Job ID = 14172762 SRX = SRX3293005 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:05:42 13458267 reads; of these: 13458267 (100.00%) were unpaired; of these: 500809 (3.72%) aligned 0 times 12084299 (89.79%) aligned exactly 1 time 873159 (6.49%) aligned >1 times 96.28% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4741182 / 12957458 = 0.3659 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:02:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:02:59: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:02:59: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:03:13: 1000000 INFO @ Sat, 11 Dec 2021 17:03:26: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:03:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:03:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:03:41: 3000000 INFO @ Sat, 11 Dec 2021 17:03:46: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 17:03:58: 4000000 INFO @ Sat, 11 Dec 2021 17:03:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 17:03:59: #1 read tag files... INFO @ Sat, 11 Dec 2021 17:03:59: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 17:04:02: 2000000 INFO @ Sat, 11 Dec 2021 17:04:13: 1000000 INFO @ Sat, 11 Dec 2021 17:04:15: 5000000 INFO @ Sat, 11 Dec 2021 17:04:19: 3000000 INFO @ Sat, 11 Dec 2021 17:04:29: 2000000 INFO @ Sat, 11 Dec 2021 17:04:31: 6000000 INFO @ Sat, 11 Dec 2021 17:04:35: 4000000 INFO @ Sat, 11 Dec 2021 17:04:45: 3000000 INFO @ Sat, 11 Dec 2021 17:04:48: 7000000 INFO @ Sat, 11 Dec 2021 17:04:51: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 17:05:00: 4000000 INFO @ Sat, 11 Dec 2021 17:05:05: 8000000 INFO @ Sat, 11 Dec 2021 17:05:08: 6000000 INFO @ Sat, 11 Dec 2021 17:05:09: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 17:05:09: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 17:05:09: #1 total tags in treatment: 8216276 INFO @ Sat, 11 Dec 2021 17:05:09: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:05:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:05:09: #1 tags after filtering in treatment: 8215864 INFO @ Sat, 11 Dec 2021 17:05:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:05:09: #1 finished! INFO @ Sat, 11 Dec 2021 17:05:09: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:05:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:05:11: #2 number of paired peaks: 9331 INFO @ Sat, 11 Dec 2021 17:05:11: start model_add_line... INFO @ Sat, 11 Dec 2021 17:05:11: start X-correlation... INFO @ Sat, 11 Dec 2021 17:05:11: end of X-cor INFO @ Sat, 11 Dec 2021 17:05:11: #2 finished! INFO @ Sat, 11 Dec 2021 17:05:11: #2 predicted fragment length is 286 bps INFO @ Sat, 11 Dec 2021 17:05:11: #2 alternative fragment length(s) may be 286 bps INFO @ Sat, 11 Dec 2021 17:05:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.05_model.r INFO @ Sat, 11 Dec 2021 17:05:11: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:05:11: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 17:05:15: 5000000 INFO @ Sat, 11 Dec 2021 17:05:23: 7000000 INFO @ Sat, 11 Dec 2021 17:05:30: 6000000 INFO @ Sat, 11 Dec 2021 17:05:37: 8000000 INFO @ Sat, 11 Dec 2021 17:05:40: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 17:05:40: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 17:05:40: #1 total tags in treatment: 8216276 INFO @ Sat, 11 Dec 2021 17:05:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:05:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:05:41: #1 tags after filtering in treatment: 8215864 INFO @ Sat, 11 Dec 2021 17:05:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:05:41: #1 finished! INFO @ Sat, 11 Dec 2021 17:05:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:05:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:05:42: #2 number of paired peaks: 9331 INFO @ Sat, 11 Dec 2021 17:05:42: start model_add_line... INFO @ Sat, 11 Dec 2021 17:05:43: start X-correlation... INFO @ Sat, 11 Dec 2021 17:05:43: end of X-cor INFO @ Sat, 11 Dec 2021 17:05:43: #2 finished! INFO @ Sat, 11 Dec 2021 17:05:43: #2 predicted fragment length is 286 bps INFO @ Sat, 11 Dec 2021 17:05:43: #2 alternative fragment length(s) may be 286 bps INFO @ Sat, 11 Dec 2021 17:05:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.10_model.r INFO @ Sat, 11 Dec 2021 17:05:43: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:05:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:05:45: 7000000 INFO @ Sat, 11 Dec 2021 17:05:56: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:05:59: 8000000 INFO @ Sat, 11 Dec 2021 17:06:02: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 17:06:02: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 17:06:02: #1 total tags in treatment: 8216276 INFO @ Sat, 11 Dec 2021 17:06:02: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 17:06:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 17:06:03: #1 tags after filtering in treatment: 8215864 INFO @ Sat, 11 Dec 2021 17:06:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 17:06:03: #1 finished! INFO @ Sat, 11 Dec 2021 17:06:03: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 17:06:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 17:06:04: #2 number of paired peaks: 9331 INFO @ Sat, 11 Dec 2021 17:06:04: start model_add_line... INFO @ Sat, 11 Dec 2021 17:06:04: start X-correlation... INFO @ Sat, 11 Dec 2021 17:06:04: end of X-cor INFO @ Sat, 11 Dec 2021 17:06:04: #2 finished! INFO @ Sat, 11 Dec 2021 17:06:04: #2 predicted fragment length is 286 bps INFO @ Sat, 11 Dec 2021 17:06:04: #2 alternative fragment length(s) may be 286 bps INFO @ Sat, 11 Dec 2021 17:06:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.20_model.r INFO @ Sat, 11 Dec 2021 17:06:04: #3 Call peaks... INFO @ Sat, 11 Dec 2021 17:06:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 17:06:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.05_peaks.xls INFO @ Sat, 11 Dec 2021 17:06:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:06:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.05_summits.bed INFO @ Sat, 11 Dec 2021 17:06:12: Done! pass1 - making usageList (44 chroms): 4 millis pass2 - checking and writing primary data (8337 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:06:27: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:06:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.10_peaks.xls INFO @ Sat, 11 Dec 2021 17:06:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:06:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.10_summits.bed INFO @ Sat, 11 Dec 2021 17:06:42: Done! pass1 - making usageList (36 chroms): 3 millis pass2 - checking and writing primary data (6848 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 17:06:51: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 17:07:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.20_peaks.xls INFO @ Sat, 11 Dec 2021 17:07:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 17:07:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3293005/SRX3293005.20_summits.bed INFO @ Sat, 11 Dec 2021 17:07:07: Done! pass1 - making usageList (25 chroms): 2 millis pass2 - checking and writing primary data (5455 records, 4 fields): 47 millis CompletedMACS2peakCalling