Job ID = 6456303 SRX = SRX335505 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:40:23 prefetch.2.10.7: 1) Downloading 'SRR952847'... 2020-06-21T10:40:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:46:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:46:59 prefetch.2.10.7: 1) 'SRR952847' was downloaded successfully Read 33349222 spots for SRR952847/SRR952847.sra Written 33349222 spots for SRR952847/SRR952847.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:31 33349222 reads; of these: 33349222 (100.00%) were unpaired; of these: 5059496 (15.17%) aligned 0 times 22969696 (68.88%) aligned exactly 1 time 5320030 (15.95%) aligned >1 times 84.83% overall alignment rate Time searching: 00:08:31 Overall time: 00:08:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6269188 / 28289726 = 0.2216 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:44: 1000000 INFO @ Sun, 21 Jun 2020 20:03:49: 2000000 INFO @ Sun, 21 Jun 2020 20:03:54: 3000000 INFO @ Sun, 21 Jun 2020 20:03:58: 4000000 INFO @ Sun, 21 Jun 2020 20:04:03: 5000000 INFO @ Sun, 21 Jun 2020 20:04:08: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:13: 7000000 INFO @ Sun, 21 Jun 2020 20:04:14: 1000000 INFO @ Sun, 21 Jun 2020 20:04:17: 8000000 INFO @ Sun, 21 Jun 2020 20:04:19: 2000000 INFO @ Sun, 21 Jun 2020 20:04:22: 9000000 INFO @ Sun, 21 Jun 2020 20:04:24: 3000000 INFO @ Sun, 21 Jun 2020 20:04:27: 10000000 INFO @ Sun, 21 Jun 2020 20:04:29: 4000000 INFO @ Sun, 21 Jun 2020 20:04:32: 11000000 INFO @ Sun, 21 Jun 2020 20:04:34: 5000000 INFO @ Sun, 21 Jun 2020 20:04:37: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:38: 6000000 INFO @ Sun, 21 Jun 2020 20:04:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:41: 13000000 INFO @ Sun, 21 Jun 2020 20:04:43: 7000000 INFO @ Sun, 21 Jun 2020 20:04:44: 1000000 INFO @ Sun, 21 Jun 2020 20:04:46: 14000000 INFO @ Sun, 21 Jun 2020 20:04:48: 8000000 INFO @ Sun, 21 Jun 2020 20:04:49: 2000000 INFO @ Sun, 21 Jun 2020 20:04:51: 15000000 INFO @ Sun, 21 Jun 2020 20:04:53: 9000000 INFO @ Sun, 21 Jun 2020 20:04:54: 3000000 INFO @ Sun, 21 Jun 2020 20:04:56: 16000000 INFO @ Sun, 21 Jun 2020 20:04:58: 10000000 INFO @ Sun, 21 Jun 2020 20:04:59: 4000000 INFO @ Sun, 21 Jun 2020 20:05:01: 17000000 INFO @ Sun, 21 Jun 2020 20:05:03: 11000000 INFO @ Sun, 21 Jun 2020 20:05:04: 5000000 INFO @ Sun, 21 Jun 2020 20:05:06: 18000000 INFO @ Sun, 21 Jun 2020 20:05:07: 12000000 INFO @ Sun, 21 Jun 2020 20:05:09: 6000000 INFO @ Sun, 21 Jun 2020 20:05:10: 19000000 INFO @ Sun, 21 Jun 2020 20:05:12: 13000000 INFO @ Sun, 21 Jun 2020 20:05:14: 7000000 INFO @ Sun, 21 Jun 2020 20:05:15: 20000000 INFO @ Sun, 21 Jun 2020 20:05:17: 14000000 INFO @ Sun, 21 Jun 2020 20:05:18: 8000000 INFO @ Sun, 21 Jun 2020 20:05:20: 21000000 INFO @ Sun, 21 Jun 2020 20:05:22: 15000000 INFO @ Sun, 21 Jun 2020 20:05:23: 9000000 INFO @ Sun, 21 Jun 2020 20:05:25: 22000000 INFO @ Sun, 21 Jun 2020 20:05:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:05:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:05:25: #1 total tags in treatment: 22020538 INFO @ Sun, 21 Jun 2020 20:05:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:05:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:05:26: #1 tags after filtering in treatment: 22020535 INFO @ Sun, 21 Jun 2020 20:05:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:05:26: #1 finished! INFO @ Sun, 21 Jun 2020 20:05:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:05:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:05:27: 16000000 INFO @ Sun, 21 Jun 2020 20:05:27: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 20:05:27: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:05:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:05:28: 10000000 INFO @ Sun, 21 Jun 2020 20:05:31: 17000000 INFO @ Sun, 21 Jun 2020 20:05:33: 11000000 INFO @ Sun, 21 Jun 2020 20:05:37: 18000000 INFO @ Sun, 21 Jun 2020 20:05:38: 12000000 INFO @ Sun, 21 Jun 2020 20:05:41: 19000000 INFO @ Sun, 21 Jun 2020 20:05:43: 13000000 INFO @ Sun, 21 Jun 2020 20:05:46: 20000000 INFO @ Sun, 21 Jun 2020 20:05:47: 14000000 INFO @ Sun, 21 Jun 2020 20:05:51: 21000000 INFO @ Sun, 21 Jun 2020 20:05:52: 15000000 INFO @ Sun, 21 Jun 2020 20:05:56: 22000000 INFO @ Sun, 21 Jun 2020 20:05:56: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:05:56: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:05:56: #1 total tags in treatment: 22020538 INFO @ Sun, 21 Jun 2020 20:05:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:05:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:05:57: #1 tags after filtering in treatment: 22020535 INFO @ Sun, 21 Jun 2020 20:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:05:57: #1 finished! INFO @ Sun, 21 Jun 2020 20:05:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:05:57: 16000000 INFO @ Sun, 21 Jun 2020 20:05:58: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 20:05:58: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:05:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:06:02: 17000000 INFO @ Sun, 21 Jun 2020 20:06:07: 18000000 INFO @ Sun, 21 Jun 2020 20:06:12: 19000000 INFO @ Sun, 21 Jun 2020 20:06:16: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:06:21: 21000000 INFO @ Sun, 21 Jun 2020 20:06:26: 22000000 INFO @ Sun, 21 Jun 2020 20:06:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:06:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:06:26: #1 total tags in treatment: 22020538 INFO @ Sun, 21 Jun 2020 20:06:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:06:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:06:27: #1 tags after filtering in treatment: 22020535 INFO @ Sun, 21 Jun 2020 20:06:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:06:27: #1 finished! INFO @ Sun, 21 Jun 2020 20:06:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:06:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:06:28: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 20:06:28: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:06:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335505/SRX335505.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。