Job ID = 6456079 SRX = SRX3167257 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:35:59 prefetch.2.10.7: 1) Downloading 'SRR6014260'... 2020-06-21T10:35:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:48:18 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:48:18 prefetch.2.10.7: 1) 'SRR6014260' was downloaded successfully Read 67471656 spots for SRR6014260/SRR6014260.sra Written 67471656 spots for SRR6014260/SRR6014260.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:29 67471656 reads; of these: 67471656 (100.00%) were unpaired; of these: 4178342 (6.19%) aligned 0 times 49037603 (72.68%) aligned exactly 1 time 14255711 (21.13%) aligned >1 times 93.81% overall alignment rate Time searching: 00:19:30 Overall time: 00:19:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13126444 / 63293314 = 0.2074 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:43: 1000000 INFO @ Sun, 21 Jun 2020 20:29:48: 2000000 INFO @ Sun, 21 Jun 2020 20:29:53: 3000000 INFO @ Sun, 21 Jun 2020 20:29:58: 4000000 INFO @ Sun, 21 Jun 2020 20:30:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:30:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:30:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:30:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:30:09: 6000000 INFO @ Sun, 21 Jun 2020 20:30:13: 1000000 INFO @ Sun, 21 Jun 2020 20:30:14: 7000000 INFO @ Sun, 21 Jun 2020 20:30:19: 2000000 INFO @ Sun, 21 Jun 2020 20:30:19: 8000000 INFO @ Sun, 21 Jun 2020 20:30:24: 3000000 INFO @ Sun, 21 Jun 2020 20:30:24: 9000000 INFO @ Sun, 21 Jun 2020 20:30:29: 4000000 INFO @ Sun, 21 Jun 2020 20:30:30: 10000000 INFO @ Sun, 21 Jun 2020 20:30:34: 5000000 INFO @ Sun, 21 Jun 2020 20:30:35: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:30:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:30:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:30:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:30:40: 6000000 INFO @ Sun, 21 Jun 2020 20:30:40: 12000000 INFO @ Sun, 21 Jun 2020 20:30:44: 1000000 INFO @ Sun, 21 Jun 2020 20:30:45: 7000000 INFO @ Sun, 21 Jun 2020 20:30:46: 13000000 INFO @ Sun, 21 Jun 2020 20:30:49: 2000000 INFO @ Sun, 21 Jun 2020 20:30:50: 8000000 INFO @ Sun, 21 Jun 2020 20:30:52: 14000000 INFO @ Sun, 21 Jun 2020 20:30:54: 3000000 INFO @ Sun, 21 Jun 2020 20:30:56: 9000000 INFO @ Sun, 21 Jun 2020 20:30:57: 15000000 INFO @ Sun, 21 Jun 2020 20:31:00: 4000000 INFO @ Sun, 21 Jun 2020 20:31:01: 10000000 INFO @ Sun, 21 Jun 2020 20:31:03: 16000000 INFO @ Sun, 21 Jun 2020 20:31:05: 5000000 INFO @ Sun, 21 Jun 2020 20:31:07: 11000000 INFO @ Sun, 21 Jun 2020 20:31:09: 17000000 INFO @ Sun, 21 Jun 2020 20:31:11: 6000000 INFO @ Sun, 21 Jun 2020 20:31:12: 12000000 INFO @ Sun, 21 Jun 2020 20:31:15: 18000000 INFO @ Sun, 21 Jun 2020 20:31:17: 7000000 INFO @ Sun, 21 Jun 2020 20:31:18: 13000000 INFO @ Sun, 21 Jun 2020 20:31:20: 19000000 INFO @ Sun, 21 Jun 2020 20:31:22: 8000000 INFO @ Sun, 21 Jun 2020 20:31:24: 14000000 INFO @ Sun, 21 Jun 2020 20:31:26: 20000000 INFO @ Sun, 21 Jun 2020 20:31:28: 9000000 INFO @ Sun, 21 Jun 2020 20:31:30: 15000000 INFO @ Sun, 21 Jun 2020 20:31:32: 21000000 INFO @ Sun, 21 Jun 2020 20:31:34: 10000000 INFO @ Sun, 21 Jun 2020 20:31:35: 16000000 INFO @ Sun, 21 Jun 2020 20:31:38: 22000000 INFO @ Sun, 21 Jun 2020 20:31:40: 11000000 INFO @ Sun, 21 Jun 2020 20:31:41: 17000000 INFO @ Sun, 21 Jun 2020 20:31:44: 23000000 INFO @ Sun, 21 Jun 2020 20:31:46: 12000000 INFO @ Sun, 21 Jun 2020 20:31:47: 18000000 INFO @ Sun, 21 Jun 2020 20:31:50: 24000000 INFO @ Sun, 21 Jun 2020 20:31:51: 13000000 INFO @ Sun, 21 Jun 2020 20:31:53: 19000000 INFO @ Sun, 21 Jun 2020 20:31:56: 25000000 INFO @ Sun, 21 Jun 2020 20:31:57: 14000000 INFO @ Sun, 21 Jun 2020 20:31:58: 20000000 INFO @ Sun, 21 Jun 2020 20:32:03: 15000000 INFO @ Sun, 21 Jun 2020 20:32:03: 26000000 INFO @ Sun, 21 Jun 2020 20:32:04: 21000000 INFO @ Sun, 21 Jun 2020 20:32:09: 16000000 INFO @ Sun, 21 Jun 2020 20:32:09: 27000000 INFO @ Sun, 21 Jun 2020 20:32:10: 22000000 INFO @ Sun, 21 Jun 2020 20:32:15: 17000000 INFO @ Sun, 21 Jun 2020 20:32:15: 23000000 INFO @ Sun, 21 Jun 2020 20:32:16: 28000000 INFO @ Sun, 21 Jun 2020 20:32:21: 18000000 INFO @ Sun, 21 Jun 2020 20:32:21: 24000000 INFO @ Sun, 21 Jun 2020 20:32:22: 29000000 INFO @ Sun, 21 Jun 2020 20:32:26: 19000000 INFO @ Sun, 21 Jun 2020 20:32:27: 25000000 INFO @ Sun, 21 Jun 2020 20:32:28: 30000000 INFO @ Sun, 21 Jun 2020 20:32:32: 20000000 INFO @ Sun, 21 Jun 2020 20:32:33: 26000000 INFO @ Sun, 21 Jun 2020 20:32:34: 31000000 INFO @ Sun, 21 Jun 2020 20:32:38: 21000000 INFO @ Sun, 21 Jun 2020 20:32:38: 27000000 INFO @ Sun, 21 Jun 2020 20:32:40: 32000000 INFO @ Sun, 21 Jun 2020 20:32:44: 28000000 INFO @ Sun, 21 Jun 2020 20:32:44: 22000000 INFO @ Sun, 21 Jun 2020 20:32:46: 33000000 INFO @ Sun, 21 Jun 2020 20:32:49: 29000000 INFO @ Sun, 21 Jun 2020 20:32:49: 23000000 INFO @ Sun, 21 Jun 2020 20:32:52: 34000000 INFO @ Sun, 21 Jun 2020 20:32:55: 30000000 INFO @ Sun, 21 Jun 2020 20:32:55: 24000000 INFO @ Sun, 21 Jun 2020 20:32:58: 35000000 INFO @ Sun, 21 Jun 2020 20:33:00: 31000000 INFO @ Sun, 21 Jun 2020 20:33:01: 25000000 INFO @ Sun, 21 Jun 2020 20:33:03: 36000000 INFO @ Sun, 21 Jun 2020 20:33:06: 32000000 INFO @ Sun, 21 Jun 2020 20:33:07: 26000000 INFO @ Sun, 21 Jun 2020 20:33:09: 37000000 INFO @ Sun, 21 Jun 2020 20:33:12: 33000000 INFO @ Sun, 21 Jun 2020 20:33:12: 27000000 INFO @ Sun, 21 Jun 2020 20:33:15: 38000000 INFO @ Sun, 21 Jun 2020 20:33:17: 34000000 INFO @ Sun, 21 Jun 2020 20:33:18: 28000000 INFO @ Sun, 21 Jun 2020 20:33:20: 39000000 INFO @ Sun, 21 Jun 2020 20:33:23: 35000000 INFO @ Sun, 21 Jun 2020 20:33:23: 29000000 INFO @ Sun, 21 Jun 2020 20:33:26: 40000000 INFO @ Sun, 21 Jun 2020 20:33:28: 36000000 INFO @ Sun, 21 Jun 2020 20:33:29: 30000000 INFO @ Sun, 21 Jun 2020 20:33:32: 41000000 INFO @ Sun, 21 Jun 2020 20:33:34: 37000000 INFO @ Sun, 21 Jun 2020 20:33:34: 31000000 INFO @ Sun, 21 Jun 2020 20:33:38: 42000000 INFO @ Sun, 21 Jun 2020 20:33:39: 38000000 INFO @ Sun, 21 Jun 2020 20:33:40: 32000000 INFO @ Sun, 21 Jun 2020 20:33:43: 43000000 INFO @ Sun, 21 Jun 2020 20:33:45: 39000000 INFO @ Sun, 21 Jun 2020 20:33:45: 33000000 INFO @ Sun, 21 Jun 2020 20:33:49: 44000000 INFO @ Sun, 21 Jun 2020 20:33:50: 40000000 INFO @ Sun, 21 Jun 2020 20:33:51: 34000000 INFO @ Sun, 21 Jun 2020 20:33:54: 45000000 INFO @ Sun, 21 Jun 2020 20:33:56: 35000000 INFO @ Sun, 21 Jun 2020 20:33:56: 41000000 INFO @ Sun, 21 Jun 2020 20:34:00: 46000000 INFO @ Sun, 21 Jun 2020 20:34:02: 36000000 INFO @ Sun, 21 Jun 2020 20:34:02: 42000000 INFO @ Sun, 21 Jun 2020 20:34:05: 47000000 INFO @ Sun, 21 Jun 2020 20:34:07: 37000000 INFO @ Sun, 21 Jun 2020 20:34:07: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:34:11: 48000000 INFO @ Sun, 21 Jun 2020 20:34:12: 44000000 INFO @ Sun, 21 Jun 2020 20:34:12: 38000000 INFO @ Sun, 21 Jun 2020 20:34:16: 49000000 INFO @ Sun, 21 Jun 2020 20:34:18: 39000000 INFO @ Sun, 21 Jun 2020 20:34:18: 45000000 INFO @ Sun, 21 Jun 2020 20:34:22: 50000000 INFO @ Sun, 21 Jun 2020 20:34:23: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:34:23: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:34:23: #1 total tags in treatment: 50166870 INFO @ Sun, 21 Jun 2020 20:34:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:34:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:34:23: 46000000 INFO @ Sun, 21 Jun 2020 20:34:23: 40000000 INFO @ Sun, 21 Jun 2020 20:34:24: #1 tags after filtering in treatment: 50166837 INFO @ Sun, 21 Jun 2020 20:34:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:34:24: #1 finished! INFO @ Sun, 21 Jun 2020 20:34:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:34:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:34:27: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:34:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:34:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:34:29: 47000000 INFO @ Sun, 21 Jun 2020 20:34:29: 41000000 INFO @ Sun, 21 Jun 2020 20:34:34: 42000000 INFO @ Sun, 21 Jun 2020 20:34:34: 48000000 INFO @ Sun, 21 Jun 2020 20:34:39: 43000000 INFO @ Sun, 21 Jun 2020 20:34:39: 49000000 INFO @ Sun, 21 Jun 2020 20:34:45: 44000000 INFO @ Sun, 21 Jun 2020 20:34:45: 50000000 INFO @ Sun, 21 Jun 2020 20:34:47: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:34:47: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:34:47: #1 total tags in treatment: 50166870 INFO @ Sun, 21 Jun 2020 20:34:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:34:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:34:48: #1 tags after filtering in treatment: 50166837 INFO @ Sun, 21 Jun 2020 20:34:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:34:48: #1 finished! INFO @ Sun, 21 Jun 2020 20:34:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:34:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:34:50: 45000000 INFO @ Sun, 21 Jun 2020 20:34:51: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:34:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:34:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:34:55: 46000000 INFO @ Sun, 21 Jun 2020 20:35:00: 47000000 INFO @ Sun, 21 Jun 2020 20:35:06: 48000000 INFO @ Sun, 21 Jun 2020 20:35:11: 49000000 INFO @ Sun, 21 Jun 2020 20:35:16: 50000000 INFO @ Sun, 21 Jun 2020 20:35:17: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:35:17: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:35:17: #1 total tags in treatment: 50166870 INFO @ Sun, 21 Jun 2020 20:35:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:35:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:35:18: #1 tags after filtering in treatment: 50166837 INFO @ Sun, 21 Jun 2020 20:35:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:35:18: #1 finished! INFO @ Sun, 21 Jun 2020 20:35:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:35:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:35:21: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:35:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:35:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3167257/SRX3167257.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。