Job ID = 6456259 SRX = SRX331411 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:50:46 prefetch.2.10.7: 1) Downloading 'SRR947648'... 2020-06-21T10:50:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:53:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:53:45 prefetch.2.10.7: 'SRR947648' is valid 2020-06-21T10:53:45 prefetch.2.10.7: 1) 'SRR947648' was downloaded successfully Read 11907599 spots for SRR947648/SRR947648.sra Written 11907599 spots for SRR947648/SRR947648.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:47 11907599 reads; of these: 11907599 (100.00%) were unpaired; of these: 585282 (4.92%) aligned 0 times 9692997 (81.40%) aligned exactly 1 time 1629320 (13.68%) aligned >1 times 95.08% overall alignment rate Time searching: 00:02:47 Overall time: 00:02:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 936036 / 11322317 = 0.0827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:01:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:01:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:01:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:01:25: 1000000 INFO @ Sun, 21 Jun 2020 20:01:31: 2000000 INFO @ Sun, 21 Jun 2020 20:01:38: 3000000 INFO @ Sun, 21 Jun 2020 20:01:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:01:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:01:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:01:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:01:52: 5000000 INFO @ Sun, 21 Jun 2020 20:01:56: 1000000 INFO @ Sun, 21 Jun 2020 20:02:00: 6000000 INFO @ Sun, 21 Jun 2020 20:02:04: 2000000 INFO @ Sun, 21 Jun 2020 20:02:08: 7000000 INFO @ Sun, 21 Jun 2020 20:02:12: 3000000 INFO @ Sun, 21 Jun 2020 20:02:15: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:02:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:02:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:02:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:02:20: 4000000 INFO @ Sun, 21 Jun 2020 20:02:24: 9000000 INFO @ Sun, 21 Jun 2020 20:02:27: 1000000 INFO @ Sun, 21 Jun 2020 20:02:28: 5000000 INFO @ Sun, 21 Jun 2020 20:02:32: 10000000 INFO @ Sun, 21 Jun 2020 20:02:35: 2000000 INFO @ Sun, 21 Jun 2020 20:02:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:02:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:02:36: #1 total tags in treatment: 10386281 INFO @ Sun, 21 Jun 2020 20:02:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:02:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:02:36: #1 tags after filtering in treatment: 10386258 INFO @ Sun, 21 Jun 2020 20:02:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:02:36: #1 finished! INFO @ Sun, 21 Jun 2020 20:02:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:02:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:02:37: 6000000 INFO @ Sun, 21 Jun 2020 20:02:37: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 20:02:37: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:02:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:02:43: 3000000 INFO @ Sun, 21 Jun 2020 20:02:44: 7000000 INFO @ Sun, 21 Jun 2020 20:02:51: 4000000 INFO @ Sun, 21 Jun 2020 20:02:52: 8000000 INFO @ Sun, 21 Jun 2020 20:02:58: 5000000 INFO @ Sun, 21 Jun 2020 20:03:00: 9000000 INFO @ Sun, 21 Jun 2020 20:03:06: 6000000 INFO @ Sun, 21 Jun 2020 20:03:08: 10000000 INFO @ Sun, 21 Jun 2020 20:03:11: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:03:11: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:03:11: #1 total tags in treatment: 10386281 INFO @ Sun, 21 Jun 2020 20:03:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:03:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:03:12: #1 tags after filtering in treatment: 10386258 INFO @ Sun, 21 Jun 2020 20:03:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:03:12: #1 finished! INFO @ Sun, 21 Jun 2020 20:03:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:03:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:03:12: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 20:03:12: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:03:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:03:13: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:03:20: 8000000 INFO @ Sun, 21 Jun 2020 20:03:27: 9000000 INFO @ Sun, 21 Jun 2020 20:03:34: 10000000 INFO @ Sun, 21 Jun 2020 20:03:37: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:03:37: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:03:37: #1 total tags in treatment: 10386281 INFO @ Sun, 21 Jun 2020 20:03:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:03:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:03:38: #1 tags after filtering in treatment: 10386258 INFO @ Sun, 21 Jun 2020 20:03:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:03:38: #1 finished! INFO @ Sun, 21 Jun 2020 20:03:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:03:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:03:38: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 20:03:38: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:03:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331411/SRX331411.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。