Job ID = 6456285 SRX = SRX335491 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:56:16 prefetch.2.10.7: 1) Downloading 'SRR952833'... 2020-06-21T10:56:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:02:31 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:02:31 prefetch.2.10.7: 1) 'SRR952833' was downloaded successfully Read 32260958 spots for SRR952833/SRR952833.sra Written 32260958 spots for SRR952833/SRR952833.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:05 32260958 reads; of these: 32260958 (100.00%) were unpaired; of these: 1543948 (4.79%) aligned 0 times 27641194 (85.68%) aligned exactly 1 time 3075816 (9.53%) aligned >1 times 95.21% overall alignment rate Time searching: 00:07:05 Overall time: 00:07:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8350228 / 30717010 = 0.2718 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:17:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:17:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:17:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:17:56: 1000000 INFO @ Sun, 21 Jun 2020 20:18:00: 2000000 INFO @ Sun, 21 Jun 2020 20:18:05: 3000000 INFO @ Sun, 21 Jun 2020 20:18:10: 4000000 INFO @ Sun, 21 Jun 2020 20:18:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:18:20: 6000000 INFO @ Sun, 21 Jun 2020 20:18:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:18:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:18:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:18:25: 7000000 INFO @ Sun, 21 Jun 2020 20:18:26: 1000000 INFO @ Sun, 21 Jun 2020 20:18:30: 8000000 INFO @ Sun, 21 Jun 2020 20:18:31: 2000000 INFO @ Sun, 21 Jun 2020 20:18:35: 9000000 INFO @ Sun, 21 Jun 2020 20:18:36: 3000000 INFO @ Sun, 21 Jun 2020 20:18:40: 10000000 INFO @ Sun, 21 Jun 2020 20:18:41: 4000000 INFO @ Sun, 21 Jun 2020 20:18:45: 11000000 INFO @ Sun, 21 Jun 2020 20:18:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:18:50: 12000000 INFO @ Sun, 21 Jun 2020 20:18:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:18:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:18:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:18:51: 6000000 INFO @ Sun, 21 Jun 2020 20:18:55: 13000000 INFO @ Sun, 21 Jun 2020 20:18:56: 1000000 INFO @ Sun, 21 Jun 2020 20:18:56: 7000000 INFO @ Sun, 21 Jun 2020 20:19:00: 14000000 INFO @ Sun, 21 Jun 2020 20:19:01: 2000000 INFO @ Sun, 21 Jun 2020 20:19:02: 8000000 INFO @ Sun, 21 Jun 2020 20:19:05: 15000000 INFO @ Sun, 21 Jun 2020 20:19:06: 3000000 INFO @ Sun, 21 Jun 2020 20:19:07: 9000000 INFO @ Sun, 21 Jun 2020 20:19:10: 16000000 INFO @ Sun, 21 Jun 2020 20:19:11: 4000000 INFO @ Sun, 21 Jun 2020 20:19:12: 10000000 INFO @ Sun, 21 Jun 2020 20:19:15: 17000000 INFO @ Sun, 21 Jun 2020 20:19:16: 5000000 INFO @ Sun, 21 Jun 2020 20:19:17: 11000000 INFO @ Sun, 21 Jun 2020 20:19:20: 18000000 INFO @ Sun, 21 Jun 2020 20:19:21: 6000000 INFO @ Sun, 21 Jun 2020 20:19:22: 12000000 INFO @ Sun, 21 Jun 2020 20:19:25: 19000000 INFO @ Sun, 21 Jun 2020 20:19:27: 7000000 INFO @ Sun, 21 Jun 2020 20:19:27: 13000000 INFO @ Sun, 21 Jun 2020 20:19:30: 20000000 INFO @ Sun, 21 Jun 2020 20:19:32: 8000000 INFO @ Sun, 21 Jun 2020 20:19:32: 14000000 INFO @ Sun, 21 Jun 2020 20:19:35: 21000000 INFO @ Sun, 21 Jun 2020 20:19:37: 9000000 INFO @ Sun, 21 Jun 2020 20:19:37: 15000000 INFO @ Sun, 21 Jun 2020 20:19:40: 22000000 INFO @ Sun, 21 Jun 2020 20:19:42: 16000000 INFO @ Sun, 21 Jun 2020 20:19:42: 10000000 INFO @ Sun, 21 Jun 2020 20:19:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:19:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:19:43: #1 total tags in treatment: 22366782 INFO @ Sun, 21 Jun 2020 20:19:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:19:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:19:43: #1 tags after filtering in treatment: 22366771 INFO @ Sun, 21 Jun 2020 20:19:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:19:43: #1 finished! INFO @ Sun, 21 Jun 2020 20:19:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:19:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:19:44: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:19:44: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:19:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:19:47: 17000000 INFO @ Sun, 21 Jun 2020 20:19:47: 11000000 INFO @ Sun, 21 Jun 2020 20:19:52: 18000000 INFO @ Sun, 21 Jun 2020 20:19:52: 12000000 INFO @ Sun, 21 Jun 2020 20:19:57: 13000000 INFO @ Sun, 21 Jun 2020 20:19:57: 19000000 INFO @ Sun, 21 Jun 2020 20:20:02: 14000000 INFO @ Sun, 21 Jun 2020 20:20:02: 20000000 INFO @ Sun, 21 Jun 2020 20:20:07: 15000000 INFO @ Sun, 21 Jun 2020 20:20:08: 21000000 INFO @ Sun, 21 Jun 2020 20:20:12: 16000000 INFO @ Sun, 21 Jun 2020 20:20:13: 22000000 INFO @ Sun, 21 Jun 2020 20:20:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:20:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:20:15: #1 total tags in treatment: 22366782 INFO @ Sun, 21 Jun 2020 20:20:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:20:16: #1 tags after filtering in treatment: 22366771 INFO @ Sun, 21 Jun 2020 20:20:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:20:16: #1 finished! INFO @ Sun, 21 Jun 2020 20:20:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:20:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:20:17: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:20:17: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:20:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:20:17: 17000000 INFO @ Sun, 21 Jun 2020 20:20:22: 18000000 INFO @ Sun, 21 Jun 2020 20:20:27: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:20:32: 20000000 INFO @ Sun, 21 Jun 2020 20:20:37: 21000000 INFO @ Sun, 21 Jun 2020 20:20:42: 22000000 INFO @ Sun, 21 Jun 2020 20:20:45: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:20:45: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:20:45: #1 total tags in treatment: 22366782 INFO @ Sun, 21 Jun 2020 20:20:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:20:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:20:45: #1 tags after filtering in treatment: 22366771 INFO @ Sun, 21 Jun 2020 20:20:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:20:45: #1 finished! INFO @ Sun, 21 Jun 2020 20:20:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:20:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:20:47: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 20:20:47: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:20:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335491/SRX335491.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。