Job ID = 6456297 SRX = SRX335499 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:57:16 prefetch.2.10.7: 1) Downloading 'SRR952841'... 2020-06-21T10:57:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:00:48 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:00:48 prefetch.2.10.7: 1) 'SRR952841' was downloaded successfully Read 31586842 spots for SRR952841/SRR952841.sra Written 31586842 spots for SRR952841/SRR952841.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:55 31586842 reads; of these: 31586842 (100.00%) were unpaired; of these: 1523139 (4.82%) aligned 0 times 24360441 (77.12%) aligned exactly 1 time 5703262 (18.06%) aligned >1 times 95.18% overall alignment rate Time searching: 00:08:55 Overall time: 00:08:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7322511 / 30063703 = 0.2436 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:18:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:18:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:18:54: 1000000 INFO @ Sun, 21 Jun 2020 20:19:00: 2000000 INFO @ Sun, 21 Jun 2020 20:19:06: 3000000 INFO @ Sun, 21 Jun 2020 20:19:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:19:18: 5000000 INFO @ Sun, 21 Jun 2020 20:19:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:19:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:19:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:19:24: 6000000 INFO @ Sun, 21 Jun 2020 20:19:25: 1000000 INFO @ Sun, 21 Jun 2020 20:19:31: 7000000 INFO @ Sun, 21 Jun 2020 20:19:31: 2000000 INFO @ Sun, 21 Jun 2020 20:19:38: 8000000 INFO @ Sun, 21 Jun 2020 20:19:38: 3000000 INFO @ Sun, 21 Jun 2020 20:19:45: 9000000 INFO @ Sun, 21 Jun 2020 20:19:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:19:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:19:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:19:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:19:52: 10000000 INFO @ Sun, 21 Jun 2020 20:19:52: 5000000 INFO @ Sun, 21 Jun 2020 20:19:55: 1000000 INFO @ Sun, 21 Jun 2020 20:19:59: 11000000 INFO @ Sun, 21 Jun 2020 20:20:00: 6000000 INFO @ Sun, 21 Jun 2020 20:20:02: 2000000 INFO @ Sun, 21 Jun 2020 20:20:07: 12000000 INFO @ Sun, 21 Jun 2020 20:20:07: 7000000 INFO @ Sun, 21 Jun 2020 20:20:09: 3000000 INFO @ Sun, 21 Jun 2020 20:20:14: 13000000 INFO @ Sun, 21 Jun 2020 20:20:14: 8000000 INFO @ Sun, 21 Jun 2020 20:20:16: 4000000 INFO @ Sun, 21 Jun 2020 20:20:21: 14000000 INFO @ Sun, 21 Jun 2020 20:20:21: 9000000 INFO @ Sun, 21 Jun 2020 20:20:23: 5000000 INFO @ Sun, 21 Jun 2020 20:20:28: 15000000 INFO @ Sun, 21 Jun 2020 20:20:29: 10000000 INFO @ Sun, 21 Jun 2020 20:20:31: 6000000 INFO @ Sun, 21 Jun 2020 20:20:36: 11000000 INFO @ Sun, 21 Jun 2020 20:20:36: 16000000 INFO @ Sun, 21 Jun 2020 20:20:38: 7000000 INFO @ Sun, 21 Jun 2020 20:20:44: 12000000 INFO @ Sun, 21 Jun 2020 20:20:44: 17000000 INFO @ Sun, 21 Jun 2020 20:20:46: 8000000 INFO @ Sun, 21 Jun 2020 20:20:51: 13000000 INFO @ Sun, 21 Jun 2020 20:20:52: 18000000 INFO @ Sun, 21 Jun 2020 20:20:54: 9000000 INFO @ Sun, 21 Jun 2020 20:20:59: 14000000 INFO @ Sun, 21 Jun 2020 20:21:00: 19000000 INFO @ Sun, 21 Jun 2020 20:21:01: 10000000 INFO @ Sun, 21 Jun 2020 20:21:06: 15000000 INFO @ Sun, 21 Jun 2020 20:21:08: 20000000 INFO @ Sun, 21 Jun 2020 20:21:08: 11000000 INFO @ Sun, 21 Jun 2020 20:21:14: 16000000 INFO @ Sun, 21 Jun 2020 20:21:15: 21000000 INFO @ Sun, 21 Jun 2020 20:21:16: 12000000 INFO @ Sun, 21 Jun 2020 20:21:21: 17000000 INFO @ Sun, 21 Jun 2020 20:21:23: 22000000 INFO @ Sun, 21 Jun 2020 20:21:23: 13000000 INFO @ Sun, 21 Jun 2020 20:21:29: 18000000 INFO @ Sun, 21 Jun 2020 20:21:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:21:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:21:29: #1 total tags in treatment: 22741192 INFO @ Sun, 21 Jun 2020 20:21:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:21:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:21:30: #1 tags after filtering in treatment: 22741185 INFO @ Sun, 21 Jun 2020 20:21:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:21:30: #1 finished! INFO @ Sun, 21 Jun 2020 20:21:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:21:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:21:30: 14000000 INFO @ Sun, 21 Jun 2020 20:21:31: #2 number of paired peaks: 2 WARNING @ Sun, 21 Jun 2020 20:21:31: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:21:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:21:36: 19000000 INFO @ Sun, 21 Jun 2020 20:21:37: 15000000 INFO @ Sun, 21 Jun 2020 20:21:44: 20000000 INFO @ Sun, 21 Jun 2020 20:21:44: 16000000 INFO @ Sun, 21 Jun 2020 20:21:51: 21000000 INFO @ Sun, 21 Jun 2020 20:21:51: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:21:58: 22000000 INFO @ Sun, 21 Jun 2020 20:21:58: 18000000 INFO @ Sun, 21 Jun 2020 20:22:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:22:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:22:03: #1 total tags in treatment: 22741192 INFO @ Sun, 21 Jun 2020 20:22:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:22:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:22:04: #1 tags after filtering in treatment: 22741185 INFO @ Sun, 21 Jun 2020 20:22:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:22:04: #1 finished! INFO @ Sun, 21 Jun 2020 20:22:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:22:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:22:06: #2 number of paired peaks: 2 WARNING @ Sun, 21 Jun 2020 20:22:06: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:22:06: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 20:22:06: 19000000 cut: /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:22:12: 20000000 INFO @ Sun, 21 Jun 2020 20:22:18: 21000000 INFO @ Sun, 21 Jun 2020 20:22:24: 22000000 INFO @ Sun, 21 Jun 2020 20:22:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:22:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:22:29: #1 total tags in treatment: 22741192 INFO @ Sun, 21 Jun 2020 20:22:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:22:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:22:30: #1 tags after filtering in treatment: 22741185 INFO @ Sun, 21 Jun 2020 20:22:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:22:30: #1 finished! INFO @ Sun, 21 Jun 2020 20:22:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:22:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:22:31: #2 number of paired peaks: 2 WARNING @ Sun, 21 Jun 2020 20:22:31: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:22:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335499/SRX335499.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。