Job ID = 6529583 SRX = SRX330269 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:16 76489645 reads; of these: 76489645 (100.00%) were unpaired; of these: 5335955 (6.98%) aligned 0 times 47918464 (62.65%) aligned exactly 1 time 23235226 (30.38%) aligned >1 times 93.02% overall alignment rate Time searching: 00:22:16 Overall time: 00:22:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 37392245 / 71153690 = 0.5255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:05:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:05:58: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:05:58: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:06:06: 1000000 INFO @ Tue, 30 Jun 2020 03:06:14: 2000000 INFO @ Tue, 30 Jun 2020 03:06:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:06:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:06:28: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:06:28: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:06:29: 4000000 INFO @ Tue, 30 Jun 2020 03:06:36: 1000000 INFO @ Tue, 30 Jun 2020 03:06:36: 5000000 INFO @ Tue, 30 Jun 2020 03:06:43: 6000000 INFO @ Tue, 30 Jun 2020 03:06:43: 2000000 INFO @ Tue, 30 Jun 2020 03:06:51: 7000000 INFO @ Tue, 30 Jun 2020 03:06:51: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:06:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:06:58: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:06:58: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:06:58: 8000000 INFO @ Tue, 30 Jun 2020 03:06:58: 4000000 INFO @ Tue, 30 Jun 2020 03:07:06: 1000000 INFO @ Tue, 30 Jun 2020 03:07:06: 9000000 INFO @ Tue, 30 Jun 2020 03:07:06: 5000000 INFO @ Tue, 30 Jun 2020 03:07:13: 10000000 INFO @ Tue, 30 Jun 2020 03:07:13: 6000000 INFO @ Tue, 30 Jun 2020 03:07:13: 2000000 INFO @ Tue, 30 Jun 2020 03:07:20: 3000000 INFO @ Tue, 30 Jun 2020 03:07:20: 11000000 INFO @ Tue, 30 Jun 2020 03:07:20: 7000000 INFO @ Tue, 30 Jun 2020 03:07:26: 4000000 INFO @ Tue, 30 Jun 2020 03:07:28: 8000000 INFO @ Tue, 30 Jun 2020 03:07:28: 12000000 INFO @ Tue, 30 Jun 2020 03:07:32: 5000000 INFO @ Tue, 30 Jun 2020 03:07:35: 9000000 INFO @ Tue, 30 Jun 2020 03:07:35: 13000000 INFO @ Tue, 30 Jun 2020 03:07:38: 6000000 INFO @ Tue, 30 Jun 2020 03:07:43: 10000000 INFO @ Tue, 30 Jun 2020 03:07:43: 14000000 INFO @ Tue, 30 Jun 2020 03:07:44: 7000000 INFO @ Tue, 30 Jun 2020 03:07:50: 11000000 INFO @ Tue, 30 Jun 2020 03:07:51: 8000000 INFO @ Tue, 30 Jun 2020 03:07:51: 15000000 INFO @ Tue, 30 Jun 2020 03:07:57: 9000000 INFO @ Tue, 30 Jun 2020 03:07:58: 12000000 INFO @ Tue, 30 Jun 2020 03:07:59: 16000000 INFO @ Tue, 30 Jun 2020 03:08:03: 10000000 INFO @ Tue, 30 Jun 2020 03:08:06: 13000000 INFO @ Tue, 30 Jun 2020 03:08:07: 17000000 INFO @ Tue, 30 Jun 2020 03:08:10: 11000000 INFO @ Tue, 30 Jun 2020 03:08:14: 14000000 INFO @ Tue, 30 Jun 2020 03:08:14: 18000000 INFO @ Tue, 30 Jun 2020 03:08:16: 12000000 INFO @ Tue, 30 Jun 2020 03:08:21: 15000000 INFO @ Tue, 30 Jun 2020 03:08:22: 19000000 INFO @ Tue, 30 Jun 2020 03:08:22: 13000000 INFO @ Tue, 30 Jun 2020 03:08:28: 16000000 INFO @ Tue, 30 Jun 2020 03:08:29: 14000000 INFO @ Tue, 30 Jun 2020 03:08:29: 20000000 INFO @ Tue, 30 Jun 2020 03:08:35: 15000000 INFO @ Tue, 30 Jun 2020 03:08:35: 17000000 INFO @ Tue, 30 Jun 2020 03:08:35: 21000000 INFO @ Tue, 30 Jun 2020 03:08:41: 16000000 INFO @ Tue, 30 Jun 2020 03:08:41: 18000000 INFO @ Tue, 30 Jun 2020 03:08:42: 22000000 INFO @ Tue, 30 Jun 2020 03:08:47: 17000000 INFO @ Tue, 30 Jun 2020 03:08:48: 19000000 INFO @ Tue, 30 Jun 2020 03:08:48: 23000000 INFO @ Tue, 30 Jun 2020 03:08:53: 18000000 INFO @ Tue, 30 Jun 2020 03:08:55: 20000000 INFO @ Tue, 30 Jun 2020 03:08:55: 24000000 INFO @ Tue, 30 Jun 2020 03:08:59: 19000000 INFO @ Tue, 30 Jun 2020 03:09:02: 21000000 INFO @ Tue, 30 Jun 2020 03:09:02: 25000000 INFO @ Tue, 30 Jun 2020 03:09:05: 20000000 INFO @ Tue, 30 Jun 2020 03:09:08: 22000000 INFO @ Tue, 30 Jun 2020 03:09:09: 26000000 INFO @ Tue, 30 Jun 2020 03:09:11: 21000000 INFO @ Tue, 30 Jun 2020 03:09:15: 23000000 INFO @ Tue, 30 Jun 2020 03:09:15: 27000000 INFO @ Tue, 30 Jun 2020 03:09:17: 22000000 INFO @ Tue, 30 Jun 2020 03:09:22: 24000000 INFO @ Tue, 30 Jun 2020 03:09:22: 23000000 INFO @ Tue, 30 Jun 2020 03:09:22: 28000000 INFO @ Tue, 30 Jun 2020 03:09:28: 24000000 INFO @ Tue, 30 Jun 2020 03:09:28: 25000000 INFO @ Tue, 30 Jun 2020 03:09:29: 29000000 INFO @ Tue, 30 Jun 2020 03:09:34: 25000000 INFO @ Tue, 30 Jun 2020 03:09:35: 26000000 INFO @ Tue, 30 Jun 2020 03:09:36: 30000000 INFO @ Tue, 30 Jun 2020 03:09:40: 26000000 INFO @ Tue, 30 Jun 2020 03:09:41: 27000000 INFO @ Tue, 30 Jun 2020 03:09:42: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 03:09:45: 27000000 INFO @ Tue, 30 Jun 2020 03:09:48: 28000000 INFO @ Tue, 30 Jun 2020 03:09:48: 32000000 INFO @ Tue, 30 Jun 2020 03:09:51: 28000000 INFO @ Tue, 30 Jun 2020 03:09:54: 33000000 INFO @ Tue, 30 Jun 2020 03:09:54: 29000000 INFO @ Tue, 30 Jun 2020 03:09:57: 29000000 INFO @ Tue, 30 Jun 2020 03:09:59: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:09:59: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:09:59: #1 total tags in treatment: 33761445 INFO @ Tue, 30 Jun 2020 03:09:59: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:09:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:10:00: #1 tags after filtering in treatment: 33761402 INFO @ Tue, 30 Jun 2020 03:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:10:00: #1 finished! INFO @ Tue, 30 Jun 2020 03:10:00: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:10:00: 30000000 INFO @ Tue, 30 Jun 2020 03:10:02: #2 number of paired peaks: 1144 INFO @ Tue, 30 Jun 2020 03:10:02: start model_add_line... INFO @ Tue, 30 Jun 2020 03:10:03: start X-correlation... INFO @ Tue, 30 Jun 2020 03:10:03: end of X-cor INFO @ Tue, 30 Jun 2020 03:10:03: #2 finished! INFO @ Tue, 30 Jun 2020 03:10:03: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 03:10:03: #2 alternative fragment length(s) may be 0,22,45,571 bps INFO @ Tue, 30 Jun 2020 03:10:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.05_model.r WARNING @ Tue, 30 Jun 2020 03:10:03: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:10:03: #2 You may need to consider one of the other alternative d(s): 0,22,45,571 WARNING @ Tue, 30 Jun 2020 03:10:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:10:03: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:10:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:10:03: 30000000 INFO @ Tue, 30 Jun 2020 03:10:06: 31000000 INFO @ Tue, 30 Jun 2020 03:10:09: 31000000 INFO @ Tue, 30 Jun 2020 03:10:11: 32000000 INFO @ Tue, 30 Jun 2020 03:10:14: 32000000 INFO @ Tue, 30 Jun 2020 03:10:17: 33000000 INFO @ Tue, 30 Jun 2020 03:10:20: 33000000 INFO @ Tue, 30 Jun 2020 03:10:21: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:10:21: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:10:21: #1 total tags in treatment: 33761445 INFO @ Tue, 30 Jun 2020 03:10:21: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:10:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:10:22: #1 tags after filtering in treatment: 33761402 INFO @ Tue, 30 Jun 2020 03:10:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:10:22: #1 finished! INFO @ Tue, 30 Jun 2020 03:10:22: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:10:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:10:25: #2 number of paired peaks: 1144 INFO @ Tue, 30 Jun 2020 03:10:25: start model_add_line... INFO @ Tue, 30 Jun 2020 03:10:25: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:10:25: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:10:25: #1 total tags in treatment: 33761445 INFO @ Tue, 30 Jun 2020 03:10:25: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:10:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:10:25: start X-correlation... INFO @ Tue, 30 Jun 2020 03:10:25: end of X-cor INFO @ Tue, 30 Jun 2020 03:10:25: #2 finished! INFO @ Tue, 30 Jun 2020 03:10:25: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 03:10:25: #2 alternative fragment length(s) may be 0,22,45,571 bps INFO @ Tue, 30 Jun 2020 03:10:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.10_model.r WARNING @ Tue, 30 Jun 2020 03:10:25: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:10:25: #2 You may need to consider one of the other alternative d(s): 0,22,45,571 WARNING @ Tue, 30 Jun 2020 03:10:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:10:25: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:10:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:10:26: #1 tags after filtering in treatment: 33761402 INFO @ Tue, 30 Jun 2020 03:10:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:10:26: #1 finished! INFO @ Tue, 30 Jun 2020 03:10:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:10:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:10:28: #2 number of paired peaks: 1144 INFO @ Tue, 30 Jun 2020 03:10:28: start model_add_line... INFO @ Tue, 30 Jun 2020 03:10:28: start X-correlation... INFO @ Tue, 30 Jun 2020 03:10:28: end of X-cor INFO @ Tue, 30 Jun 2020 03:10:28: #2 finished! INFO @ Tue, 30 Jun 2020 03:10:28: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 03:10:28: #2 alternative fragment length(s) may be 0,22,45,571 bps INFO @ Tue, 30 Jun 2020 03:10:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX330269/SRX330269.20_model.r WARNING @ Tue, 30 Jun 2020 03:10:28: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:10:28: #2 You may need to consider one of the other alternative d(s): 0,22,45,571 WARNING @ Tue, 30 Jun 2020 03:10:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:10:28: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:10:28: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 /var/spool/uge/it006/job_scripts/6529583: line 293: 23973 Terminated MACS $i /var/spool/uge/it006/job_scripts/6529583: line 293: 24237 Terminated MACS $i /var/spool/uge/it006/job_scripts/6529583: line 293: 24327 Terminated MACS $i ls: cannot access SRX330269.05.bed: No such file or directory mv: cannot stat ‘SRX330269.05.bed’: No such file or directory mv: cannot stat ‘SRX330269.05.bb’: No such file or directory ls: cannot access SRX330269.10.bed: No such file or directory mv: cannot stat ‘SRX330269.10.bed’: No such file or directory mv: cannot stat ‘SRX330269.10.bb’: No such file or directory ls: cannot access SRX330269.20.bed: No such file or directory mv: cannot stat ‘SRX330269.20.bed’: No such file or directory mv: cannot stat ‘SRX330269.20.bb’: No such file or directory