Job ID = 6456051 SRX = SRX3166313 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:48:31 prefetch.2.10.7: 1) Downloading 'SRR6012930'... 2020-06-21T10:48:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:52:38 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:52:38 prefetch.2.10.7: 1) 'SRR6012930' was downloaded successfully 2020-06-21T10:52:38 prefetch.2.10.7: 'SRR6012930' has 0 unresolved dependencies Read 52409949 spots for SRR6012930/SRR6012930.sra Written 52409949 spots for SRR6012930/SRR6012930.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:02 52409949 reads; of these: 52409949 (100.00%) were unpaired; of these: 2021061 (3.86%) aligned 0 times 41673848 (79.52%) aligned exactly 1 time 8715040 (16.63%) aligned >1 times 96.14% overall alignment rate Time searching: 00:13:02 Overall time: 00:13:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 7498264 / 50388888 = 0.1488 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:16:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:16:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:16:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:17:03: 1000000 INFO @ Sun, 21 Jun 2020 20:17:08: 2000000 INFO @ Sun, 21 Jun 2020 20:17:13: 3000000 INFO @ Sun, 21 Jun 2020 20:17:18: 4000000 INFO @ Sun, 21 Jun 2020 20:17:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:17:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:17:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:17:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:17:29: 6000000 INFO @ Sun, 21 Jun 2020 20:17:32: 1000000 INFO @ Sun, 21 Jun 2020 20:17:34: 7000000 INFO @ Sun, 21 Jun 2020 20:17:38: 2000000 INFO @ Sun, 21 Jun 2020 20:17:39: 8000000 INFO @ Sun, 21 Jun 2020 20:17:43: 3000000 INFO @ Sun, 21 Jun 2020 20:17:44: 9000000 INFO @ Sun, 21 Jun 2020 20:17:48: 4000000 INFO @ Sun, 21 Jun 2020 20:17:50: 10000000 INFO @ Sun, 21 Jun 2020 20:17:53: 5000000 INFO @ Sun, 21 Jun 2020 20:17:55: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:17:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:17:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:17:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:17:58: 6000000 INFO @ Sun, 21 Jun 2020 20:18:00: 12000000 INFO @ Sun, 21 Jun 2020 20:18:03: 1000000 INFO @ Sun, 21 Jun 2020 20:18:03: 7000000 INFO @ Sun, 21 Jun 2020 20:18:05: 13000000 INFO @ Sun, 21 Jun 2020 20:18:08: 2000000 INFO @ Sun, 21 Jun 2020 20:18:09: 8000000 INFO @ Sun, 21 Jun 2020 20:18:11: 14000000 INFO @ Sun, 21 Jun 2020 20:18:13: 3000000 INFO @ Sun, 21 Jun 2020 20:18:14: 9000000 INFO @ Sun, 21 Jun 2020 20:18:16: 15000000 INFO @ Sun, 21 Jun 2020 20:18:18: 4000000 INFO @ Sun, 21 Jun 2020 20:18:19: 10000000 INFO @ Sun, 21 Jun 2020 20:18:21: 16000000 INFO @ Sun, 21 Jun 2020 20:18:24: 5000000 INFO @ Sun, 21 Jun 2020 20:18:24: 11000000 INFO @ Sun, 21 Jun 2020 20:18:26: 17000000 INFO @ Sun, 21 Jun 2020 20:18:29: 6000000 INFO @ Sun, 21 Jun 2020 20:18:29: 12000000 INFO @ Sun, 21 Jun 2020 20:18:32: 18000000 INFO @ Sun, 21 Jun 2020 20:18:34: 7000000 INFO @ Sun, 21 Jun 2020 20:18:35: 13000000 INFO @ Sun, 21 Jun 2020 20:18:38: 19000000 INFO @ Sun, 21 Jun 2020 20:18:39: 8000000 INFO @ Sun, 21 Jun 2020 20:18:40: 14000000 INFO @ Sun, 21 Jun 2020 20:18:43: 20000000 INFO @ Sun, 21 Jun 2020 20:18:45: 9000000 INFO @ Sun, 21 Jun 2020 20:18:45: 15000000 INFO @ Sun, 21 Jun 2020 20:18:49: 21000000 INFO @ Sun, 21 Jun 2020 20:18:50: 10000000 INFO @ Sun, 21 Jun 2020 20:18:50: 16000000 INFO @ Sun, 21 Jun 2020 20:18:54: 22000000 INFO @ Sun, 21 Jun 2020 20:18:55: 11000000 INFO @ Sun, 21 Jun 2020 20:18:55: 17000000 INFO @ Sun, 21 Jun 2020 20:19:00: 23000000 INFO @ Sun, 21 Jun 2020 20:19:00: 12000000 INFO @ Sun, 21 Jun 2020 20:19:01: 18000000 INFO @ Sun, 21 Jun 2020 20:19:05: 24000000 INFO @ Sun, 21 Jun 2020 20:19:05: 13000000 INFO @ Sun, 21 Jun 2020 20:19:06: 19000000 INFO @ Sun, 21 Jun 2020 20:19:11: 14000000 INFO @ Sun, 21 Jun 2020 20:19:11: 25000000 INFO @ Sun, 21 Jun 2020 20:19:11: 20000000 INFO @ Sun, 21 Jun 2020 20:19:16: 15000000 INFO @ Sun, 21 Jun 2020 20:19:16: 26000000 INFO @ Sun, 21 Jun 2020 20:19:17: 21000000 INFO @ Sun, 21 Jun 2020 20:19:21: 16000000 INFO @ Sun, 21 Jun 2020 20:19:22: 27000000 INFO @ Sun, 21 Jun 2020 20:19:22: 22000000 INFO @ Sun, 21 Jun 2020 20:19:26: 17000000 INFO @ Sun, 21 Jun 2020 20:19:27: 23000000 INFO @ Sun, 21 Jun 2020 20:19:27: 28000000 INFO @ Sun, 21 Jun 2020 20:19:32: 18000000 INFO @ Sun, 21 Jun 2020 20:19:33: 24000000 INFO @ Sun, 21 Jun 2020 20:19:33: 29000000 INFO @ Sun, 21 Jun 2020 20:19:37: 19000000 INFO @ Sun, 21 Jun 2020 20:19:38: 25000000 INFO @ Sun, 21 Jun 2020 20:19:38: 30000000 INFO @ Sun, 21 Jun 2020 20:19:43: 20000000 INFO @ Sun, 21 Jun 2020 20:19:44: 26000000 INFO @ Sun, 21 Jun 2020 20:19:44: 31000000 INFO @ Sun, 21 Jun 2020 20:19:48: 21000000 INFO @ Sun, 21 Jun 2020 20:19:49: 27000000 INFO @ Sun, 21 Jun 2020 20:19:49: 32000000 INFO @ Sun, 21 Jun 2020 20:19:53: 22000000 INFO @ Sun, 21 Jun 2020 20:19:54: 28000000 INFO @ Sun, 21 Jun 2020 20:19:55: 33000000 INFO @ Sun, 21 Jun 2020 20:19:59: 23000000 INFO @ Sun, 21 Jun 2020 20:20:00: 29000000 INFO @ Sun, 21 Jun 2020 20:20:00: 34000000 INFO @ Sun, 21 Jun 2020 20:20:04: 24000000 INFO @ Sun, 21 Jun 2020 20:20:05: 30000000 INFO @ Sun, 21 Jun 2020 20:20:06: 35000000 INFO @ Sun, 21 Jun 2020 20:20:10: 25000000 INFO @ Sun, 21 Jun 2020 20:20:11: 31000000 INFO @ Sun, 21 Jun 2020 20:20:11: 36000000 INFO @ Sun, 21 Jun 2020 20:20:15: 26000000 INFO @ Sun, 21 Jun 2020 20:20:16: 32000000 INFO @ Sun, 21 Jun 2020 20:20:17: 37000000 INFO @ Sun, 21 Jun 2020 20:20:20: 27000000 INFO @ Sun, 21 Jun 2020 20:20:21: 33000000 INFO @ Sun, 21 Jun 2020 20:20:22: 38000000 INFO @ Sun, 21 Jun 2020 20:20:26: 28000000 INFO @ Sun, 21 Jun 2020 20:20:27: 34000000 INFO @ Sun, 21 Jun 2020 20:20:27: 39000000 INFO @ Sun, 21 Jun 2020 20:20:31: 29000000 INFO @ Sun, 21 Jun 2020 20:20:32: 35000000 INFO @ Sun, 21 Jun 2020 20:20:33: 40000000 INFO @ Sun, 21 Jun 2020 20:20:36: 30000000 INFO @ Sun, 21 Jun 2020 20:20:38: 36000000 INFO @ Sun, 21 Jun 2020 20:20:38: 41000000 INFO @ Sun, 21 Jun 2020 20:20:42: 31000000 INFO @ Sun, 21 Jun 2020 20:20:43: 37000000 INFO @ Sun, 21 Jun 2020 20:20:44: 42000000 INFO @ Sun, 21 Jun 2020 20:20:47: 32000000 INFO @ Sun, 21 Jun 2020 20:20:48: 38000000 INFO @ Sun, 21 Jun 2020 20:20:49: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:20:49: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:20:49: #1 total tags in treatment: 42890624 INFO @ Sun, 21 Jun 2020 20:20:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:20:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:20:50: #1 tags after filtering in treatment: 42890570 INFO @ Sun, 21 Jun 2020 20:20:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:20:50: #1 finished! INFO @ Sun, 21 Jun 2020 20:20:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:20:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:20:53: 33000000 INFO @ Sun, 21 Jun 2020 20:20:53: #2 number of paired peaks: 84 WARNING @ Sun, 21 Jun 2020 20:20:53: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:20:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:20:54: 39000000 INFO @ Sun, 21 Jun 2020 20:20:58: 34000000 INFO @ Sun, 21 Jun 2020 20:20:59: 40000000 INFO @ Sun, 21 Jun 2020 20:21:04: 35000000 INFO @ Sun, 21 Jun 2020 20:21:05: 41000000 INFO @ Sun, 21 Jun 2020 20:21:09: 36000000 INFO @ Sun, 21 Jun 2020 20:21:10: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:21:14: 37000000 INFO @ Sun, 21 Jun 2020 20:21:15: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:21:15: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:21:15: #1 total tags in treatment: 42890624 INFO @ Sun, 21 Jun 2020 20:21:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:21:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:21:16: #1 tags after filtering in treatment: 42890570 INFO @ Sun, 21 Jun 2020 20:21:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:21:16: #1 finished! INFO @ Sun, 21 Jun 2020 20:21:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:21:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:21:19: #2 number of paired peaks: 84 WARNING @ Sun, 21 Jun 2020 20:21:19: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:21:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:21:20: 38000000 INFO @ Sun, 21 Jun 2020 20:21:25: 39000000 INFO @ Sun, 21 Jun 2020 20:21:30: 40000000 INFO @ Sun, 21 Jun 2020 20:21:36: 41000000 INFO @ Sun, 21 Jun 2020 20:21:41: 42000000 INFO @ Sun, 21 Jun 2020 20:21:46: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:21:46: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:21:46: #1 total tags in treatment: 42890624 INFO @ Sun, 21 Jun 2020 20:21:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:21:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:21:47: #1 tags after filtering in treatment: 42890570 INFO @ Sun, 21 Jun 2020 20:21:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:21:47: #1 finished! INFO @ Sun, 21 Jun 2020 20:21:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:21:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:21:50: #2 number of paired peaks: 84 WARNING @ Sun, 21 Jun 2020 20:21:50: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:21:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166313/SRX3166313.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。