Job ID = 6456306 SRX = SRX335508 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:38:14 prefetch.2.10.7: 1) Downloading 'SRR952850'... 2020-06-21T10:38:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:44:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:44:55 prefetch.2.10.7: 1) 'SRR952850' was downloaded successfully Read 35336269 spots for SRR952850/SRR952850.sra Written 35336269 spots for SRR952850/SRR952850.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:29 35336269 reads; of these: 35336269 (100.00%) were unpaired; of these: 5685769 (16.09%) aligned 0 times 24061158 (68.09%) aligned exactly 1 time 5589342 (15.82%) aligned >1 times 83.91% overall alignment rate Time searching: 00:09:29 Overall time: 00:09:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7162348 / 29650500 = 0.2416 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:30: 1000000 INFO @ Sun, 21 Jun 2020 20:04:36: 2000000 INFO @ Sun, 21 Jun 2020 20:04:42: 3000000 INFO @ Sun, 21 Jun 2020 20:04:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:53: 5000000 INFO @ Sun, 21 Jun 2020 20:04:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:55: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:55: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:59: 6000000 INFO @ Sun, 21 Jun 2020 20:05:02: 1000000 INFO @ Sun, 21 Jun 2020 20:05:05: 7000000 INFO @ Sun, 21 Jun 2020 20:05:09: 2000000 INFO @ Sun, 21 Jun 2020 20:05:11: 8000000 INFO @ Sun, 21 Jun 2020 20:05:15: 3000000 INFO @ Sun, 21 Jun 2020 20:05:17: 9000000 INFO @ Sun, 21 Jun 2020 20:05:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:05:23: 10000000 INFO @ Sun, 21 Jun 2020 20:05:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:05:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:05:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:05:27: 5000000 INFO @ Sun, 21 Jun 2020 20:05:30: 11000000 INFO @ Sun, 21 Jun 2020 20:05:31: 1000000 INFO @ Sun, 21 Jun 2020 20:05:33: 6000000 INFO @ Sun, 21 Jun 2020 20:05:36: 12000000 INFO @ Sun, 21 Jun 2020 20:05:38: 2000000 INFO @ Sun, 21 Jun 2020 20:05:40: 7000000 INFO @ Sun, 21 Jun 2020 20:05:42: 13000000 INFO @ Sun, 21 Jun 2020 20:05:45: 3000000 INFO @ Sun, 21 Jun 2020 20:05:46: 8000000 INFO @ Sun, 21 Jun 2020 20:05:49: 14000000 INFO @ Sun, 21 Jun 2020 20:05:51: 4000000 INFO @ Sun, 21 Jun 2020 20:05:52: 9000000 INFO @ Sun, 21 Jun 2020 20:05:55: 15000000 INFO @ Sun, 21 Jun 2020 20:05:58: 5000000 INFO @ Sun, 21 Jun 2020 20:05:58: 10000000 INFO @ Sun, 21 Jun 2020 20:06:01: 16000000 INFO @ Sun, 21 Jun 2020 20:06:04: 11000000 INFO @ Sun, 21 Jun 2020 20:06:04: 6000000 INFO @ Sun, 21 Jun 2020 20:06:07: 17000000 INFO @ Sun, 21 Jun 2020 20:06:10: 12000000 INFO @ Sun, 21 Jun 2020 20:06:11: 7000000 INFO @ Sun, 21 Jun 2020 20:06:14: 18000000 INFO @ Sun, 21 Jun 2020 20:06:17: 13000000 INFO @ Sun, 21 Jun 2020 20:06:18: 8000000 INFO @ Sun, 21 Jun 2020 20:06:20: 19000000 INFO @ Sun, 21 Jun 2020 20:06:23: 14000000 INFO @ Sun, 21 Jun 2020 20:06:25: 9000000 INFO @ Sun, 21 Jun 2020 20:06:27: 20000000 INFO @ Sun, 21 Jun 2020 20:06:29: 15000000 INFO @ Sun, 21 Jun 2020 20:06:32: 10000000 INFO @ Sun, 21 Jun 2020 20:06:33: 21000000 INFO @ Sun, 21 Jun 2020 20:06:35: 16000000 INFO @ Sun, 21 Jun 2020 20:06:39: 11000000 INFO @ Sun, 21 Jun 2020 20:06:39: 22000000 INFO @ Sun, 21 Jun 2020 20:06:41: 17000000 INFO @ Sun, 21 Jun 2020 20:06:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:06:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:06:43: #1 total tags in treatment: 22488152 INFO @ Sun, 21 Jun 2020 20:06:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:06:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:06:43: #1 tags after filtering in treatment: 22488151 INFO @ Sun, 21 Jun 2020 20:06:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:06:43: #1 finished! INFO @ Sun, 21 Jun 2020 20:06:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:06:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:06:45: #2 number of paired peaks: 16 WARNING @ Sun, 21 Jun 2020 20:06:45: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:06:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:06:46: 12000000 INFO @ Sun, 21 Jun 2020 20:06:47: 18000000 INFO @ Sun, 21 Jun 2020 20:06:53: 13000000 INFO @ Sun, 21 Jun 2020 20:06:54: 19000000 INFO @ Sun, 21 Jun 2020 20:07:00: 14000000 INFO @ Sun, 21 Jun 2020 20:07:00: 20000000 INFO @ Sun, 21 Jun 2020 20:07:06: 21000000 INFO @ Sun, 21 Jun 2020 20:07:06: 15000000 INFO @ Sun, 21 Jun 2020 20:07:13: 22000000 INFO @ Sun, 21 Jun 2020 20:07:13: 16000000 INFO @ Sun, 21 Jun 2020 20:07:16: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:07:16: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:07:16: #1 total tags in treatment: 22488152 INFO @ Sun, 21 Jun 2020 20:07:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:17: #1 tags after filtering in treatment: 22488151 INFO @ Sun, 21 Jun 2020 20:07:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:17: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:17: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:07:18: #2 number of paired peaks: 16 WARNING @ Sun, 21 Jun 2020 20:07:18: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:07:19: 17000000 INFO @ Sun, 21 Jun 2020 20:07:26: 18000000 INFO @ Sun, 21 Jun 2020 20:07:33: 19000000 INFO @ Sun, 21 Jun 2020 20:07:39: 20000000 INFO @ Sun, 21 Jun 2020 20:07:46: 21000000 INFO @ Sun, 21 Jun 2020 20:07:52: 22000000 INFO @ Sun, 21 Jun 2020 20:07:56: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:07:56: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:07:56: #1 total tags in treatment: 22488152 INFO @ Sun, 21 Jun 2020 20:07:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:57: #1 tags after filtering in treatment: 22488151 INFO @ Sun, 21 Jun 2020 20:07:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:57: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:07:58: #2 number of paired peaks: 16 WARNING @ Sun, 21 Jun 2020 20:07:58: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335508/SRX335508.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。