Job ID = 6456172 SRX = SRX3279761 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:47:23 prefetch.2.10.7: 1) Downloading 'SRR6168911'... 2020-06-21T10:47:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:51:48 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:51:48 prefetch.2.10.7: 1) 'SRR6168911' was downloaded successfully 2020-06-21T10:51:48 prefetch.2.10.7: 'SRR6168911' has 0 unresolved dependencies Read 42776065 spots for SRR6168911/SRR6168911.sra Written 42776065 spots for SRR6168911/SRR6168911.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:48 42776065 reads; of these: 42776065 (100.00%) were unpaired; of these: 1520343 (3.55%) aligned 0 times 37122870 (86.78%) aligned exactly 1 time 4132852 (9.66%) aligned >1 times 96.45% overall alignment rate Time searching: 00:08:48 Overall time: 00:08:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5977226 / 41255722 = 0.1449 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:13:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:13:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:13:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:13:23: 1000000 INFO @ Sun, 21 Jun 2020 20:13:29: 2000000 INFO @ Sun, 21 Jun 2020 20:13:34: 3000000 INFO @ Sun, 21 Jun 2020 20:13:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:13:46: 5000000 INFO @ Sun, 21 Jun 2020 20:13:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:13:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:13:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:13:52: 6000000 INFO @ Sun, 21 Jun 2020 20:13:54: 1000000 INFO @ Sun, 21 Jun 2020 20:13:58: 7000000 INFO @ Sun, 21 Jun 2020 20:14:00: 2000000 INFO @ Sun, 21 Jun 2020 20:14:05: 8000000 INFO @ Sun, 21 Jun 2020 20:14:07: 3000000 INFO @ Sun, 21 Jun 2020 20:14:11: 9000000 INFO @ Sun, 21 Jun 2020 20:14:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:14:17: 10000000 INFO @ Sun, 21 Jun 2020 20:14:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:14:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:14:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:14:19: 5000000 INFO @ Sun, 21 Jun 2020 20:14:24: 11000000 INFO @ Sun, 21 Jun 2020 20:14:24: 1000000 INFO @ Sun, 21 Jun 2020 20:14:26: 6000000 INFO @ Sun, 21 Jun 2020 20:14:30: 12000000 INFO @ Sun, 21 Jun 2020 20:14:31: 2000000 INFO @ Sun, 21 Jun 2020 20:14:32: 7000000 INFO @ Sun, 21 Jun 2020 20:14:37: 13000000 INFO @ Sun, 21 Jun 2020 20:14:37: 3000000 INFO @ Sun, 21 Jun 2020 20:14:39: 8000000 INFO @ Sun, 21 Jun 2020 20:14:43: 14000000 INFO @ Sun, 21 Jun 2020 20:14:44: 4000000 INFO @ Sun, 21 Jun 2020 20:14:45: 9000000 INFO @ Sun, 21 Jun 2020 20:14:50: 15000000 INFO @ Sun, 21 Jun 2020 20:14:50: 5000000 INFO @ Sun, 21 Jun 2020 20:14:52: 10000000 INFO @ Sun, 21 Jun 2020 20:14:56: 16000000 INFO @ Sun, 21 Jun 2020 20:14:57: 6000000 INFO @ Sun, 21 Jun 2020 20:14:58: 11000000 INFO @ Sun, 21 Jun 2020 20:15:03: 17000000 INFO @ Sun, 21 Jun 2020 20:15:03: 7000000 INFO @ Sun, 21 Jun 2020 20:15:04: 12000000 INFO @ Sun, 21 Jun 2020 20:15:09: 18000000 INFO @ Sun, 21 Jun 2020 20:15:10: 8000000 INFO @ Sun, 21 Jun 2020 20:15:11: 13000000 INFO @ Sun, 21 Jun 2020 20:15:15: 19000000 INFO @ Sun, 21 Jun 2020 20:15:16: 9000000 INFO @ Sun, 21 Jun 2020 20:15:17: 14000000 INFO @ Sun, 21 Jun 2020 20:15:22: 20000000 INFO @ Sun, 21 Jun 2020 20:15:23: 10000000 INFO @ Sun, 21 Jun 2020 20:15:23: 15000000 INFO @ Sun, 21 Jun 2020 20:15:28: 21000000 INFO @ Sun, 21 Jun 2020 20:15:29: 11000000 INFO @ Sun, 21 Jun 2020 20:15:30: 16000000 INFO @ Sun, 21 Jun 2020 20:15:35: 22000000 INFO @ Sun, 21 Jun 2020 20:15:36: 12000000 INFO @ Sun, 21 Jun 2020 20:15:36: 17000000 INFO @ Sun, 21 Jun 2020 20:15:41: 23000000 INFO @ Sun, 21 Jun 2020 20:15:43: 13000000 INFO @ Sun, 21 Jun 2020 20:15:43: 18000000 INFO @ Sun, 21 Jun 2020 20:15:48: 24000000 INFO @ Sun, 21 Jun 2020 20:15:49: 14000000 INFO @ Sun, 21 Jun 2020 20:15:49: 19000000 INFO @ Sun, 21 Jun 2020 20:15:54: 25000000 INFO @ Sun, 21 Jun 2020 20:15:55: 15000000 INFO @ Sun, 21 Jun 2020 20:15:56: 20000000 INFO @ Sun, 21 Jun 2020 20:16:01: 26000000 INFO @ Sun, 21 Jun 2020 20:16:02: 16000000 INFO @ Sun, 21 Jun 2020 20:16:02: 21000000 INFO @ Sun, 21 Jun 2020 20:16:07: 27000000 INFO @ Sun, 21 Jun 2020 20:16:08: 17000000 INFO @ Sun, 21 Jun 2020 20:16:08: 22000000 INFO @ Sun, 21 Jun 2020 20:16:14: 28000000 INFO @ Sun, 21 Jun 2020 20:16:15: 18000000 INFO @ Sun, 21 Jun 2020 20:16:15: 23000000 INFO @ Sun, 21 Jun 2020 20:16:21: 29000000 INFO @ Sun, 21 Jun 2020 20:16:21: 19000000 INFO @ Sun, 21 Jun 2020 20:16:21: 24000000 INFO @ Sun, 21 Jun 2020 20:16:27: 30000000 INFO @ Sun, 21 Jun 2020 20:16:28: 20000000 INFO @ Sun, 21 Jun 2020 20:16:28: 25000000 INFO @ Sun, 21 Jun 2020 20:16:34: 31000000 INFO @ Sun, 21 Jun 2020 20:16:34: 21000000 INFO @ Sun, 21 Jun 2020 20:16:34: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:16:40: 32000000 INFO @ Sun, 21 Jun 2020 20:16:41: 22000000 INFO @ Sun, 21 Jun 2020 20:16:41: 27000000 INFO @ Sun, 21 Jun 2020 20:16:46: 33000000 INFO @ Sun, 21 Jun 2020 20:16:47: 23000000 INFO @ Sun, 21 Jun 2020 20:16:47: 28000000 INFO @ Sun, 21 Jun 2020 20:16:53: 34000000 INFO @ Sun, 21 Jun 2020 20:16:53: 24000000 INFO @ Sun, 21 Jun 2020 20:16:54: 29000000 INFO @ Sun, 21 Jun 2020 20:16:59: 35000000 INFO @ Sun, 21 Jun 2020 20:17:00: 25000000 INFO @ Sun, 21 Jun 2020 20:17:00: 30000000 INFO @ Sun, 21 Jun 2020 20:17:01: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:17:01: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:17:01: #1 total tags in treatment: 35278496 INFO @ Sun, 21 Jun 2020 20:17:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:02: #1 tags after filtering in treatment: 35278389 INFO @ Sun, 21 Jun 2020 20:17:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:02: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:04: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 20:17:04: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:17:06: 26000000 INFO @ Sun, 21 Jun 2020 20:17:06: 31000000 INFO @ Sun, 21 Jun 2020 20:17:12: 27000000 INFO @ Sun, 21 Jun 2020 20:17:13: 32000000 INFO @ Sun, 21 Jun 2020 20:17:19: 28000000 INFO @ Sun, 21 Jun 2020 20:17:19: 33000000 INFO @ Sun, 21 Jun 2020 20:17:25: 34000000 INFO @ Sun, 21 Jun 2020 20:17:25: 29000000 INFO @ Sun, 21 Jun 2020 20:17:31: 35000000 INFO @ Sun, 21 Jun 2020 20:17:32: 30000000 INFO @ Sun, 21 Jun 2020 20:17:33: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:17:33: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:17:33: #1 total tags in treatment: 35278496 INFO @ Sun, 21 Jun 2020 20:17:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:34: #1 tags after filtering in treatment: 35278389 INFO @ Sun, 21 Jun 2020 20:17:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:34: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:36: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 20:17:36: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:17:38: 31000000 INFO @ Sun, 21 Jun 2020 20:17:43: 32000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:17:49: 33000000 INFO @ Sun, 21 Jun 2020 20:17:54: 34000000 INFO @ Sun, 21 Jun 2020 20:18:00: 35000000 INFO @ Sun, 21 Jun 2020 20:18:02: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:18:02: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:18:02: #1 total tags in treatment: 35278496 INFO @ Sun, 21 Jun 2020 20:18:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:18:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:18:03: #1 tags after filtering in treatment: 35278389 INFO @ Sun, 21 Jun 2020 20:18:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:18:03: #1 finished! INFO @ Sun, 21 Jun 2020 20:18:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:18:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:18:05: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 20:18:05: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:18:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3279761/SRX3279761.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling