Job ID = 6456262 SRX = SRX331412 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:39:38 prefetch.2.10.7: 1) Downloading 'SRR947649'... 2020-06-21T10:39:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:41:20 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:41:20 prefetch.2.10.7: 'SRR947649' is valid 2020-06-21T10:41:20 prefetch.2.10.7: 1) 'SRR947649' was downloaded successfully Read 11235844 spots for SRR947649/SRR947649.sra Written 11235844 spots for SRR947649/SRR947649.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:02:38 11235844 reads; of these: 11235844 (100.00%) were unpaired; of these: 662663 (5.90%) aligned 0 times 9033788 (80.40%) aligned exactly 1 time 1539393 (13.70%) aligned >1 times 94.10% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 838850 / 10573181 = 0.0793 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:47:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:47:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:47:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:47:48: 1000000 INFO @ Sun, 21 Jun 2020 19:47:55: 2000000 INFO @ Sun, 21 Jun 2020 19:48:02: 3000000 INFO @ Sun, 21 Jun 2020 19:48:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:48:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:48:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:48:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:48:15: 5000000 INFO @ Sun, 21 Jun 2020 19:48:18: 1000000 INFO @ Sun, 21 Jun 2020 19:48:23: 6000000 INFO @ Sun, 21 Jun 2020 19:48:24: 2000000 INFO @ Sun, 21 Jun 2020 19:48:30: 7000000 INFO @ Sun, 21 Jun 2020 19:48:31: 3000000 INFO @ Sun, 21 Jun 2020 19:48:38: 8000000 INFO @ Sun, 21 Jun 2020 19:48:38: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:48:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:48:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:48:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:48:45: 5000000 INFO @ Sun, 21 Jun 2020 19:48:45: 9000000 INFO @ Sun, 21 Jun 2020 19:48:48: 1000000 INFO @ Sun, 21 Jun 2020 19:48:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:48:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:48:51: #1 total tags in treatment: 9734331 INFO @ Sun, 21 Jun 2020 19:48:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:48:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:48:51: #1 tags after filtering in treatment: 9734310 INFO @ Sun, 21 Jun 2020 19:48:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:48:51: #1 finished! INFO @ Sun, 21 Jun 2020 19:48:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:48:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:48:52: 6000000 INFO @ Sun, 21 Jun 2020 19:48:52: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 19:48:52: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:48:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:48:55: 2000000 INFO @ Sun, 21 Jun 2020 19:48:58: 7000000 INFO @ Sun, 21 Jun 2020 19:49:01: 3000000 INFO @ Sun, 21 Jun 2020 19:49:04: 8000000 INFO @ Sun, 21 Jun 2020 19:49:07: 4000000 INFO @ Sun, 21 Jun 2020 19:49:10: 9000000 INFO @ Sun, 21 Jun 2020 19:49:13: 5000000 INFO @ Sun, 21 Jun 2020 19:49:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:49:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:49:15: #1 total tags in treatment: 9734331 INFO @ Sun, 21 Jun 2020 19:49:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:49:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:49:16: #1 tags after filtering in treatment: 9734310 INFO @ Sun, 21 Jun 2020 19:49:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:49:16: #1 finished! INFO @ Sun, 21 Jun 2020 19:49:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:49:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:49:16: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 19:49:16: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:49:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:49:19: 6000000 INFO @ Sun, 21 Jun 2020 19:49:25: 7000000 INFO @ Sun, 21 Jun 2020 19:49:30: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:49:36: 9000000 INFO @ Sun, 21 Jun 2020 19:49:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:49:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:49:41: #1 total tags in treatment: 9734331 INFO @ Sun, 21 Jun 2020 19:49:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:49:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:49:41: #1 tags after filtering in treatment: 9734310 INFO @ Sun, 21 Jun 2020 19:49:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:49:41: #1 finished! INFO @ Sun, 21 Jun 2020 19:49:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:49:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:49:42: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 19:49:42: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:49:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331412/SRX331412.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。