Job ID = 6456233 SRX = SRX331387 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:46:23 prefetch.2.10.7: 1) Downloading 'SRR947624'... 2020-06-21T10:46:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:49:21 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:49:22 prefetch.2.10.7: 'SRR947624' is valid 2020-06-21T10:49:22 prefetch.2.10.7: 1) 'SRR947624' was downloaded successfully Read 14046861 spots for SRR947624/SRR947624.sra Written 14046861 spots for SRR947624/SRR947624.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:58 14046861 reads; of these: 14046861 (100.00%) were unpaired; of these: 930608 (6.63%) aligned 0 times 12370838 (88.07%) aligned exactly 1 time 745415 (5.31%) aligned >1 times 93.37% overall alignment rate Time searching: 00:02:58 Overall time: 00:02:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1223957 / 13116253 = 0.0933 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:56:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:56:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:56:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:57:05: 1000000 INFO @ Sun, 21 Jun 2020 19:57:11: 2000000 INFO @ Sun, 21 Jun 2020 19:57:16: 3000000 INFO @ Sun, 21 Jun 2020 19:57:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:57:28: 5000000 INFO @ Sun, 21 Jun 2020 19:57:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:57:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:57:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:57:33: 6000000 INFO @ Sun, 21 Jun 2020 19:57:34: 1000000 INFO @ Sun, 21 Jun 2020 19:57:39: 7000000 INFO @ Sun, 21 Jun 2020 19:57:40: 2000000 INFO @ Sun, 21 Jun 2020 19:57:45: 8000000 INFO @ Sun, 21 Jun 2020 19:57:45: 3000000 INFO @ Sun, 21 Jun 2020 19:57:50: 9000000 INFO @ Sun, 21 Jun 2020 19:57:51: 4000000 INFO @ Sun, 21 Jun 2020 19:57:55: 10000000 INFO @ Sun, 21 Jun 2020 19:57:56: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:57:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:57:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:57:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:58:01: 11000000 INFO @ Sun, 21 Jun 2020 19:58:02: 6000000 INFO @ Sun, 21 Jun 2020 19:58:04: 1000000 INFO @ Sun, 21 Jun 2020 19:58:06: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:58:06: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:58:06: #1 total tags in treatment: 11892296 INFO @ Sun, 21 Jun 2020 19:58:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:58:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:58:07: #1 tags after filtering in treatment: 11892030 INFO @ Sun, 21 Jun 2020 19:58:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:58:07: #1 finished! INFO @ Sun, 21 Jun 2020 19:58:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:58:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:58:07: 7000000 INFO @ Sun, 21 Jun 2020 19:58:07: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:58:07: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:58:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:58:10: 2000000 INFO @ Sun, 21 Jun 2020 19:58:13: 8000000 INFO @ Sun, 21 Jun 2020 19:58:15: 3000000 INFO @ Sun, 21 Jun 2020 19:58:18: 9000000 INFO @ Sun, 21 Jun 2020 19:58:20: 4000000 INFO @ Sun, 21 Jun 2020 19:58:23: 10000000 INFO @ Sun, 21 Jun 2020 19:58:26: 5000000 INFO @ Sun, 21 Jun 2020 19:58:29: 11000000 INFO @ Sun, 21 Jun 2020 19:58:31: 6000000 INFO @ Sun, 21 Jun 2020 19:58:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:58:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:58:34: #1 total tags in treatment: 11892296 INFO @ Sun, 21 Jun 2020 19:58:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:58:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:58:34: #1 tags after filtering in treatment: 11892030 INFO @ Sun, 21 Jun 2020 19:58:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:58:34: #1 finished! INFO @ Sun, 21 Jun 2020 19:58:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:58:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:58:35: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:58:35: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:58:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:58:36: 7000000 INFO @ Sun, 21 Jun 2020 19:58:42: 8000000 INFO @ Sun, 21 Jun 2020 19:58:47: 9000000 INFO @ Sun, 21 Jun 2020 19:58:52: 10000000 INFO @ Sun, 21 Jun 2020 19:58:57: 11000000 INFO @ Sun, 21 Jun 2020 19:59:02: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:59:02: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:59:02: #1 total tags in treatment: 11892296 INFO @ Sun, 21 Jun 2020 19:59:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:59:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:59:03: #1 tags after filtering in treatment: 11892030 INFO @ Sun, 21 Jun 2020 19:59:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:59:03: #1 finished! INFO @ Sun, 21 Jun 2020 19:59:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:59:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:59:03: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:59:03: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:59:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX331387/SRX331387.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。