Job ID = 6456300 SRX = SRX335502 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:43:23 prefetch.2.10.7: 1) Downloading 'SRR952844'... 2020-06-21T10:43:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:49:41 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:49:41 prefetch.2.10.7: 1) 'SRR952844' was downloaded successfully Read 30968303 spots for SRR952844/SRR952844.sra Written 30968303 spots for SRR952844/SRR952844.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:44 30968303 reads; of these: 30968303 (100.00%) were unpaired; of these: 3748812 (12.11%) aligned 0 times 22049152 (71.20%) aligned exactly 1 time 5170339 (16.70%) aligned >1 times 87.89% overall alignment rate Time searching: 00:07:44 Overall time: 00:07:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7290392 / 27219491 = 0.2678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:56: 1000000 INFO @ Sun, 21 Jun 2020 20:05:01: 2000000 INFO @ Sun, 21 Jun 2020 20:05:07: 3000000 INFO @ Sun, 21 Jun 2020 20:05:12: 4000000 INFO @ Sun, 21 Jun 2020 20:05:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:05:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:05:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:05:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:05:22: 6000000 INFO @ Sun, 21 Jun 2020 20:05:26: 1000000 INFO @ Sun, 21 Jun 2020 20:05:28: 7000000 INFO @ Sun, 21 Jun 2020 20:05:32: 2000000 INFO @ Sun, 21 Jun 2020 20:05:33: 8000000 INFO @ Sun, 21 Jun 2020 20:05:37: 3000000 INFO @ Sun, 21 Jun 2020 20:05:39: 9000000 INFO @ Sun, 21 Jun 2020 20:05:42: 4000000 INFO @ Sun, 21 Jun 2020 20:05:45: 10000000 INFO @ Sun, 21 Jun 2020 20:05:48: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:05:50: 11000000 INFO @ Sun, 21 Jun 2020 20:05:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:05:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:05:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:05:53: 6000000 INFO @ Sun, 21 Jun 2020 20:05:56: 12000000 INFO @ Sun, 21 Jun 2020 20:05:57: 1000000 INFO @ Sun, 21 Jun 2020 20:05:58: 7000000 INFO @ Sun, 21 Jun 2020 20:06:01: 13000000 INFO @ Sun, 21 Jun 2020 20:06:03: 2000000 INFO @ Sun, 21 Jun 2020 20:06:04: 8000000 INFO @ Sun, 21 Jun 2020 20:06:07: 14000000 INFO @ Sun, 21 Jun 2020 20:06:08: 3000000 INFO @ Sun, 21 Jun 2020 20:06:09: 9000000 INFO @ Sun, 21 Jun 2020 20:06:12: 15000000 INFO @ Sun, 21 Jun 2020 20:06:14: 4000000 INFO @ Sun, 21 Jun 2020 20:06:15: 10000000 INFO @ Sun, 21 Jun 2020 20:06:17: 16000000 INFO @ Sun, 21 Jun 2020 20:06:19: 5000000 INFO @ Sun, 21 Jun 2020 20:06:20: 11000000 INFO @ Sun, 21 Jun 2020 20:06:23: 17000000 INFO @ Sun, 21 Jun 2020 20:06:24: 6000000 INFO @ Sun, 21 Jun 2020 20:06:26: 12000000 INFO @ Sun, 21 Jun 2020 20:06:29: 18000000 INFO @ Sun, 21 Jun 2020 20:06:30: 7000000 INFO @ Sun, 21 Jun 2020 20:06:31: 13000000 INFO @ Sun, 21 Jun 2020 20:06:34: 19000000 INFO @ Sun, 21 Jun 2020 20:06:35: 8000000 INFO @ Sun, 21 Jun 2020 20:06:36: 14000000 INFO @ Sun, 21 Jun 2020 20:06:39: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:06:39: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:06:39: #1 total tags in treatment: 19929099 INFO @ Sun, 21 Jun 2020 20:06:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:06:40: #1 tags after filtering in treatment: 19929019 INFO @ Sun, 21 Jun 2020 20:06:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:06:40: #1 finished! INFO @ Sun, 21 Jun 2020 20:06:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:06:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:06:41: 9000000 INFO @ Sun, 21 Jun 2020 20:06:41: #2 number of paired peaks: 48 WARNING @ Sun, 21 Jun 2020 20:06:41: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:06:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:06:42: 15000000 INFO @ Sun, 21 Jun 2020 20:06:46: 10000000 INFO @ Sun, 21 Jun 2020 20:06:47: 16000000 INFO @ Sun, 21 Jun 2020 20:06:51: 11000000 INFO @ Sun, 21 Jun 2020 20:06:53: 17000000 INFO @ Sun, 21 Jun 2020 20:06:56: 12000000 INFO @ Sun, 21 Jun 2020 20:06:58: 18000000 INFO @ Sun, 21 Jun 2020 20:07:02: 13000000 INFO @ Sun, 21 Jun 2020 20:07:03: 19000000 INFO @ Sun, 21 Jun 2020 20:07:07: 14000000 INFO @ Sun, 21 Jun 2020 20:07:08: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:07:08: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:07:08: #1 total tags in treatment: 19929099 INFO @ Sun, 21 Jun 2020 20:07:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:09: #1 tags after filtering in treatment: 19929019 INFO @ Sun, 21 Jun 2020 20:07:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:09: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:09: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:07:10: #2 number of paired peaks: 48 WARNING @ Sun, 21 Jun 2020 20:07:10: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:07:12: 15000000 INFO @ Sun, 21 Jun 2020 20:07:17: 16000000 INFO @ Sun, 21 Jun 2020 20:07:22: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:07:27: 18000000 INFO @ Sun, 21 Jun 2020 20:07:32: 19000000 INFO @ Sun, 21 Jun 2020 20:07:37: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:07:37: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:07:37: #1 total tags in treatment: 19929099 INFO @ Sun, 21 Jun 2020 20:07:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:37: #1 tags after filtering in treatment: 19929019 INFO @ Sun, 21 Jun 2020 20:07:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:37: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:07:39: #2 number of paired peaks: 48 WARNING @ Sun, 21 Jun 2020 20:07:39: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335502/SRX335502.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。