Job ID = 6456177 SRX = SRX3279765 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:05:54 prefetch.2.10.7: 1) Downloading 'SRR6168915'... 2020-06-21T11:05:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:09:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:09:19 prefetch.2.10.7: 1) 'SRR6168915' was downloaded successfully 2020-06-21T11:09:19 prefetch.2.10.7: 'SRR6168915' has 0 unresolved dependencies Read 39352844 spots for SRR6168915/SRR6168915.sra Written 39352844 spots for SRR6168915/SRR6168915.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:08 39352844 reads; of these: 39352844 (100.00%) were unpaired; of these: 1148190 (2.92%) aligned 0 times 34364117 (87.32%) aligned exactly 1 time 3840537 (9.76%) aligned >1 times 97.08% overall alignment rate Time searching: 00:08:08 Overall time: 00:08:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5256541 / 38204654 = 0.1376 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:18: 1000000 INFO @ Sun, 21 Jun 2020 20:29:24: 2000000 INFO @ Sun, 21 Jun 2020 20:29:29: 3000000 INFO @ Sun, 21 Jun 2020 20:29:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:41: 5000000 INFO @ Sun, 21 Jun 2020 20:29:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:47: 6000000 INFO @ Sun, 21 Jun 2020 20:29:49: 1000000 INFO @ Sun, 21 Jun 2020 20:29:54: 7000000 INFO @ Sun, 21 Jun 2020 20:29:55: 2000000 INFO @ Sun, 21 Jun 2020 20:30:00: 8000000 INFO @ Sun, 21 Jun 2020 20:30:02: 3000000 INFO @ Sun, 21 Jun 2020 20:30:07: 9000000 INFO @ Sun, 21 Jun 2020 20:30:09: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:30:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:30:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:30:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:30:13: 10000000 INFO @ Sun, 21 Jun 2020 20:30:15: 5000000 INFO @ Sun, 21 Jun 2020 20:30:19: 1000000 INFO @ Sun, 21 Jun 2020 20:30:20: 11000000 INFO @ Sun, 21 Jun 2020 20:30:22: 6000000 INFO @ Sun, 21 Jun 2020 20:30:26: 2000000 INFO @ Sun, 21 Jun 2020 20:30:26: 12000000 INFO @ Sun, 21 Jun 2020 20:30:29: 7000000 INFO @ Sun, 21 Jun 2020 20:30:32: 3000000 INFO @ Sun, 21 Jun 2020 20:30:33: 13000000 INFO @ Sun, 21 Jun 2020 20:30:35: 8000000 INFO @ Sun, 21 Jun 2020 20:30:39: 4000000 INFO @ Sun, 21 Jun 2020 20:30:40: 14000000 INFO @ Sun, 21 Jun 2020 20:30:42: 9000000 INFO @ Sun, 21 Jun 2020 20:30:46: 15000000 INFO @ Sun, 21 Jun 2020 20:30:46: 5000000 INFO @ Sun, 21 Jun 2020 20:30:49: 10000000 INFO @ Sun, 21 Jun 2020 20:30:53: 16000000 INFO @ Sun, 21 Jun 2020 20:30:53: 6000000 INFO @ Sun, 21 Jun 2020 20:30:55: 11000000 INFO @ Sun, 21 Jun 2020 20:30:59: 17000000 INFO @ Sun, 21 Jun 2020 20:31:00: 7000000 INFO @ Sun, 21 Jun 2020 20:31:02: 12000000 INFO @ Sun, 21 Jun 2020 20:31:06: 18000000 INFO @ Sun, 21 Jun 2020 20:31:06: 8000000 INFO @ Sun, 21 Jun 2020 20:31:09: 13000000 INFO @ Sun, 21 Jun 2020 20:31:12: 19000000 INFO @ Sun, 21 Jun 2020 20:31:13: 9000000 INFO @ Sun, 21 Jun 2020 20:31:15: 14000000 INFO @ Sun, 21 Jun 2020 20:31:19: 20000000 INFO @ Sun, 21 Jun 2020 20:31:20: 10000000 INFO @ Sun, 21 Jun 2020 20:31:22: 15000000 INFO @ Sun, 21 Jun 2020 20:31:25: 21000000 INFO @ Sun, 21 Jun 2020 20:31:26: 11000000 INFO @ Sun, 21 Jun 2020 20:31:29: 16000000 INFO @ Sun, 21 Jun 2020 20:31:32: 22000000 INFO @ Sun, 21 Jun 2020 20:31:33: 12000000 INFO @ Sun, 21 Jun 2020 20:31:35: 17000000 INFO @ Sun, 21 Jun 2020 20:31:39: 23000000 INFO @ Sun, 21 Jun 2020 20:31:40: 13000000 INFO @ Sun, 21 Jun 2020 20:31:42: 18000000 INFO @ Sun, 21 Jun 2020 20:31:45: 24000000 INFO @ Sun, 21 Jun 2020 20:31:46: 14000000 INFO @ Sun, 21 Jun 2020 20:31:48: 19000000 INFO @ Sun, 21 Jun 2020 20:31:52: 25000000 INFO @ Sun, 21 Jun 2020 20:31:53: 15000000 INFO @ Sun, 21 Jun 2020 20:31:55: 20000000 INFO @ Sun, 21 Jun 2020 20:31:59: 26000000 INFO @ Sun, 21 Jun 2020 20:31:59: 16000000 INFO @ Sun, 21 Jun 2020 20:32:02: 21000000 INFO @ Sun, 21 Jun 2020 20:32:06: 27000000 INFO @ Sun, 21 Jun 2020 20:32:06: 17000000 INFO @ Sun, 21 Jun 2020 20:32:08: 22000000 INFO @ Sun, 21 Jun 2020 20:32:12: 28000000 INFO @ Sun, 21 Jun 2020 20:32:13: 18000000 INFO @ Sun, 21 Jun 2020 20:32:15: 23000000 INFO @ Sun, 21 Jun 2020 20:32:19: 29000000 INFO @ Sun, 21 Jun 2020 20:32:19: 19000000 INFO @ Sun, 21 Jun 2020 20:32:22: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:32:25: 30000000 INFO @ Sun, 21 Jun 2020 20:32:26: 20000000 INFO @ Sun, 21 Jun 2020 20:32:28: 25000000 INFO @ Sun, 21 Jun 2020 20:32:32: 31000000 INFO @ Sun, 21 Jun 2020 20:32:33: 21000000 INFO @ Sun, 21 Jun 2020 20:32:35: 26000000 INFO @ Sun, 21 Jun 2020 20:32:39: 32000000 INFO @ Sun, 21 Jun 2020 20:32:39: 22000000 INFO @ Sun, 21 Jun 2020 20:32:42: 27000000 INFO @ Sun, 21 Jun 2020 20:32:45: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:32:45: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:32:45: #1 total tags in treatment: 32948113 INFO @ Sun, 21 Jun 2020 20:32:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:32:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:32:46: 23000000 INFO @ Sun, 21 Jun 2020 20:32:46: #1 tags after filtering in treatment: 32947994 INFO @ Sun, 21 Jun 2020 20:32:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:32:46: #1 finished! INFO @ Sun, 21 Jun 2020 20:32:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:32:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:32:48: #2 number of paired peaks: 44 WARNING @ Sun, 21 Jun 2020 20:32:48: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:32:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:32:49: 28000000 INFO @ Sun, 21 Jun 2020 20:32:53: 24000000 INFO @ Sun, 21 Jun 2020 20:32:55: 29000000 INFO @ Sun, 21 Jun 2020 20:32:59: 25000000 INFO @ Sun, 21 Jun 2020 20:33:02: 30000000 INFO @ Sun, 21 Jun 2020 20:33:06: 26000000 INFO @ Sun, 21 Jun 2020 20:33:08: 31000000 INFO @ Sun, 21 Jun 2020 20:33:13: 27000000 INFO @ Sun, 21 Jun 2020 20:33:15: 32000000 INFO @ Sun, 21 Jun 2020 20:33:19: 28000000 INFO @ Sun, 21 Jun 2020 20:33:22: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:33:22: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:33:22: #1 total tags in treatment: 32948113 INFO @ Sun, 21 Jun 2020 20:33:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:33:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:33:22: #1 tags after filtering in treatment: 32947994 INFO @ Sun, 21 Jun 2020 20:33:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:33:22: #1 finished! INFO @ Sun, 21 Jun 2020 20:33:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:33:22: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:33:24: #2 number of paired peaks: 44 WARNING @ Sun, 21 Jun 2020 20:33:24: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:33:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:33:25: 29000000 INFO @ Sun, 21 Jun 2020 20:33:32: 30000000 INFO @ Sun, 21 Jun 2020 20:33:37: 31000000 INFO @ Sun, 21 Jun 2020 20:33:43: 32000000 INFO @ Sun, 21 Jun 2020 20:33:50: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:33:50: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:33:50: #1 total tags in treatment: 32948113 INFO @ Sun, 21 Jun 2020 20:33:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:33:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:33:50: #1 tags after filtering in treatment: 32947994 INFO @ Sun, 21 Jun 2020 20:33:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:33:50: #1 finished! INFO @ Sun, 21 Jun 2020 20:33:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:33:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:33:52: #2 number of paired peaks: 44 WARNING @ Sun, 21 Jun 2020 20:33:52: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:33:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279765/SRX3279765.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling