Jun 2020 20:08:04: 20000000 INFO @ Sun, 21 Jun 2020 20:08:06: 9000000 INFO @ Sun, 21 Jun 2020 20:08:07: 15000000 INFO @ Sun, 21 Jun 2020 20:08:09: 21000000 INFO @ Sun, 21 Jun 2020 20:08:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:08:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:08:10: #1 total tags in treatment: 21176216 INFO @ Sun, 21 Jun 2020 20:08:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:11: #1 tags after filtering in treatment: 21176163 INFO @ Sun, 21 Jun 2020 20:08:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:11: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:11: 10000000 INFO @ Sun, 21 Jun 2020 20:08:12: 16000000 INFO @ Sun, 21 Jun 2020 20:08:12: #2 number of paired peaks: 66 WARNING @ Sun, 21 Jun 2020 20:08:12: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:08:16: 11000000 INFO @ Sun, 21 Jun 2020 20:08:17: 17000000 INFO @ Sun, 21 Jun 2020 20:08:21: 12000000 INFO @ Sun, 21 Jun 2020 20:08:23: 18000000 INFO @ Sun, 21 Jun 2020 20:08:27: 13000000 INFO @ Sun, 21 Jun 2020 20:08:28: 19000000 INFO @ Sun, 21 Jun 2020 20:08:32: 14000000 INFO @ Sun, 21 Jun 2020 20:08:33: 20000000 INFO @ Sun, 21 Jun 2020 20:08:37: 15000000 INFO @ Sun, 21 Jun 2020 20:08:39: 21000000 INFO @ Sun, 21 Jun 2020 20:08:40: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:08:40: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:08:40: #1 total tags in treatment: 21176216 INFO @ Sun, 21 Jun 2020 20:08:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:41: #1 tags after filtering in treatment: 21176163 INFO @ Sun, 21 Jun 2020 20:08:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:41: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:42: #2 number of paired peaks: 66 WARNING @ Sun, 21 Jun 2020 20:08:42: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:08:43: 16000000 INFO @ Sun, 21 Jun 2020 20:08:48: 17000000 INFO @ Sun, 21 Jun 2020 20:08:53: 18000000 INFO @ Sun, 21 Jun 2020 20:08:59: 19000000 INFO @ Sun, 21 Jun 2020 20:09:04: 20000000 INFO @ Sun, 21 Jun 2020 20:09:09: 21000000 INFO @ Sun, 21 Jun 2020 20:09:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:09:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:09:10: #1 total tags in treatment: 21176216 INFO @ Sun, 21 Jun 2020 20:09:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:09:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:09:11: #1 tags after filtering in treatment: 21176163 INFO @ Sun, 21 Jun 2020 20:09:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:09:11: #1 finished! INFO @ Sun, 21 Jun 2020 20:09:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:09:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:09:13: #2 number of paired peaks: 66 WARNING @ Sun, 21 Jun 2020 20:09:13: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:09:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335510/SRX335510.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。