Job ID = 6456284 SRX = SRX335490 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:40:08 prefetch.2.10.7: 1) Downloading 'SRR952832'... 2020-06-21T10:40:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:48:28 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:48:28 prefetch.2.10.7: 1) 'SRR952832' was downloaded successfully Read 45697542 spots for SRR952832/SRR952832.sra Written 45697542 spots for SRR952832/SRR952832.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:11:11 45697542 reads; of these: 45697542 (100.00%) were unpaired; of these: 2230504 (4.88%) aligned 0 times 35259623 (77.16%) aligned exactly 1 time 8207415 (17.96%) aligned >1 times 95.12% overall alignment rate Time searching: 00:11:12 Overall time: 00:11:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13740964 / 43467038 = 0.3161 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:34: 1000000 INFO @ Sun, 21 Jun 2020 20:11:40: 2000000 INFO @ Sun, 21 Jun 2020 20:11:45: 3000000 INFO @ Sun, 21 Jun 2020 20:11:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:57: 5000000 INFO @ Sun, 21 Jun 2020 20:11:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:04: 6000000 INFO @ Sun, 21 Jun 2020 20:12:06: 1000000 INFO @ Sun, 21 Jun 2020 20:12:11: 7000000 INFO @ Sun, 21 Jun 2020 20:12:13: 2000000 INFO @ Sun, 21 Jun 2020 20:12:19: 8000000 INFO @ Sun, 21 Jun 2020 20:12:21: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:12:26: 9000000 INFO @ Sun, 21 Jun 2020 20:12:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:12:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:12:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:29: 4000000 INFO @ Sun, 21 Jun 2020 20:12:34: 10000000 INFO @ Sun, 21 Jun 2020 20:12:36: 1000000 INFO @ Sun, 21 Jun 2020 20:12:37: 5000000 INFO @ Sun, 21 Jun 2020 20:12:41: 11000000 INFO @ Sun, 21 Jun 2020 20:12:43: 2000000 INFO @ Sun, 21 Jun 2020 20:12:44: 6000000 INFO @ Sun, 21 Jun 2020 20:12:49: 12000000 INFO @ Sun, 21 Jun 2020 20:12:51: 3000000 INFO @ Sun, 21 Jun 2020 20:12:52: 7000000 INFO @ Sun, 21 Jun 2020 20:12:57: 13000000 INFO @ Sun, 21 Jun 2020 20:12:59: 4000000 INFO @ Sun, 21 Jun 2020 20:13:00: 8000000 INFO @ Sun, 21 Jun 2020 20:13:04: 14000000 INFO @ Sun, 21 Jun 2020 20:13:06: 5000000 INFO @ Sun, 21 Jun 2020 20:13:08: 9000000 INFO @ Sun, 21 Jun 2020 20:13:12: 15000000 INFO @ Sun, 21 Jun 2020 20:13:14: 6000000 INFO @ Sun, 21 Jun 2020 20:13:16: 10000000 INFO @ Sun, 21 Jun 2020 20:13:19: 16000000 INFO @ Sun, 21 Jun 2020 20:13:22: 7000000 INFO @ Sun, 21 Jun 2020 20:13:24: 11000000 INFO @ Sun, 21 Jun 2020 20:13:27: 17000000 INFO @ Sun, 21 Jun 2020 20:13:29: 8000000 INFO @ Sun, 21 Jun 2020 20:13:32: 12000000 INFO @ Sun, 21 Jun 2020 20:13:35: 18000000 INFO @ Sun, 21 Jun 2020 20:13:37: 9000000 INFO @ Sun, 21 Jun 2020 20:13:39: 13000000 INFO @ Sun, 21 Jun 2020 20:13:42: 19000000 INFO @ Sun, 21 Jun 2020 20:13:44: 10000000 INFO @ Sun, 21 Jun 2020 20:13:47: 14000000 INFO @ Sun, 21 Jun 2020 20:13:50: 20000000 INFO @ Sun, 21 Jun 2020 20:13:52: 11000000 INFO @ Sun, 21 Jun 2020 20:13:55: 15000000 INFO @ Sun, 21 Jun 2020 20:13:57: 21000000 INFO @ Sun, 21 Jun 2020 20:14:00: 12000000 INFO @ Sun, 21 Jun 2020 20:14:03: 16000000 INFO @ Sun, 21 Jun 2020 20:14:05: 22000000 INFO @ Sun, 21 Jun 2020 20:14:07: 13000000 INFO @ Sun, 21 Jun 2020 20:14:12: 17000000 INFO @ Sun, 21 Jun 2020 20:14:12: 23000000 INFO @ Sun, 21 Jun 2020 20:14:15: 14000000 INFO @ Sun, 21 Jun 2020 20:14:20: 18000000 INFO @ Sun, 21 Jun 2020 20:14:20: 24000000 INFO @ Sun, 21 Jun 2020 20:14:23: 15000000 INFO @ Sun, 21 Jun 2020 20:14:27: 19000000 INFO @ Sun, 21 Jun 2020 20:14:28: 25000000 INFO @ Sun, 21 Jun 2020 20:14:30: 16000000 INFO @ Sun, 21 Jun 2020 20:14:35: 26000000 INFO @ Sun, 21 Jun 2020 20:14:35: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:14:38: 17000000 INFO @ Sun, 21 Jun 2020 20:14:43: 27000000 INFO @ Sun, 21 Jun 2020 20:14:43: 21000000 INFO @ Sun, 21 Jun 2020 20:14:45: 18000000 INFO @ Sun, 21 Jun 2020 20:14:50: 28000000 INFO @ Sun, 21 Jun 2020 20:14:51: 22000000 INFO @ Sun, 21 Jun 2020 20:14:53: 19000000 INFO @ Sun, 21 Jun 2020 20:14:58: 29000000 INFO @ Sun, 21 Jun 2020 20:14:59: 23000000 INFO @ Sun, 21 Jun 2020 20:15:01: 20000000 INFO @ Sun, 21 Jun 2020 20:15:04: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:15:04: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:15:04: #1 total tags in treatment: 29726074 INFO @ Sun, 21 Jun 2020 20:15:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:15:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:15:04: #1 tags after filtering in treatment: 29726073 INFO @ Sun, 21 Jun 2020 20:15:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:15:04: #1 finished! INFO @ Sun, 21 Jun 2020 20:15:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:15:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:15:06: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:15:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:15:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:15:07: 24000000 INFO @ Sun, 21 Jun 2020 20:15:09: 21000000 INFO @ Sun, 21 Jun 2020 20:15:15: 25000000 INFO @ Sun, 21 Jun 2020 20:15:16: 22000000 INFO @ Sun, 21 Jun 2020 20:15:22: 26000000 INFO @ Sun, 21 Jun 2020 20:15:24: 23000000 INFO @ Sun, 21 Jun 2020 20:15:30: 27000000 INFO @ Sun, 21 Jun 2020 20:15:32: 24000000 INFO @ Sun, 21 Jun 2020 20:15:38: 28000000 INFO @ Sun, 21 Jun 2020 20:15:41: 25000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:15:45: 29000000 INFO @ Sun, 21 Jun 2020 20:15:49: 26000000 INFO @ Sun, 21 Jun 2020 20:15:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:15:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:15:51: #1 total tags in treatment: 29726074 INFO @ Sun, 21 Jun 2020 20:15:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:15:52: #1 tags after filtering in treatment: 29726073 INFO @ Sun, 21 Jun 2020 20:15:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:15:52: #1 finished! INFO @ Sun, 21 Jun 2020 20:15:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:15:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:15:54: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:15:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:15:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:15:56: 27000000 INFO @ Sun, 21 Jun 2020 20:16:04: 28000000 INFO @ Sun, 21 Jun 2020 20:16:11: 29000000 INFO @ Sun, 21 Jun 2020 20:16:17: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:16:17: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:16:17: #1 total tags in treatment: 29726074 INFO @ Sun, 21 Jun 2020 20:16:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:16:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:16:17: #1 tags after filtering in treatment: 29726073 INFO @ Sun, 21 Jun 2020 20:16:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:16:17: #1 finished! INFO @ Sun, 21 Jun 2020 20:16:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:16:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:16:19: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:16:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:16:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335490/SRX335490.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling