Job ID = 14172434 SRX = SRX3292997 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:56 26942356 reads; of these: 26942356 (100.00%) were unpaired; of these: 10456499 (38.81%) aligned 0 times 14692995 (54.53%) aligned exactly 1 time 1792862 (6.65%) aligned >1 times 61.19% overall alignment rate Time searching: 00:08:56 Overall time: 00:08:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2767641 / 16485857 = 0.1679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:39:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:39:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:39:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:39:56: 1000000 INFO @ Sat, 11 Dec 2021 15:40:02: 2000000 INFO @ Sat, 11 Dec 2021 15:40:08: 3000000 INFO @ Sat, 11 Dec 2021 15:40:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:40:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:40:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:40:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:40:21: 5000000 INFO @ Sat, 11 Dec 2021 15:40:28: 1000000 INFO @ Sat, 11 Dec 2021 15:40:29: 6000000 INFO @ Sat, 11 Dec 2021 15:40:36: 7000000 INFO @ Sat, 11 Dec 2021 15:40:37: 2000000 INFO @ Sat, 11 Dec 2021 15:40:44: 8000000 INFO @ Sat, 11 Dec 2021 15:40:45: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:40:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:40:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:40:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:40:51: 9000000 INFO @ Sat, 11 Dec 2021 15:40:54: 4000000 INFO @ Sat, 11 Dec 2021 15:40:57: 1000000 INFO @ Sat, 11 Dec 2021 15:40:59: 10000000 INFO @ Sat, 11 Dec 2021 15:41:03: 5000000 INFO @ Sat, 11 Dec 2021 15:41:05: 2000000 INFO @ Sat, 11 Dec 2021 15:41:07: 11000000 INFO @ Sat, 11 Dec 2021 15:41:12: 6000000 INFO @ Sat, 11 Dec 2021 15:41:12: 3000000 INFO @ Sat, 11 Dec 2021 15:41:14: 12000000 INFO @ Sat, 11 Dec 2021 15:41:20: 4000000 INFO @ Sat, 11 Dec 2021 15:41:21: 7000000 INFO @ Sat, 11 Dec 2021 15:41:22: 13000000 INFO @ Sat, 11 Dec 2021 15:41:27: 5000000 INFO @ Sat, 11 Dec 2021 15:41:28: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 15:41:28: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 15:41:28: #1 total tags in treatment: 13718216 INFO @ Sat, 11 Dec 2021 15:41:28: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:41:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:41:28: #1 tags after filtering in treatment: 13718026 INFO @ Sat, 11 Dec 2021 15:41:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:41:28: #1 finished! INFO @ Sat, 11 Dec 2021 15:41:28: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:41:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:41:29: #2 number of paired peaks: 3424 INFO @ Sat, 11 Dec 2021 15:41:29: start model_add_line... INFO @ Sat, 11 Dec 2021 15:41:29: start X-correlation... INFO @ Sat, 11 Dec 2021 15:41:29: end of X-cor INFO @ Sat, 11 Dec 2021 15:41:29: #2 finished! INFO @ Sat, 11 Dec 2021 15:41:29: #2 predicted fragment length is 253 bps INFO @ Sat, 11 Dec 2021 15:41:29: #2 alternative fragment length(s) may be 253 bps INFO @ Sat, 11 Dec 2021 15:41:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.05_model.r INFO @ Sat, 11 Dec 2021 15:41:29: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:41:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:41:30: 8000000 INFO @ Sat, 11 Dec 2021 15:41:35: 6000000 INFO @ Sat, 11 Dec 2021 15:41:39: 9000000 INFO @ Sat, 11 Dec 2021 15:41:43: 7000000 INFO @ Sat, 11 Dec 2021 15:41:47: 10000000 INFO @ Sat, 11 Dec 2021 15:41:50: 8000000 INFO @ Sat, 11 Dec 2021 15:41:56: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:41:58: 9000000 INFO @ Sat, 11 Dec 2021 15:42:05: 12000000 INFO @ Sat, 11 Dec 2021 15:42:05: 10000000 INFO @ Sat, 11 Dec 2021 15:42:07: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:42:13: 13000000 INFO @ Sat, 11 Dec 2021 15:42:13: 11000000 INFO @ Sat, 11 Dec 2021 15:42:19: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 15:42:19: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 15:42:19: #1 total tags in treatment: 13718216 INFO @ Sat, 11 Dec 2021 15:42:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:20: #1 tags after filtering in treatment: 13718026 INFO @ Sat, 11 Dec 2021 15:42:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:20: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:21: 12000000 INFO @ Sat, 11 Dec 2021 15:42:21: #2 number of paired peaks: 3424 INFO @ Sat, 11 Dec 2021 15:42:21: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:21: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:21: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:21: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:21: #2 predicted fragment length is 253 bps INFO @ Sat, 11 Dec 2021 15:42:21: #2 alternative fragment length(s) may be 253 bps INFO @ Sat, 11 Dec 2021 15:42:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.10_model.r INFO @ Sat, 11 Dec 2021 15:42:21: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:42:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:42:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:42:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.05_summits.bed INFO @ Sat, 11 Dec 2021 15:42:23: Done! BigWig に変換しました。 pass1 - making usageList (131 chroms): 2 millis pass2 - checking and writing primary data (9094 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:42:28: 13000000 INFO @ Sat, 11 Dec 2021 15:42:33: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 15:42:33: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 15:42:33: #1 total tags in treatment: 13718216 INFO @ Sat, 11 Dec 2021 15:42:33: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:33: #1 tags after filtering in treatment: 13718026 INFO @ Sat, 11 Dec 2021 15:42:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:33: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:33: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:35: #2 number of paired peaks: 3424 INFO @ Sat, 11 Dec 2021 15:42:35: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:35: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:35: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:35: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:35: #2 predicted fragment length is 253 bps INFO @ Sat, 11 Dec 2021 15:42:35: #2 alternative fragment length(s) may be 253 bps INFO @ Sat, 11 Dec 2021 15:42:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.20_model.r INFO @ Sat, 11 Dec 2021 15:42:35: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:42:58: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:43:11: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:43:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.10_summits.bed INFO @ Sat, 11 Dec 2021 15:43:14: Done! pass1 - making usageList (107 chroms): 2 millis pass2 - checking and writing primary data (6887 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:43:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3292997/SRX3292997.20_summits.bed INFO @ Sat, 11 Dec 2021 15:43:27: Done! pass1 - making usageList (90 chroms): 1 millis pass2 - checking and writing primary data (4825 records, 4 fields): 9 millis CompletedMACS2peakCalling