Job ID = 6456090 SRX = SRX316948 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:36:29 prefetch.2.10.7: 1) Downloading 'SRR924244'... 2020-06-21T10:36:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:42:34 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:42:34 prefetch.2.10.7: 1) 'SRR924244' was downloaded successfully Read 20922935 spots for SRR924244/SRR924244.sra Written 20922935 spots for SRR924244/SRR924244.sra 2020-06-21T10:43:44 prefetch.2.10.7: 1) Downloading 'SRR924245'... 2020-06-21T10:43:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:49:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:49:12 prefetch.2.10.7: 1) 'SRR924245' was downloaded successfully Read 28246963 spots for SRR924245/SRR924245.sra Written 28246963 spots for SRR924245/SRR924245.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:48 49169898 reads; of these: 49169898 (100.00%) were unpaired; of these: 10619381 (21.60%) aligned 0 times 28984001 (58.95%) aligned exactly 1 time 9566516 (19.46%) aligned >1 times 78.40% overall alignment rate Time searching: 00:10:48 Overall time: 00:10:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14295995 / 38550517 = 0.3708 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:10:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:10:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:10:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:10:24: 1000000 INFO @ Sun, 21 Jun 2020 20:10:29: 2000000 INFO @ Sun, 21 Jun 2020 20:10:35: 3000000 INFO @ Sun, 21 Jun 2020 20:10:40: 4000000 INFO @ Sun, 21 Jun 2020 20:10:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:10:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:10:49: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:10:49: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:10:50: 6000000 INFO @ Sun, 21 Jun 2020 20:10:55: 1000000 INFO @ Sun, 21 Jun 2020 20:10:56: 7000000 INFO @ Sun, 21 Jun 2020 20:11:00: 2000000 INFO @ Sun, 21 Jun 2020 20:11:01: 8000000 INFO @ Sun, 21 Jun 2020 20:11:06: 3000000 INFO @ Sun, 21 Jun 2020 20:11:07: 9000000 INFO @ Sun, 21 Jun 2020 20:11:11: 4000000 INFO @ Sun, 21 Jun 2020 20:11:13: 10000000 INFO @ Sun, 21 Jun 2020 20:11:17: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:18: 11000000 INFO @ Sun, 21 Jun 2020 20:11:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:23: 6000000 INFO @ Sun, 21 Jun 2020 20:11:24: 12000000 INFO @ Sun, 21 Jun 2020 20:11:26: 1000000 INFO @ Sun, 21 Jun 2020 20:11:29: 7000000 INFO @ Sun, 21 Jun 2020 20:11:30: 13000000 INFO @ Sun, 21 Jun 2020 20:11:32: 2000000 INFO @ Sun, 21 Jun 2020 20:11:35: 8000000 INFO @ Sun, 21 Jun 2020 20:11:36: 14000000 INFO @ Sun, 21 Jun 2020 20:11:39: 3000000 INFO @ Sun, 21 Jun 2020 20:11:41: 9000000 INFO @ Sun, 21 Jun 2020 20:11:42: 15000000 INFO @ Sun, 21 Jun 2020 20:11:46: 4000000 INFO @ Sun, 21 Jun 2020 20:11:47: 10000000 INFO @ Sun, 21 Jun 2020 20:11:47: 16000000 INFO @ Sun, 21 Jun 2020 20:11:52: 5000000 INFO @ Sun, 21 Jun 2020 20:11:53: 11000000 INFO @ Sun, 21 Jun 2020 20:11:53: 17000000 INFO @ Sun, 21 Jun 2020 20:11:58: 12000000 INFO @ Sun, 21 Jun 2020 20:11:59: 18000000 INFO @ Sun, 21 Jun 2020 20:11:59: 6000000 INFO @ Sun, 21 Jun 2020 20:12:04: 13000000 INFO @ Sun, 21 Jun 2020 20:12:05: 19000000 INFO @ Sun, 21 Jun 2020 20:12:06: 7000000 INFO @ Sun, 21 Jun 2020 20:12:10: 14000000 INFO @ Sun, 21 Jun 2020 20:12:11: 20000000 INFO @ Sun, 21 Jun 2020 20:12:13: 8000000 INFO @ Sun, 21 Jun 2020 20:12:16: 15000000 INFO @ Sun, 21 Jun 2020 20:12:17: 21000000 INFO @ Sun, 21 Jun 2020 20:12:19: 9000000 INFO @ Sun, 21 Jun 2020 20:12:22: 16000000 INFO @ Sun, 21 Jun 2020 20:12:23: 22000000 INFO @ Sun, 21 Jun 2020 20:12:26: 10000000 INFO @ Sun, 21 Jun 2020 20:12:28: 17000000 INFO @ Sun, 21 Jun 2020 20:12:29: 23000000 INFO @ Sun, 21 Jun 2020 20:12:33: 11000000 INFO @ Sun, 21 Jun 2020 20:12:33: 18000000 INFO @ Sun, 21 Jun 2020 20:12:35: 24000000 INFO @ Sun, 21 Jun 2020 20:12:37: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:12:37: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:12:37: #1 total tags in treatment: 24254522 INFO @ Sun, 21 Jun 2020 20:12:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:12:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:12:37: #1 tags after filtering in treatment: 24254522 INFO @ Sun, 21 Jun 2020 20:12:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:12:37: #1 finished! INFO @ Sun, 21 Jun 2020 20:12:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:12:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:12:39: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 20:12:39: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:12:39: 19000000 INFO @ Sun, 21 Jun 2020 20:12:39: 12000000 INFO @ Sun, 21 Jun 2020 20:12:46: 20000000 INFO @ Sun, 21 Jun 2020 20:12:46: 13000000 INFO @ Sun, 21 Jun 2020 20:12:52: 21000000 INFO @ Sun, 21 Jun 2020 20:12:52: 14000000 INFO @ Sun, 21 Jun 2020 20:12:58: 22000000 INFO @ Sun, 21 Jun 2020 20:12:59: 15000000 INFO @ Sun, 21 Jun 2020 20:13:04: 23000000 INFO @ Sun, 21 Jun 2020 20:13:05: 16000000 INFO @ Sun, 21 Jun 2020 20:13:10: 24000000 INFO @ Sun, 21 Jun 2020 20:13:11: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:13:11: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:13:11: #1 total tags in treatment: 24254522 INFO @ Sun, 21 Jun 2020 20:13:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:13:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:13:12: 17000000 INFO @ Sun, 21 Jun 2020 20:13:12: #1 tags after filtering in treatment: 24254522 INFO @ Sun, 21 Jun 2020 20:13:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:13:12: #1 finished! INFO @ Sun, 21 Jun 2020 20:13:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:13:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:13:14: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 20:13:14: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:13:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:13:18: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:13:24: 19000000 INFO @ Sun, 21 Jun 2020 20:13:31: 20000000 INFO @ Sun, 21 Jun 2020 20:13:37: 21000000 INFO @ Sun, 21 Jun 2020 20:13:43: 22000000 INFO @ Sun, 21 Jun 2020 20:13:49: 23000000 INFO @ Sun, 21 Jun 2020 20:13:56: 24000000 INFO @ Sun, 21 Jun 2020 20:13:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:13:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:13:58: #1 total tags in treatment: 24254522 INFO @ Sun, 21 Jun 2020 20:13:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:13:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:13:58: #1 tags after filtering in treatment: 24254522 INFO @ Sun, 21 Jun 2020 20:13:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:13:58: #1 finished! INFO @ Sun, 21 Jun 2020 20:13:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:13:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:14:00: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 20:14:00: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:14:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX316948/SRX316948.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。