Job ID = 6456307 SRX = SRX335509 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:38:14 prefetch.2.10.7: 1) Downloading 'SRR952851'... 2020-06-21T10:38:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:48:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:48:19 prefetch.2.10.7: 1) 'SRR952851' was downloaded successfully Read 56147132 spots for SRR952851/SRR952851.sra Written 56147132 spots for SRR952851/SRR952851.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:55 56147132 reads; of these: 56147132 (100.00%) were unpaired; of these: 12280170 (21.87%) aligned 0 times 36904032 (65.73%) aligned exactly 1 time 6962930 (12.40%) aligned >1 times 78.13% overall alignment rate Time searching: 00:11:55 Overall time: 00:11:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 17120721 / 43866962 = 0.3903 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:56: 1000000 INFO @ Sun, 21 Jun 2020 20:12:03: 2000000 INFO @ Sun, 21 Jun 2020 20:12:10: 3000000 INFO @ Sun, 21 Jun 2020 20:12:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:12:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:12:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:12:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:23: 5000000 INFO @ Sun, 21 Jun 2020 20:12:26: 1000000 INFO @ Sun, 21 Jun 2020 20:12:29: 6000000 INFO @ Sun, 21 Jun 2020 20:12:32: 2000000 INFO @ Sun, 21 Jun 2020 20:12:36: 7000000 INFO @ Sun, 21 Jun 2020 20:12:38: 3000000 INFO @ Sun, 21 Jun 2020 20:12:43: 8000000 INFO @ Sun, 21 Jun 2020 20:12:44: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:12:49: 9000000 INFO @ Sun, 21 Jun 2020 20:12:50: 5000000 INFO @ Sun, 21 Jun 2020 20:12:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:12:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:12:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:55: 10000000 INFO @ Sun, 21 Jun 2020 20:12:56: 6000000 INFO @ Sun, 21 Jun 2020 20:12:56: 1000000 INFO @ Sun, 21 Jun 2020 20:13:02: 7000000 INFO @ Sun, 21 Jun 2020 20:13:02: 11000000 INFO @ Sun, 21 Jun 2020 20:13:03: 2000000 INFO @ Sun, 21 Jun 2020 20:13:08: 8000000 INFO @ Sun, 21 Jun 2020 20:13:09: 12000000 INFO @ Sun, 21 Jun 2020 20:13:09: 3000000 INFO @ Sun, 21 Jun 2020 20:13:14: 9000000 INFO @ Sun, 21 Jun 2020 20:13:15: 4000000 INFO @ Sun, 21 Jun 2020 20:13:16: 13000000 INFO @ Sun, 21 Jun 2020 20:13:20: 10000000 INFO @ Sun, 21 Jun 2020 20:13:21: 5000000 INFO @ Sun, 21 Jun 2020 20:13:22: 14000000 INFO @ Sun, 21 Jun 2020 20:13:25: 11000000 INFO @ Sun, 21 Jun 2020 20:13:27: 6000000 INFO @ Sun, 21 Jun 2020 20:13:29: 15000000 INFO @ Sun, 21 Jun 2020 20:13:32: 12000000 INFO @ Sun, 21 Jun 2020 20:13:33: 7000000 INFO @ Sun, 21 Jun 2020 20:13:36: 16000000 INFO @ Sun, 21 Jun 2020 20:13:38: 13000000 INFO @ Sun, 21 Jun 2020 20:13:39: 8000000 INFO @ Sun, 21 Jun 2020 20:13:42: 17000000 INFO @ Sun, 21 Jun 2020 20:13:44: 14000000 INFO @ Sun, 21 Jun 2020 20:13:45: 9000000 INFO @ Sun, 21 Jun 2020 20:13:49: 18000000 INFO @ Sun, 21 Jun 2020 20:13:50: 15000000 INFO @ Sun, 21 Jun 2020 20:13:51: 10000000 INFO @ Sun, 21 Jun 2020 20:13:56: 16000000 INFO @ Sun, 21 Jun 2020 20:13:56: 19000000 INFO @ Sun, 21 Jun 2020 20:13:56: 11000000 INFO @ Sun, 21 Jun 2020 20:14:02: 17000000 INFO @ Sun, 21 Jun 2020 20:14:03: 12000000 INFO @ Sun, 21 Jun 2020 20:14:03: 20000000 INFO @ Sun, 21 Jun 2020 20:14:08: 18000000 INFO @ Sun, 21 Jun 2020 20:14:09: 13000000 INFO @ Sun, 21 Jun 2020 20:14:09: 21000000 INFO @ Sun, 21 Jun 2020 20:14:14: 19000000 INFO @ Sun, 21 Jun 2020 20:14:14: 14000000 INFO @ Sun, 21 Jun 2020 20:14:17: 22000000 INFO @ Sun, 21 Jun 2020 20:14:20: 20000000 INFO @ Sun, 21 Jun 2020 20:14:20: 15000000 INFO @ Sun, 21 Jun 2020 20:14:23: 23000000 INFO @ Sun, 21 Jun 2020 20:14:25: 21000000 INFO @ Sun, 21 Jun 2020 20:14:26: 16000000 INFO @ Sun, 21 Jun 2020 20:14:30: 24000000 INFO @ Sun, 21 Jun 2020 20:14:32: 22000000 INFO @ Sun, 21 Jun 2020 20:14:32: 17000000 INFO @ Sun, 21 Jun 2020 20:14:37: 25000000 INFO @ Sun, 21 Jun 2020 20:14:38: 23000000 INFO @ Sun, 21 Jun 2020 20:14:38: 18000000 INFO @ Sun, 21 Jun 2020 20:14:44: 26000000 INFO @ Sun, 21 Jun 2020 20:14:44: 24000000 INFO @ Sun, 21 Jun 2020 20:14:44: 19000000 INFO @ Sun, 21 Jun 2020 20:14:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:14:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:14:49: #1 total tags in treatment: 26746241 INFO @ Sun, 21 Jun 2020 20:14:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:14:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:14:49: 25000000 INFO @ Sun, 21 Jun 2020 20:14:50: #1 tags after filtering in treatment: 26746180 INFO @ Sun, 21 Jun 2020 20:14:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:14:50: #1 finished! INFO @ Sun, 21 Jun 2020 20:14:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:14:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:14:50: 20000000 INFO @ Sun, 21 Jun 2020 20:14:52: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:14:52: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:14:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:14:55: 26000000 INFO @ Sun, 21 Jun 2020 20:14:56: 21000000 INFO @ Sun, 21 Jun 2020 20:15:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:15:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:15:00: #1 total tags in treatment: 26746241 INFO @ Sun, 21 Jun 2020 20:15:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:15:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:15:01: #1 tags after filtering in treatment: 26746180 INFO @ Sun, 21 Jun 2020 20:15:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:15:01: #1 finished! INFO @ Sun, 21 Jun 2020 20:15:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:15:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:15:02: 22000000 INFO @ Sun, 21 Jun 2020 20:15:03: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:15:03: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:15:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:15:08: 23000000 INFO @ Sun, 21 Jun 2020 20:15:13: 24000000 INFO @ Sun, 21 Jun 2020 20:15:19: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:15:25: 26000000 INFO @ Sun, 21 Jun 2020 20:15:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:15:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:15:29: #1 total tags in treatment: 26746241 INFO @ Sun, 21 Jun 2020 20:15:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:15:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:15:30: #1 tags after filtering in treatment: 26746180 INFO @ Sun, 21 Jun 2020 20:15:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:15:30: #1 finished! INFO @ Sun, 21 Jun 2020 20:15:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:15:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:15:32: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:15:32: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:15:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335509/SRX335509.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。