Job ID = 6456281 SRX = SRX335487 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:41:38 prefetch.2.10.7: 1) Downloading 'SRR952829'... 2020-06-21T10:41:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:52:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:52:52 prefetch.2.10.7: 1) 'SRR952829' was downloaded successfully Read 67994326 spots for SRR952829/SRR952829.sra Written 67994326 spots for SRR952829/SRR952829.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:32 67994326 reads; of these: 67994326 (100.00%) were unpaired; of these: 10053596 (14.79%) aligned 0 times 46802123 (68.83%) aligned exactly 1 time 11138607 (16.38%) aligned >1 times 85.21% overall alignment rate Time searching: 00:16:32 Overall time: 00:16:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 23287180 / 57940730 = 0.4019 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:24:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:24:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:24:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:24:32: 1000000 INFO @ Sun, 21 Jun 2020 20:24:37: 2000000 INFO @ Sun, 21 Jun 2020 20:24:41: 3000000 INFO @ Sun, 21 Jun 2020 20:24:46: 4000000 INFO @ Sun, 21 Jun 2020 20:24:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:24:56: 6000000 INFO @ Sun, 21 Jun 2020 20:24:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:24:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:24:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:25:01: 7000000 INFO @ Sun, 21 Jun 2020 20:25:03: 1000000 INFO @ Sun, 21 Jun 2020 20:25:06: 8000000 INFO @ Sun, 21 Jun 2020 20:25:09: 2000000 INFO @ Sun, 21 Jun 2020 20:25:11: 9000000 INFO @ Sun, 21 Jun 2020 20:25:15: 3000000 INFO @ Sun, 21 Jun 2020 20:25:16: 10000000 INFO @ Sun, 21 Jun 2020 20:25:21: 4000000 INFO @ Sun, 21 Jun 2020 20:25:22: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:25:27: 5000000 INFO @ Sun, 21 Jun 2020 20:25:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:25:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:25:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:25:27: 12000000 INFO @ Sun, 21 Jun 2020 20:25:32: 13000000 INFO @ Sun, 21 Jun 2020 20:25:32: 1000000 INFO @ Sun, 21 Jun 2020 20:25:33: 6000000 INFO @ Sun, 21 Jun 2020 20:25:37: 14000000 INFO @ Sun, 21 Jun 2020 20:25:37: 2000000 INFO @ Sun, 21 Jun 2020 20:25:38: 7000000 INFO @ Sun, 21 Jun 2020 20:25:42: 15000000 INFO @ Sun, 21 Jun 2020 20:25:43: 3000000 INFO @ Sun, 21 Jun 2020 20:25:44: 8000000 INFO @ Sun, 21 Jun 2020 20:25:48: 16000000 INFO @ Sun, 21 Jun 2020 20:25:48: 4000000 INFO @ Sun, 21 Jun 2020 20:25:50: 9000000 INFO @ Sun, 21 Jun 2020 20:25:53: 17000000 INFO @ Sun, 21 Jun 2020 20:25:53: 5000000 INFO @ Sun, 21 Jun 2020 20:25:56: 10000000 INFO @ Sun, 21 Jun 2020 20:25:58: 18000000 INFO @ Sun, 21 Jun 2020 20:25:59: 6000000 INFO @ Sun, 21 Jun 2020 20:26:01: 11000000 INFO @ Sun, 21 Jun 2020 20:26:04: 19000000 INFO @ Sun, 21 Jun 2020 20:26:04: 7000000 INFO @ Sun, 21 Jun 2020 20:26:07: 12000000 INFO @ Sun, 21 Jun 2020 20:26:09: 20000000 INFO @ Sun, 21 Jun 2020 20:26:09: 8000000 INFO @ Sun, 21 Jun 2020 20:26:13: 13000000 INFO @ Sun, 21 Jun 2020 20:26:14: 21000000 INFO @ Sun, 21 Jun 2020 20:26:15: 9000000 INFO @ Sun, 21 Jun 2020 20:26:18: 14000000 INFO @ Sun, 21 Jun 2020 20:26:20: 22000000 INFO @ Sun, 21 Jun 2020 20:26:20: 10000000 INFO @ Sun, 21 Jun 2020 20:26:24: 15000000 INFO @ Sun, 21 Jun 2020 20:26:25: 23000000 INFO @ Sun, 21 Jun 2020 20:26:25: 11000000 INFO @ Sun, 21 Jun 2020 20:26:30: 16000000 INFO @ Sun, 21 Jun 2020 20:26:31: 24000000 INFO @ Sun, 21 Jun 2020 20:26:31: 12000000 INFO @ Sun, 21 Jun 2020 20:26:36: 17000000 INFO @ Sun, 21 Jun 2020 20:26:36: 25000000 INFO @ Sun, 21 Jun 2020 20:26:36: 13000000 INFO @ Sun, 21 Jun 2020 20:26:41: 26000000 INFO @ Sun, 21 Jun 2020 20:26:42: 18000000 INFO @ Sun, 21 Jun 2020 20:26:42: 14000000 INFO @ Sun, 21 Jun 2020 20:26:47: 27000000 INFO @ Sun, 21 Jun 2020 20:26:47: 15000000 INFO @ Sun, 21 Jun 2020 20:26:47: 19000000 INFO @ Sun, 21 Jun 2020 20:26:52: 28000000 INFO @ Sun, 21 Jun 2020 20:26:52: 16000000 INFO @ Sun, 21 Jun 2020 20:26:53: 20000000 INFO @ Sun, 21 Jun 2020 20:26:58: 29000000 INFO @ Sun, 21 Jun 2020 20:26:58: 17000000 INFO @ Sun, 21 Jun 2020 20:26:59: 21000000 INFO @ Sun, 21 Jun 2020 20:27:03: 30000000 INFO @ Sun, 21 Jun 2020 20:27:03: 18000000 INFO @ Sun, 21 Jun 2020 20:27:05: 22000000 INFO @ Sun, 21 Jun 2020 20:27:09: 31000000 INFO @ Sun, 21 Jun 2020 20:27:09: 19000000 INFO @ Sun, 21 Jun 2020 20:27:11: 23000000 INFO @ Sun, 21 Jun 2020 20:27:14: 32000000 INFO @ Sun, 21 Jun 2020 20:27:14: 20000000 INFO @ Sun, 21 Jun 2020 20:27:17: 24000000 INFO @ Sun, 21 Jun 2020 20:27:19: 33000000 INFO @ Sun, 21 Jun 2020 20:27:20: 21000000 INFO @ Sun, 21 Jun 2020 20:27:23: 25000000 INFO @ Sun, 21 Jun 2020 20:27:25: 34000000 INFO @ Sun, 21 Jun 2020 20:27:25: 22000000 INFO @ Sun, 21 Jun 2020 20:27:28: 26000000 INFO @ Sun, 21 Jun 2020 20:27:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:27:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:27:29: #1 total tags in treatment: 34653550 INFO @ Sun, 21 Jun 2020 20:27:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:27:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:27:29: #1 tags after filtering in treatment: 34653515 INFO @ Sun, 21 Jun 2020 20:27:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:27:29: #1 finished! INFO @ Sun, 21 Jun 2020 20:27:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:27:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:27:30: 23000000 INFO @ Sun, 21 Jun 2020 20:27:32: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:27:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:27:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:27:34: 27000000 INFO @ Sun, 21 Jun 2020 20:27:36: 24000000 INFO @ Sun, 21 Jun 2020 20:27:40: 28000000 INFO @ Sun, 21 Jun 2020 20:27:41: 25000000 INFO @ Sun, 21 Jun 2020 20:27:46: 29000000 INFO @ Sun, 21 Jun 2020 20:27:46: 26000000 INFO @ Sun, 21 Jun 2020 20:27:52: 27000000 INFO @ Sun, 21 Jun 2020 20:27:52: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:27:58: 28000000 INFO @ Sun, 21 Jun 2020 20:27:58: 31000000 INFO @ Sun, 21 Jun 2020 20:28:03: 29000000 INFO @ Sun, 21 Jun 2020 20:28:04: 32000000 INFO @ Sun, 21 Jun 2020 20:28:08: 30000000 INFO @ Sun, 21 Jun 2020 20:28:10: 33000000 INFO @ Sun, 21 Jun 2020 20:28:13: 31000000 INFO @ Sun, 21 Jun 2020 20:28:16: 34000000 INFO @ Sun, 21 Jun 2020 20:28:19: 32000000 INFO @ Sun, 21 Jun 2020 20:28:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:28:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:28:20: #1 total tags in treatment: 34653550 INFO @ Sun, 21 Jun 2020 20:28:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:28:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:28:21: #1 tags after filtering in treatment: 34653515 INFO @ Sun, 21 Jun 2020 20:28:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:28:21: #1 finished! INFO @ Sun, 21 Jun 2020 20:28:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:28:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:28:23: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:28:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:28:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:28:24: 33000000 INFO @ Sun, 21 Jun 2020 20:28:29: 34000000 INFO @ Sun, 21 Jun 2020 20:28:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:28:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:28:33: #1 total tags in treatment: 34653550 INFO @ Sun, 21 Jun 2020 20:28:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:28:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:28:33: #1 tags after filtering in treatment: 34653515 INFO @ Sun, 21 Jun 2020 20:28:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:28:33: #1 finished! INFO @ Sun, 21 Jun 2020 20:28:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:28:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:28:35: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:28:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:28:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335487/SRX335487.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。