Job ID = 6456292 SRX = SRX335497 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:46:38 prefetch.2.10.7: 1) Downloading 'SRR952839'... 2020-06-21T10:46:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:54:01 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:54:01 prefetch.2.10.7: 1) 'SRR952839' was downloaded successfully Read 41603816 spots for SRR952839/SRR952839.sra Written 41603816 spots for SRR952839/SRR952839.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:29 41603816 reads; of these: 41603816 (100.00%) were unpaired; of these: 5464152 (13.13%) aligned 0 times 30564753 (73.47%) aligned exactly 1 time 5574911 (13.40%) aligned >1 times 86.87% overall alignment rate Time searching: 00:09:29 Overall time: 00:09:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9941838 / 36139664 = 0.2751 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:14:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:14:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:14:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:14:22: 1000000 INFO @ Sun, 21 Jun 2020 20:14:28: 2000000 INFO @ Sun, 21 Jun 2020 20:14:34: 3000000 INFO @ Sun, 21 Jun 2020 20:14:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:14:46: 5000000 INFO @ Sun, 21 Jun 2020 20:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:14:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:14:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:14:52: 6000000 INFO @ Sun, 21 Jun 2020 20:14:56: 1000000 INFO @ Sun, 21 Jun 2020 20:14:58: 7000000 INFO @ Sun, 21 Jun 2020 20:15:03: 2000000 INFO @ Sun, 21 Jun 2020 20:15:05: 8000000 INFO @ Sun, 21 Jun 2020 20:15:09: 3000000 INFO @ Sun, 21 Jun 2020 20:15:11: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:15:16: 4000000 INFO @ Sun, 21 Jun 2020 20:15:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:15:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:15:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:15:18: 10000000 INFO @ Sun, 21 Jun 2020 20:15:23: 5000000 INFO @ Sun, 21 Jun 2020 20:15:24: 1000000 INFO @ Sun, 21 Jun 2020 20:15:25: 11000000 INFO @ Sun, 21 Jun 2020 20:15:30: 6000000 INFO @ Sun, 21 Jun 2020 20:15:32: 2000000 INFO @ Sun, 21 Jun 2020 20:15:33: 12000000 INFO @ Sun, 21 Jun 2020 20:15:38: 7000000 INFO @ Sun, 21 Jun 2020 20:15:40: 13000000 INFO @ Sun, 21 Jun 2020 20:15:40: 3000000 INFO @ Sun, 21 Jun 2020 20:15:45: 8000000 INFO @ Sun, 21 Jun 2020 20:15:47: 14000000 INFO @ Sun, 21 Jun 2020 20:15:48: 4000000 INFO @ Sun, 21 Jun 2020 20:15:53: 9000000 INFO @ Sun, 21 Jun 2020 20:15:55: 15000000 INFO @ Sun, 21 Jun 2020 20:15:56: 5000000 INFO @ Sun, 21 Jun 2020 20:16:00: 10000000 INFO @ Sun, 21 Jun 2020 20:16:02: 16000000 INFO @ Sun, 21 Jun 2020 20:16:03: 6000000 INFO @ Sun, 21 Jun 2020 20:16:07: 11000000 INFO @ Sun, 21 Jun 2020 20:16:09: 17000000 INFO @ Sun, 21 Jun 2020 20:16:11: 7000000 INFO @ Sun, 21 Jun 2020 20:16:14: 12000000 INFO @ Sun, 21 Jun 2020 20:16:16: 18000000 INFO @ Sun, 21 Jun 2020 20:16:18: 8000000 INFO @ Sun, 21 Jun 2020 20:16:22: 13000000 INFO @ Sun, 21 Jun 2020 20:16:24: 19000000 INFO @ Sun, 21 Jun 2020 20:16:26: 9000000 INFO @ Sun, 21 Jun 2020 20:16:29: 14000000 INFO @ Sun, 21 Jun 2020 20:16:31: 20000000 INFO @ Sun, 21 Jun 2020 20:16:33: 10000000 INFO @ Sun, 21 Jun 2020 20:16:36: 15000000 INFO @ Sun, 21 Jun 2020 20:16:38: 21000000 INFO @ Sun, 21 Jun 2020 20:16:41: 11000000 INFO @ Sun, 21 Jun 2020 20:16:43: 16000000 INFO @ Sun, 21 Jun 2020 20:16:45: 22000000 INFO @ Sun, 21 Jun 2020 20:16:48: 12000000 INFO @ Sun, 21 Jun 2020 20:16:50: 17000000 INFO @ Sun, 21 Jun 2020 20:16:53: 23000000 INFO @ Sun, 21 Jun 2020 20:16:56: 13000000 INFO @ Sun, 21 Jun 2020 20:16:57: 18000000 INFO @ Sun, 21 Jun 2020 20:17:00: 24000000 INFO @ Sun, 21 Jun 2020 20:17:03: 14000000 INFO @ Sun, 21 Jun 2020 20:17:05: 19000000 INFO @ Sun, 21 Jun 2020 20:17:07: 25000000 INFO @ Sun, 21 Jun 2020 20:17:11: 15000000 INFO @ Sun, 21 Jun 2020 20:17:12: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:17:14: 26000000 INFO @ Sun, 21 Jun 2020 20:17:16: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:17:16: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:17:16: #1 total tags in treatment: 26197826 INFO @ Sun, 21 Jun 2020 20:17:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:17: #1 tags after filtering in treatment: 26197825 INFO @ Sun, 21 Jun 2020 20:17:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:17: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:18: 16000000 INFO @ Sun, 21 Jun 2020 20:17:18: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:17:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:17:19: 21000000 INFO @ Sun, 21 Jun 2020 20:17:25: 17000000 INFO @ Sun, 21 Jun 2020 20:17:26: 22000000 INFO @ Sun, 21 Jun 2020 20:17:33: 18000000 INFO @ Sun, 21 Jun 2020 20:17:33: 23000000 INFO @ Sun, 21 Jun 2020 20:17:40: 24000000 INFO @ Sun, 21 Jun 2020 20:17:40: 19000000 INFO @ Sun, 21 Jun 2020 20:17:47: 25000000 INFO @ Sun, 21 Jun 2020 20:17:48: 20000000 INFO @ Sun, 21 Jun 2020 20:17:54: 26000000 INFO @ Sun, 21 Jun 2020 20:17:55: 21000000 INFO @ Sun, 21 Jun 2020 20:17:56: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:17:56: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:17:56: #1 total tags in treatment: 26197826 INFO @ Sun, 21 Jun 2020 20:17:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:56: #1 tags after filtering in treatment: 26197825 INFO @ Sun, 21 Jun 2020 20:17:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:56: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:58: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:17:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:18:03: 22000000 INFO @ Sun, 21 Jun 2020 20:18:10: 23000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:18:16: 24000000 INFO @ Sun, 21 Jun 2020 20:18:23: 25000000 INFO @ Sun, 21 Jun 2020 20:18:30: 26000000 INFO @ Sun, 21 Jun 2020 20:18:32: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:18:32: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:18:32: #1 total tags in treatment: 26197826 INFO @ Sun, 21 Jun 2020 20:18:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:18:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:18:32: #1 tags after filtering in treatment: 26197825 INFO @ Sun, 21 Jun 2020 20:18:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:18:32: #1 finished! INFO @ Sun, 21 Jun 2020 20:18:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:18:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:18:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:18:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:18:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335497/SRX335497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling