Job ID = 6456280 SRX = SRX335486 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:39:08 prefetch.2.10.7: 1) Downloading 'SRR952828'... 2020-06-21T10:39:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:47:15 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:47:15 prefetch.2.10.7: 1) 'SRR952828' was downloaded successfully Read 47935093 spots for SRR952828/SRR952828.sra Written 47935093 spots for SRR952828/SRR952828.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:11 47935093 reads; of these: 47935093 (100.00%) were unpaired; of these: 4078669 (8.51%) aligned 0 times 36554395 (76.26%) aligned exactly 1 time 7302029 (15.23%) aligned >1 times 91.49% overall alignment rate Time searching: 00:12:11 Overall time: 00:12:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 17039392 / 43856424 = 0.3885 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:11: 1000000 INFO @ Sun, 21 Jun 2020 20:11:17: 2000000 INFO @ Sun, 21 Jun 2020 20:11:23: 3000000 INFO @ Sun, 21 Jun 2020 20:11:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:37: 5000000 INFO @ Sun, 21 Jun 2020 20:11:41: 1000000 INFO @ Sun, 21 Jun 2020 20:11:44: 6000000 INFO @ Sun, 21 Jun 2020 20:11:48: 2000000 INFO @ Sun, 21 Jun 2020 20:11:51: 7000000 INFO @ Sun, 21 Jun 2020 20:11:55: 3000000 INFO @ Sun, 21 Jun 2020 20:11:58: 8000000 INFO @ Sun, 21 Jun 2020 20:12:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:12:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:12:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:12:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:05: 9000000 INFO @ Sun, 21 Jun 2020 20:12:09: 5000000 INFO @ Sun, 21 Jun 2020 20:12:11: 1000000 INFO @ Sun, 21 Jun 2020 20:12:12: 10000000 INFO @ Sun, 21 Jun 2020 20:12:16: 6000000 INFO @ Sun, 21 Jun 2020 20:12:19: 2000000 INFO @ Sun, 21 Jun 2020 20:12:20: 11000000 INFO @ Sun, 21 Jun 2020 20:12:23: 7000000 INFO @ Sun, 21 Jun 2020 20:12:26: 3000000 INFO @ Sun, 21 Jun 2020 20:12:27: 12000000 INFO @ Sun, 21 Jun 2020 20:12:30: 8000000 INFO @ Sun, 21 Jun 2020 20:12:33: 4000000 INFO @ Sun, 21 Jun 2020 20:12:34: 13000000 INFO @ Sun, 21 Jun 2020 20:12:37: 9000000 INFO @ Sun, 21 Jun 2020 20:12:40: 5000000 INFO @ Sun, 21 Jun 2020 20:12:41: 14000000 INFO @ Sun, 21 Jun 2020 20:12:44: 10000000 INFO @ Sun, 21 Jun 2020 20:12:47: 6000000 INFO @ Sun, 21 Jun 2020 20:12:48: 15000000 INFO @ Sun, 21 Jun 2020 20:12:51: 11000000 INFO @ Sun, 21 Jun 2020 20:12:54: 7000000 INFO @ Sun, 21 Jun 2020 20:12:55: 16000000 INFO @ Sun, 21 Jun 2020 20:12:58: 12000000 INFO @ Sun, 21 Jun 2020 20:13:02: 8000000 INFO @ Sun, 21 Jun 2020 20:13:02: 17000000 INFO @ Sun, 21 Jun 2020 20:13:05: 13000000 INFO @ Sun, 21 Jun 2020 20:13:09: 9000000 INFO @ Sun, 21 Jun 2020 20:13:09: 18000000 INFO @ Sun, 21 Jun 2020 20:13:12: 14000000 INFO @ Sun, 21 Jun 2020 20:13:16: 10000000 INFO @ Sun, 21 Jun 2020 20:13:16: 19000000 INFO @ Sun, 21 Jun 2020 20:13:19: 15000000 INFO @ Sun, 21 Jun 2020 20:13:23: 11000000 INFO @ Sun, 21 Jun 2020 20:13:23: 20000000 INFO @ Sun, 21 Jun 2020 20:13:26: 16000000 INFO @ Sun, 21 Jun 2020 20:13:30: 12000000 INFO @ Sun, 21 Jun 2020 20:13:30: 21000000 INFO @ Sun, 21 Jun 2020 20:13:33: 17000000 INFO @ Sun, 21 Jun 2020 20:13:37: 13000000 INFO @ Sun, 21 Jun 2020 20:13:38: 22000000 INFO @ Sun, 21 Jun 2020 20:13:40: 18000000 INFO @ Sun, 21 Jun 2020 20:13:44: 14000000 INFO @ Sun, 21 Jun 2020 20:13:44: 23000000 INFO @ Sun, 21 Jun 2020 20:13:47: 19000000 INFO @ Sun, 21 Jun 2020 20:13:51: 15000000 INFO @ Sun, 21 Jun 2020 20:13:52: 24000000 INFO @ Sun, 21 Jun 2020 20:13:54: 20000000 INFO @ Sun, 21 Jun 2020 20:13:58: 16000000 INFO @ Sun, 21 Jun 2020 20:13:59: 25000000 INFO @ Sun, 21 Jun 2020 20:14:01: 21000000 INFO @ Sun, 21 Jun 2020 20:14:06: 26000000 INFO @ Sun, 21 Jun 2020 20:14:06: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:14:08: 22000000 INFO @ Sun, 21 Jun 2020 20:14:12: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:14:12: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:14:12: #1 total tags in treatment: 26817032 INFO @ Sun, 21 Jun 2020 20:14:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:14:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:14:12: #1 tags after filtering in treatment: 26816979 INFO @ Sun, 21 Jun 2020 20:14:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:14:12: #1 finished! INFO @ Sun, 21 Jun 2020 20:14:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:14:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:14:13: 18000000 INFO @ Sun, 21 Jun 2020 20:14:14: #2 number of paired peaks: 8 WARNING @ Sun, 21 Jun 2020 20:14:14: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:14:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:14:15: 23000000 INFO @ Sun, 21 Jun 2020 20:14:20: 19000000 INFO @ Sun, 21 Jun 2020 20:14:23: 24000000 INFO @ Sun, 21 Jun 2020 20:14:27: 20000000 INFO @ Sun, 21 Jun 2020 20:14:30: 25000000 INFO @ Sun, 21 Jun 2020 20:14:34: 21000000 INFO @ Sun, 21 Jun 2020 20:14:37: 26000000 INFO @ Sun, 21 Jun 2020 20:14:42: 22000000 INFO @ Sun, 21 Jun 2020 20:14:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:14:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:14:43: #1 total tags in treatment: 26817032 INFO @ Sun, 21 Jun 2020 20:14:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:14:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:14:44: #1 tags after filtering in treatment: 26816979 INFO @ Sun, 21 Jun 2020 20:14:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:14:44: #1 finished! INFO @ Sun, 21 Jun 2020 20:14:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:14:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:14:45: #2 number of paired peaks: 8 WARNING @ Sun, 21 Jun 2020 20:14:45: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:14:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:14:48: 23000000 INFO @ Sun, 21 Jun 2020 20:14:55: 24000000 INFO @ Sun, 21 Jun 2020 20:15:01: 25000000 INFO @ Sun, 21 Jun 2020 20:15:08: 26000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:15:13: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:15:13: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:15:13: #1 total tags in treatment: 26817032 INFO @ Sun, 21 Jun 2020 20:15:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:15:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:15:14: #1 tags after filtering in treatment: 26816979 INFO @ Sun, 21 Jun 2020 20:15:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:15:14: #1 finished! INFO @ Sun, 21 Jun 2020 20:15:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:15:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:15:16: #2 number of paired peaks: 8 WARNING @ Sun, 21 Jun 2020 20:15:16: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:15:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX335486/SRX335486.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling