Job ID = 6456282 SRX = SRX335488 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:45:08 prefetch.2.10.7: 1) Downloading 'SRR952830'... 2020-06-21T10:45:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:49:49 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:49:49 prefetch.2.10.7: 1) 'SRR952830' was downloaded successfully Read 30649765 spots for SRR952830/SRR952830.sra Written 30649765 spots for SRR952830/SRR952830.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:40 30649765 reads; of these: 30649765 (100.00%) were unpaired; of these: 4101409 (13.38%) aligned 0 times 21534368 (70.26%) aligned exactly 1 time 5013988 (16.36%) aligned >1 times 86.62% overall alignment rate Time searching: 00:06:40 Overall time: 00:06:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7509772 / 26548356 = 0.2829 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:28: 1000000 INFO @ Sun, 21 Jun 2020 20:03:33: 2000000 INFO @ Sun, 21 Jun 2020 20:03:38: 3000000 INFO @ Sun, 21 Jun 2020 20:03:42: 4000000 INFO @ Sun, 21 Jun 2020 20:03:47: 5000000 INFO @ Sun, 21 Jun 2020 20:03:52: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:56: 7000000 INFO @ Sun, 21 Jun 2020 20:03:59: 1000000 INFO @ Sun, 21 Jun 2020 20:04:01: 8000000 INFO @ Sun, 21 Jun 2020 20:04:03: 2000000 INFO @ Sun, 21 Jun 2020 20:04:06: 9000000 INFO @ Sun, 21 Jun 2020 20:04:08: 3000000 INFO @ Sun, 21 Jun 2020 20:04:10: 10000000 INFO @ Sun, 21 Jun 2020 20:04:13: 4000000 INFO @ Sun, 21 Jun 2020 20:04:15: 11000000 INFO @ Sun, 21 Jun 2020 20:04:18: 5000000 INFO @ Sun, 21 Jun 2020 20:04:20: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:22: 6000000 INFO @ Sun, 21 Jun 2020 20:04:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:25: 13000000 INFO @ Sun, 21 Jun 2020 20:04:27: 7000000 INFO @ Sun, 21 Jun 2020 20:04:29: 1000000 INFO @ Sun, 21 Jun 2020 20:04:30: 14000000 INFO @ Sun, 21 Jun 2020 20:04:32: 8000000 INFO @ Sun, 21 Jun 2020 20:04:33: 2000000 INFO @ Sun, 21 Jun 2020 20:04:34: 15000000 INFO @ Sun, 21 Jun 2020 20:04:37: 9000000 INFO @ Sun, 21 Jun 2020 20:04:38: 3000000 INFO @ Sun, 21 Jun 2020 20:04:40: 16000000 INFO @ Sun, 21 Jun 2020 20:04:41: 10000000 INFO @ Sun, 21 Jun 2020 20:04:43: 4000000 INFO @ Sun, 21 Jun 2020 20:04:44: 17000000 INFO @ Sun, 21 Jun 2020 20:04:46: 11000000 INFO @ Sun, 21 Jun 2020 20:04:48: 5000000 INFO @ Sun, 21 Jun 2020 20:04:49: 18000000 INFO @ Sun, 21 Jun 2020 20:04:51: 12000000 INFO @ Sun, 21 Jun 2020 20:04:53: 6000000 INFO @ Sun, 21 Jun 2020 20:04:54: 19000000 INFO @ Sun, 21 Jun 2020 20:04:55: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:04:55: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:04:55: #1 total tags in treatment: 19038584 INFO @ Sun, 21 Jun 2020 20:04:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:04:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:04:55: #1 tags after filtering in treatment: 19038496 INFO @ Sun, 21 Jun 2020 20:04:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:04:55: #1 finished! INFO @ Sun, 21 Jun 2020 20:04:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:04:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:04:56: 13000000 INFO @ Sun, 21 Jun 2020 20:04:56: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 20:04:56: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:04:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:04:57: 7000000 INFO @ Sun, 21 Jun 2020 20:05:01: 14000000 INFO @ Sun, 21 Jun 2020 20:05:02: 8000000 INFO @ Sun, 21 Jun 2020 20:05:06: 15000000 INFO @ Sun, 21 Jun 2020 20:05:07: 9000000 INFO @ Sun, 21 Jun 2020 20:05:11: 16000000 INFO @ Sun, 21 Jun 2020 20:05:12: 10000000 INFO @ Sun, 21 Jun 2020 20:05:16: 17000000 INFO @ Sun, 21 Jun 2020 20:05:16: 11000000 INFO @ Sun, 21 Jun 2020 20:05:21: 18000000 INFO @ Sun, 21 Jun 2020 20:05:21: 12000000 INFO @ Sun, 21 Jun 2020 20:05:26: 19000000 INFO @ Sun, 21 Jun 2020 20:05:26: 13000000 INFO @ Sun, 21 Jun 2020 20:05:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:05:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:05:26: #1 total tags in treatment: 19038584 INFO @ Sun, 21 Jun 2020 20:05:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:05:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:05:27: #1 tags after filtering in treatment: 19038496 INFO @ Sun, 21 Jun 2020 20:05:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:05:27: #1 finished! INFO @ Sun, 21 Jun 2020 20:05:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:05:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:05:28: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 20:05:28: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:05:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:05:31: 14000000 INFO @ Sun, 21 Jun 2020 20:05:36: 15000000 INFO @ Sun, 21 Jun 2020 20:05:41: 16000000 INFO @ Sun, 21 Jun 2020 20:05:46: 17000000 INFO @ Sun, 21 Jun 2020 20:05:50: 18000000 INFO @ Sun, 21 Jun 2020 20:05:55: 19000000 INFO @ Sun, 21 Jun 2020 20:05:55: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:05:55: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:05:55: #1 total tags in treatment: 19038584 INFO @ Sun, 21 Jun 2020 20:05:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:05:56: #1 tags after filtering in treatment: 19038496 INFO @ Sun, 21 Jun 2020 20:05:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:05:56: #1 finished! INFO @ Sun, 21 Jun 2020 20:05:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:05:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:05:57: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 20:05:57: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:05:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335488/SRX335488.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。