Job ID = 14172417 SRX = SRX3292996 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:51 25606846 reads; of these: 25606846 (100.00%) were unpaired; of these: 1415717 (5.53%) aligned 0 times 22182713 (86.63%) aligned exactly 1 time 2008416 (7.84%) aligned >1 times 94.47% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7324074 / 24191129 = 0.3028 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:39:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:39:43: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:39:43: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:39:50: 1000000 INFO @ Sat, 11 Dec 2021 15:39:58: 2000000 INFO @ Sat, 11 Dec 2021 15:40:06: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:40:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:40:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:40:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:40:13: 4000000 INFO @ Sat, 11 Dec 2021 15:40:21: 1000000 INFO @ Sat, 11 Dec 2021 15:40:22: 5000000 INFO @ Sat, 11 Dec 2021 15:40:29: 2000000 INFO @ Sat, 11 Dec 2021 15:40:31: 6000000 INFO @ Sat, 11 Dec 2021 15:40:38: 3000000 INFO @ Sat, 11 Dec 2021 15:40:39: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:40:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:40:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:40:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:40:47: 4000000 INFO @ Sat, 11 Dec 2021 15:40:48: 8000000 INFO @ Sat, 11 Dec 2021 15:40:52: 1000000 INFO @ Sat, 11 Dec 2021 15:40:55: 5000000 INFO @ Sat, 11 Dec 2021 15:40:57: 9000000 INFO @ Sat, 11 Dec 2021 15:41:00: 2000000 INFO @ Sat, 11 Dec 2021 15:41:04: 6000000 INFO @ Sat, 11 Dec 2021 15:41:05: 10000000 INFO @ Sat, 11 Dec 2021 15:41:09: 3000000 INFO @ Sat, 11 Dec 2021 15:41:13: 7000000 INFO @ Sat, 11 Dec 2021 15:41:14: 11000000 INFO @ Sat, 11 Dec 2021 15:41:17: 4000000 INFO @ Sat, 11 Dec 2021 15:41:21: 8000000 INFO @ Sat, 11 Dec 2021 15:41:23: 12000000 INFO @ Sat, 11 Dec 2021 15:41:26: 5000000 INFO @ Sat, 11 Dec 2021 15:41:30: 9000000 INFO @ Sat, 11 Dec 2021 15:41:31: 13000000 INFO @ Sat, 11 Dec 2021 15:41:34: 6000000 INFO @ Sat, 11 Dec 2021 15:41:39: 10000000 INFO @ Sat, 11 Dec 2021 15:41:41: 14000000 INFO @ Sat, 11 Dec 2021 15:41:43: 7000000 INFO @ Sat, 11 Dec 2021 15:41:48: 11000000 INFO @ Sat, 11 Dec 2021 15:41:49: 15000000 INFO @ Sat, 11 Dec 2021 15:41:52: 8000000 INFO @ Sat, 11 Dec 2021 15:41:56: 12000000 INFO @ Sat, 11 Dec 2021 15:41:59: 16000000 INFO @ Sat, 11 Dec 2021 15:42:00: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:42:05: 13000000 INFO @ Sat, 11 Dec 2021 15:42:07: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 15:42:07: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 15:42:07: #1 total tags in treatment: 16867055 INFO @ Sat, 11 Dec 2021 15:42:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:08: #1 tags after filtering in treatment: 16866860 INFO @ Sat, 11 Dec 2021 15:42:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:08: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:09: 10000000 INFO @ Sat, 11 Dec 2021 15:42:09: #2 number of paired peaks: 5286 INFO @ Sat, 11 Dec 2021 15:42:09: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:10: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:10: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:10: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:10: #2 predicted fragment length is 262 bps INFO @ Sat, 11 Dec 2021 15:42:10: #2 alternative fragment length(s) may be 262 bps INFO @ Sat, 11 Dec 2021 15:42:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.05_model.r INFO @ Sat, 11 Dec 2021 15:42:10: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:42:14: 14000000 INFO @ Sat, 11 Dec 2021 15:42:18: 11000000 INFO @ Sat, 11 Dec 2021 15:42:23: 15000000 INFO @ Sat, 11 Dec 2021 15:42:26: 12000000 INFO @ Sat, 11 Dec 2021 15:42:32: 16000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:42:35: 13000000 INFO @ Sat, 11 Dec 2021 15:42:40: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 15:42:40: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 15:42:40: #1 total tags in treatment: 16867055 INFO @ Sat, 11 Dec 2021 15:42:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:42:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:42:41: #1 tags after filtering in treatment: 16866860 INFO @ Sat, 11 Dec 2021 15:42:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:42:41: #1 finished! INFO @ Sat, 11 Dec 2021 15:42:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:42:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:42:42: #2 number of paired peaks: 5286 INFO @ Sat, 11 Dec 2021 15:42:42: start model_add_line... INFO @ Sat, 11 Dec 2021 15:42:43: start X-correlation... INFO @ Sat, 11 Dec 2021 15:42:43: end of X-cor INFO @ Sat, 11 Dec 2021 15:42:43: #2 finished! INFO @ Sat, 11 Dec 2021 15:42:43: #2 predicted fragment length is 262 bps INFO @ Sat, 11 Dec 2021 15:42:43: #2 alternative fragment length(s) may be 262 bps INFO @ Sat, 11 Dec 2021 15:42:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.10_model.r INFO @ Sat, 11 Dec 2021 15:42:43: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:42:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:42:43: 14000000 INFO @ Sat, 11 Dec 2021 15:42:51: 15000000 INFO @ Sat, 11 Dec 2021 15:42:59: 16000000 INFO @ Sat, 11 Dec 2021 15:43:01: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:43:06: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 15:43:06: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 15:43:06: #1 total tags in treatment: 16867055 INFO @ Sat, 11 Dec 2021 15:43:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:43:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:43:07: #1 tags after filtering in treatment: 16866860 INFO @ Sat, 11 Dec 2021 15:43:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:43:07: #1 finished! INFO @ Sat, 11 Dec 2021 15:43:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:43:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:43:09: #2 number of paired peaks: 5286 INFO @ Sat, 11 Dec 2021 15:43:09: start model_add_line... INFO @ Sat, 11 Dec 2021 15:43:09: start X-correlation... INFO @ Sat, 11 Dec 2021 15:43:09: end of X-cor INFO @ Sat, 11 Dec 2021 15:43:09: #2 finished! INFO @ Sat, 11 Dec 2021 15:43:09: #2 predicted fragment length is 262 bps INFO @ Sat, 11 Dec 2021 15:43:09: #2 alternative fragment length(s) may be 262 bps INFO @ Sat, 11 Dec 2021 15:43:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.20_model.r INFO @ Sat, 11 Dec 2021 15:43:09: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:43:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:43:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.05_summits.bed INFO @ Sat, 11 Dec 2021 15:43:23: Done! pass1 - making usageList (128 chroms): 2 millis pass2 - checking and writing primary data (11260 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:43:34: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:43:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:43:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:43:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.10_summits.bed INFO @ Sat, 11 Dec 2021 15:43:58: Done! pass1 - making usageList (109 chroms): 2 millis pass2 - checking and writing primary data (9140 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:43:59: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:44:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:44:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:44:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3292996/SRX3292996.20_summits.bed INFO @ Sat, 11 Dec 2021 15:44:21: Done! pass1 - making usageList (87 chroms): 2 millis pass2 - checking and writing primary data (6865 records, 4 fields): 10 millis CompletedMACS2peakCalling