Job ID = 6456289 SRX = SRX335494 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:07:54 prefetch.2.10.7: 1) Downloading 'SRR952836'... 2020-06-21T11:07:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:13:58 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:13:58 prefetch.2.10.7: 1) 'SRR952836' was downloaded successfully Read 35836077 spots for SRR952836/SRR952836.sra Written 35836077 spots for SRR952836/SRR952836.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:08:30 35836077 reads; of these: 35836077 (100.00%) were unpaired; of these: 3744275 (10.45%) aligned 0 times 27832420 (77.67%) aligned exactly 1 time 4259382 (11.89%) aligned >1 times 89.55% overall alignment rate Time searching: 00:08:31 Overall time: 00:08:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8718556 / 32091802 = 0.2717 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:31:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:31:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:31:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:31:34: 1000000 INFO @ Sun, 21 Jun 2020 20:31:41: 2000000 INFO @ Sun, 21 Jun 2020 20:31:48: 3000000 INFO @ Sun, 21 Jun 2020 20:31:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:31:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:31:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:31:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:32:01: 5000000 INFO @ Sun, 21 Jun 2020 20:32:04: 1000000 INFO @ Sun, 21 Jun 2020 20:32:08: 6000000 INFO @ Sun, 21 Jun 2020 20:32:10: 2000000 INFO @ Sun, 21 Jun 2020 20:32:15: 7000000 INFO @ Sun, 21 Jun 2020 20:32:17: 3000000 INFO @ Sun, 21 Jun 2020 20:32:23: 8000000 INFO @ Sun, 21 Jun 2020 20:32:23: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:32:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:32:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:32:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:32:30: 5000000 INFO @ Sun, 21 Jun 2020 20:32:30: 9000000 INFO @ Sun, 21 Jun 2020 20:32:35: 1000000 INFO @ Sun, 21 Jun 2020 20:32:36: 6000000 INFO @ Sun, 21 Jun 2020 20:32:37: 10000000 INFO @ Sun, 21 Jun 2020 20:32:42: 2000000 INFO @ Sun, 21 Jun 2020 20:32:42: 7000000 INFO @ Sun, 21 Jun 2020 20:32:44: 11000000 INFO @ Sun, 21 Jun 2020 20:32:49: 8000000 INFO @ Sun, 21 Jun 2020 20:32:49: 3000000 INFO @ Sun, 21 Jun 2020 20:32:51: 12000000 INFO @ Sun, 21 Jun 2020 20:32:56: 9000000 INFO @ Sun, 21 Jun 2020 20:32:56: 4000000 INFO @ Sun, 21 Jun 2020 20:32:59: 13000000 INFO @ Sun, 21 Jun 2020 20:33:02: 10000000 INFO @ Sun, 21 Jun 2020 20:33:04: 5000000 INFO @ Sun, 21 Jun 2020 20:33:06: 14000000 INFO @ Sun, 21 Jun 2020 20:33:09: 11000000 INFO @ Sun, 21 Jun 2020 20:33:11: 6000000 INFO @ Sun, 21 Jun 2020 20:33:13: 15000000 INFO @ Sun, 21 Jun 2020 20:33:16: 12000000 INFO @ Sun, 21 Jun 2020 20:33:18: 7000000 INFO @ Sun, 21 Jun 2020 20:33:20: 16000000 INFO @ Sun, 21 Jun 2020 20:33:22: 13000000 INFO @ Sun, 21 Jun 2020 20:33:26: 8000000 INFO @ Sun, 21 Jun 2020 20:33:27: 17000000 INFO @ Sun, 21 Jun 2020 20:33:29: 14000000 INFO @ Sun, 21 Jun 2020 20:33:33: 9000000 INFO @ Sun, 21 Jun 2020 20:33:35: 18000000 INFO @ Sun, 21 Jun 2020 20:33:35: 15000000 INFO @ Sun, 21 Jun 2020 20:33:40: 10000000 INFO @ Sun, 21 Jun 2020 20:33:42: 16000000 INFO @ Sun, 21 Jun 2020 20:33:42: 19000000 INFO @ Sun, 21 Jun 2020 20:33:47: 11000000 INFO @ Sun, 21 Jun 2020 20:33:48: 17000000 INFO @ Sun, 21 Jun 2020 20:33:50: 20000000 INFO @ Sun, 21 Jun 2020 20:33:55: 12000000 INFO @ Sun, 21 Jun 2020 20:33:55: 18000000 INFO @ Sun, 21 Jun 2020 20:33:57: 21000000 INFO @ Sun, 21 Jun 2020 20:34:01: 19000000 INFO @ Sun, 21 Jun 2020 20:34:02: 13000000 INFO @ Sun, 21 Jun 2020 20:34:04: 22000000 INFO @ Sun, 21 Jun 2020 20:34:08: 20000000 INFO @ Sun, 21 Jun 2020 20:34:09: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:34:12: 23000000 INFO @ Sun, 21 Jun 2020 20:34:15: 21000000 INFO @ Sun, 21 Jun 2020 20:34:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:34:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:34:15: #1 total tags in treatment: 23373246 INFO @ Sun, 21 Jun 2020 20:34:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:34:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:34:15: #1 tags after filtering in treatment: 23373239 INFO @ Sun, 21 Jun 2020 20:34:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:34:15: #1 finished! INFO @ Sun, 21 Jun 2020 20:34:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:34:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:34:17: 15000000 INFO @ Sun, 21 Jun 2020 20:34:17: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:34:17: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:34:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:34:21: 22000000 INFO @ Sun, 21 Jun 2020 20:34:24: 16000000 INFO @ Sun, 21 Jun 2020 20:34:28: 23000000 INFO @ Sun, 21 Jun 2020 20:34:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:34:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:34:30: #1 total tags in treatment: 23373246 INFO @ Sun, 21 Jun 2020 20:34:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:34:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:34:31: #1 tags after filtering in treatment: 23373239 INFO @ Sun, 21 Jun 2020 20:34:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:34:31: #1 finished! INFO @ Sun, 21 Jun 2020 20:34:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:34:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:34:31: 17000000 INFO @ Sun, 21 Jun 2020 20:34:32: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:34:32: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:34:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:34:38: 18000000 INFO @ Sun, 21 Jun 2020 20:34:45: 19000000 INFO @ Sun, 21 Jun 2020 20:34:52: 20000000 INFO @ Sun, 21 Jun 2020 20:34:59: 21000000 INFO @ Sun, 21 Jun 2020 20:35:05: 22000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:35:12: 23000000 INFO @ Sun, 21 Jun 2020 20:35:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:35:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:35:15: #1 total tags in treatment: 23373246 INFO @ Sun, 21 Jun 2020 20:35:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:35:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:35:16: #1 tags after filtering in treatment: 23373239 INFO @ Sun, 21 Jun 2020 20:35:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:35:16: #1 finished! INFO @ Sun, 21 Jun 2020 20:35:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:35:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:35:17: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:35:17: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:35:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335494/SRX335494.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling