Job ID = 6456283 SRX = SRX335489 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:37:59 prefetch.2.10.7: 1) Downloading 'SRR952831'... 2020-06-21T10:37:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:46:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:46:44 prefetch.2.10.7: 1) 'SRR952831' was downloaded successfully Read 47571085 spots for SRR952831/SRR952831.sra Written 47571085 spots for SRR952831/SRR952831.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:13 47571085 reads; of these: 47571085 (100.00%) were unpaired; of these: 2504750 (5.27%) aligned 0 times 40297506 (84.71%) aligned exactly 1 time 4768829 (10.02%) aligned >1 times 94.73% overall alignment rate Time searching: 00:11:13 Overall time: 00:11:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 16753212 / 45066335 = 0.3717 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:10:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:10:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:10:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:10:06: 1000000 INFO @ Sun, 21 Jun 2020 20:10:12: 2000000 INFO @ Sun, 21 Jun 2020 20:10:17: 3000000 INFO @ Sun, 21 Jun 2020 20:10:23: 4000000 INFO @ Sun, 21 Jun 2020 20:10:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:10:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:10:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:10:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:10:34: 6000000 INFO @ Sun, 21 Jun 2020 20:10:37: 1000000 INFO @ Sun, 21 Jun 2020 20:10:40: 7000000 INFO @ Sun, 21 Jun 2020 20:10:45: 2000000 INFO @ Sun, 21 Jun 2020 20:10:46: 8000000 INFO @ Sun, 21 Jun 2020 20:10:51: 3000000 INFO @ Sun, 21 Jun 2020 20:10:52: 9000000 INFO @ Sun, 21 Jun 2020 20:10:58: 4000000 INFO @ Sun, 21 Jun 2020 20:10:58: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:04: 11000000 INFO @ Sun, 21 Jun 2020 20:11:05: 5000000 INFO @ Sun, 21 Jun 2020 20:11:07: 1000000 INFO @ Sun, 21 Jun 2020 20:11:11: 12000000 INFO @ Sun, 21 Jun 2020 20:11:12: 6000000 INFO @ Sun, 21 Jun 2020 20:11:13: 2000000 INFO @ Sun, 21 Jun 2020 20:11:17: 13000000 INFO @ Sun, 21 Jun 2020 20:11:19: 7000000 INFO @ Sun, 21 Jun 2020 20:11:19: 3000000 INFO @ Sun, 21 Jun 2020 20:11:24: 14000000 INFO @ Sun, 21 Jun 2020 20:11:26: 8000000 INFO @ Sun, 21 Jun 2020 20:11:26: 4000000 INFO @ Sun, 21 Jun 2020 20:11:30: 15000000 INFO @ Sun, 21 Jun 2020 20:11:33: 5000000 INFO @ Sun, 21 Jun 2020 20:11:33: 9000000 INFO @ Sun, 21 Jun 2020 20:11:37: 16000000 INFO @ Sun, 21 Jun 2020 20:11:39: 6000000 INFO @ Sun, 21 Jun 2020 20:11:40: 10000000 INFO @ Sun, 21 Jun 2020 20:11:43: 17000000 INFO @ Sun, 21 Jun 2020 20:11:46: 7000000 INFO @ Sun, 21 Jun 2020 20:11:47: 11000000 INFO @ Sun, 21 Jun 2020 20:11:49: 18000000 INFO @ Sun, 21 Jun 2020 20:11:52: 8000000 INFO @ Sun, 21 Jun 2020 20:11:54: 12000000 INFO @ Sun, 21 Jun 2020 20:11:56: 19000000 INFO @ Sun, 21 Jun 2020 20:11:59: 9000000 INFO @ Sun, 21 Jun 2020 20:12:01: 13000000 INFO @ Sun, 21 Jun 2020 20:12:02: 20000000 INFO @ Sun, 21 Jun 2020 20:12:05: 10000000 INFO @ Sun, 21 Jun 2020 20:12:08: 14000000 INFO @ Sun, 21 Jun 2020 20:12:09: 21000000 INFO @ Sun, 21 Jun 2020 20:12:12: 11000000 INFO @ Sun, 21 Jun 2020 20:12:15: 15000000 INFO @ Sun, 21 Jun 2020 20:12:15: 22000000 INFO @ Sun, 21 Jun 2020 20:12:18: 12000000 INFO @ Sun, 21 Jun 2020 20:12:22: 16000000 INFO @ Sun, 21 Jun 2020 20:12:22: 23000000 INFO @ Sun, 21 Jun 2020 20:12:24: 13000000 INFO @ Sun, 21 Jun 2020 20:12:29: 24000000 INFO @ Sun, 21 Jun 2020 20:12:29: 17000000 INFO @ Sun, 21 Jun 2020 20:12:31: 14000000 INFO @ Sun, 21 Jun 2020 20:12:35: 25000000 INFO @ Sun, 21 Jun 2020 20:12:36: 18000000 INFO @ Sun, 21 Jun 2020 20:12:37: 15000000 INFO @ Sun, 21 Jun 2020 20:12:41: 26000000 INFO @ Sun, 21 Jun 2020 20:12:43: 19000000 INFO @ Sun, 21 Jun 2020 20:12:43: 16000000 INFO @ Sun, 21 Jun 2020 20:12:48: 27000000 INFO @ Sun, 21 Jun 2020 20:12:50: 17000000 INFO @ Sun, 21 Jun 2020 20:12:50: 20000000 INFO @ Sun, 21 Jun 2020 20:12:54: 28000000 INFO @ Sun, 21 Jun 2020 20:12:56: 18000000 INFO @ Sun, 21 Jun 2020 20:12:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:12:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:12:57: #1 total tags in treatment: 28313123 INFO @ Sun, 21 Jun 2020 20:12:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:12:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:12:57: 21000000 INFO @ Sun, 21 Jun 2020 20:12:57: #1 tags after filtering in treatment: 28313122 INFO @ Sun, 21 Jun 2020 20:12:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:12:57: #1 finished! INFO @ Sun, 21 Jun 2020 20:12:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:12:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:12:59: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:12:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:12:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:13:02: 19000000 INFO @ Sun, 21 Jun 2020 20:13:04: 22000000 INFO @ Sun, 21 Jun 2020 20:13:08: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:13:11: 23000000 INFO @ Sun, 21 Jun 2020 20:13:15: 21000000 INFO @ Sun, 21 Jun 2020 20:13:18: 24000000 INFO @ Sun, 21 Jun 2020 20:13:21: 22000000 INFO @ Sun, 21 Jun 2020 20:13:25: 25000000 INFO @ Sun, 21 Jun 2020 20:13:27: 23000000 INFO @ Sun, 21 Jun 2020 20:13:32: 26000000 INFO @ Sun, 21 Jun 2020 20:13:34: 24000000 INFO @ Sun, 21 Jun 2020 20:13:39: 27000000 INFO @ Sun, 21 Jun 2020 20:13:40: 25000000 INFO @ Sun, 21 Jun 2020 20:13:46: 28000000 INFO @ Sun, 21 Jun 2020 20:13:47: 26000000 INFO @ Sun, 21 Jun 2020 20:13:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:13:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:13:49: #1 total tags in treatment: 28313123 INFO @ Sun, 21 Jun 2020 20:13:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:13:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:13:49: #1 tags after filtering in treatment: 28313122 INFO @ Sun, 21 Jun 2020 20:13:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:13:49: #1 finished! INFO @ Sun, 21 Jun 2020 20:13:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:13:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:13:51: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:13:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:13:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:13:53: 27000000 INFO @ Sun, 21 Jun 2020 20:13:58: 28000000 INFO @ Sun, 21 Jun 2020 20:14:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:14:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:14:00: #1 total tags in treatment: 28313123 INFO @ Sun, 21 Jun 2020 20:14:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:14:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:14:01: #1 tags after filtering in treatment: 28313122 INFO @ Sun, 21 Jun 2020 20:14:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:14:01: #1 finished! INFO @ Sun, 21 Jun 2020 20:14:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:14:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:14:03: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:14:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:14:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335489/SRX335489.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。