Job ID = 6456293 SRX = SRX335498 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:54:46 prefetch.2.10.7: 1) Downloading 'SRR952840'... 2020-06-21T10:54:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:01:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:01:55 prefetch.2.10.7: 1) 'SRR952840' was downloaded successfully Read 32960289 spots for SRR952840/SRR952840.sra Written 32960289 spots for SRR952840/SRR952840.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:45 32960289 reads; of these: 32960289 (100.00%) were unpaired; of these: 5005370 (15.19%) aligned 0 times 22717879 (68.92%) aligned exactly 1 time 5237040 (15.89%) aligned >1 times 84.81% overall alignment rate Time searching: 00:08:45 Overall time: 00:08:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6831889 / 27954919 = 0.2444 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:19:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:19:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:19:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:19:30: 1000000 INFO @ Sun, 21 Jun 2020 20:19:35: 2000000 INFO @ Sun, 21 Jun 2020 20:19:41: 3000000 INFO @ Sun, 21 Jun 2020 20:19:46: 4000000 INFO @ Sun, 21 Jun 2020 20:19:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:19:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:19:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:19:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:19:58: 6000000 INFO @ Sun, 21 Jun 2020 20:20:01: 1000000 INFO @ Sun, 21 Jun 2020 20:20:04: 7000000 INFO @ Sun, 21 Jun 2020 20:20:07: 2000000 INFO @ Sun, 21 Jun 2020 20:20:10: 8000000 INFO @ Sun, 21 Jun 2020 20:20:13: 3000000 INFO @ Sun, 21 Jun 2020 20:20:16: 9000000 INFO @ Sun, 21 Jun 2020 20:20:19: 4000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 20:20:22: 10000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:20:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:20:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:20:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:20:25: 5000000 INFO @ Sun, 21 Jun 2020 20:20:28: 11000000 INFO @ Sun, 21 Jun 2020 20:20:31: 1000000 INFO @ Sun, 21 Jun 2020 20:20:32: 6000000 INFO @ Sun, 21 Jun 2020 20:20:35: 12000000 INFO @ Sun, 21 Jun 2020 20:20:38: 7000000 INFO @ Sun, 21 Jun 2020 20:20:38: 2000000 INFO @ Sun, 21 Jun 2020 20:20:41: 13000000 INFO @ Sun, 21 Jun 2020 20:20:45: 8000000 INFO @ Sun, 21 Jun 2020 20:20:46: 3000000 INFO @ Sun, 21 Jun 2020 20:20:48: 14000000 INFO @ Sun, 21 Jun 2020 20:20:52: 9000000 INFO @ Sun, 21 Jun 2020 20:20:53: 4000000 INFO @ Sun, 21 Jun 2020 20:20:54: 15000000 INFO @ Sun, 21 Jun 2020 20:20:59: 10000000 INFO @ Sun, 21 Jun 2020 20:21:00: 5000000 INFO @ Sun, 21 Jun 2020 20:21:01: 16000000 INFO @ Sun, 21 Jun 2020 20:21:05: 11000000 INFO @ Sun, 21 Jun 2020 20:21:07: 6000000 INFO @ Sun, 21 Jun 2020 20:21:07: 17000000 INFO @ Sun, 21 Jun 2020 20:21:12: 12000000 INFO @ Sun, 21 Jun 2020 20:21:14: 7000000 INFO @ Sun, 21 Jun 2020 20:21:14: 18000000 INFO @ Sun, 21 Jun 2020 20:21:19: 13000000 INFO @ Sun, 21 Jun 2020 20:21:20: 8000000 INFO @ Sun, 21 Jun 2020 20:21:21: 19000000 INFO @ Sun, 21 Jun 2020 20:21:25: 14000000 INFO @ Sun, 21 Jun 2020 20:21:27: 20000000 INFO @ Sun, 21 Jun 2020 20:21:28: 9000000 INFO @ Sun, 21 Jun 2020 20:21:32: 15000000 INFO @ Sun, 21 Jun 2020 20:21:33: 21000000 INFO @ Sun, 21 Jun 2020 20:21:34: 10000000 INFO @ Sun, 21 Jun 2020 20:21:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:21:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:21:34: #1 total tags in treatment: 21123030 INFO @ Sun, 21 Jun 2020 20:21:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:21:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:21:35: #1 tags after filtering in treatment: 21123025 INFO @ Sun, 21 Jun 2020 20:21:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:21:35: #1 finished! INFO @ Sun, 21 Jun 2020 20:21:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:21:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:21:37: #2 number of paired peaks: 2 WARNING @ Sun, 21 Jun 2020 20:21:37: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:21:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:21:38: 16000000 INFO @ Sun, 21 Jun 2020 20:21:42: 11000000 INFO @ Sun, 21 Jun 2020 20:21:45: 17000000 INFO @ Sun, 21 Jun 2020 20:21:49: 12000000 INFO @ Sun, 21 Jun 2020 20:21:51: 18000000 INFO @ Sun, 21 Jun 2020 20:21:55: 13000000 INFO @ Sun, 21 Jun 2020 20:21:58: 19000000 INFO @ Sun, 21 Jun 2020 20:22:02: 14000000 INFO @ Sun, 21 Jun 2020 20:22:04: 20000000 INFO @ Sun, 21 Jun 2020 20:22:09: 15000000 INFO @ Sun, 21 Jun 2020 20:22:10: 21000000 INFO @ Sun, 21 Jun 2020 20:22:11: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:22:11: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:22:11: #1 total tags in treatment: 21123030 INFO @ Sun, 21 Jun 2020 20:22:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:22:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:22:12: #1 tags after filtering in treatment: 21123025 INFO @ Sun, 21 Jun 2020 20:22:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:22:12: #1 finished! INFO @ Sun, 21 Jun 2020 20:22:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:22:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:22:13: #2 number of paired peaks: 2 WARNING @ Sun, 21 Jun 2020 20:22:13: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:22:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:22:16: 16000000 INFO @ Sun, 21 Jun 2020 20:22:22: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:22:29: 18000000 INFO @ Sun, 21 Jun 2020 20:22:35: 19000000 INFO @ Sun, 21 Jun 2020 20:22:42: 20000000 INFO @ Sun, 21 Jun 2020 20:22:49: 21000000 INFO @ Sun, 21 Jun 2020 20:22:50: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:22:50: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:22:50: #1 total tags in treatment: 21123030 INFO @ Sun, 21 Jun 2020 20:22:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:22:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:22:51: #1 tags after filtering in treatment: 21123025 INFO @ Sun, 21 Jun 2020 20:22:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:22:51: #1 finished! INFO @ Sun, 21 Jun 2020 20:22:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:22:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:22:52: #2 number of paired peaks: 2 WARNING @ Sun, 21 Jun 2020 20:22:52: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:22:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335498/SRX335498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。