Job ID = 6456050 SRX = SRX3166312 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:37:59 prefetch.2.10.7: 1) Downloading 'SRR6012929'... 2020-06-21T10:37:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:43:16 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:43:16 prefetch.2.10.7: 1) 'SRR6012929' was downloaded successfully 2020-06-21T10:43:16 prefetch.2.10.7: 'SRR6012929' has 0 unresolved dependencies Read 57825933 spots for SRR6012929/SRR6012929.sra Written 57825933 spots for SRR6012929/SRR6012929.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:50 57825933 reads; of these: 57825933 (100.00%) were unpaired; of these: 2210994 (3.82%) aligned 0 times 46139880 (79.79%) aligned exactly 1 time 9475059 (16.39%) aligned >1 times 96.18% overall alignment rate Time searching: 00:14:50 Overall time: 00:14:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 8343995 / 55614939 = 0.1500 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:11:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:11:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:11:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:11:37: 1000000 INFO @ Sun, 21 Jun 2020 20:11:43: 2000000 INFO @ Sun, 21 Jun 2020 20:11:48: 3000000 INFO @ Sun, 21 Jun 2020 20:11:54: 4000000 INFO @ Sun, 21 Jun 2020 20:11:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:12:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:12:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:12:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:06: 6000000 INFO @ Sun, 21 Jun 2020 20:12:09: 1000000 INFO @ Sun, 21 Jun 2020 20:12:12: 7000000 INFO @ Sun, 21 Jun 2020 20:12:16: 2000000 INFO @ Sun, 21 Jun 2020 20:12:19: 8000000 INFO @ Sun, 21 Jun 2020 20:12:24: 3000000 INFO @ Sun, 21 Jun 2020 20:12:26: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:12:31: 4000000 INFO @ Sun, 21 Jun 2020 20:12:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:12:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:12:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:12:32: 10000000 INFO @ Sun, 21 Jun 2020 20:12:38: 5000000 INFO @ Sun, 21 Jun 2020 20:12:38: 1000000 INFO @ Sun, 21 Jun 2020 20:12:39: 11000000 INFO @ Sun, 21 Jun 2020 20:12:45: 2000000 INFO @ Sun, 21 Jun 2020 20:12:45: 6000000 INFO @ Sun, 21 Jun 2020 20:12:45: 12000000 INFO @ Sun, 21 Jun 2020 20:12:51: 3000000 INFO @ Sun, 21 Jun 2020 20:12:52: 13000000 INFO @ Sun, 21 Jun 2020 20:12:52: 7000000 INFO @ Sun, 21 Jun 2020 20:12:58: 4000000 INFO @ Sun, 21 Jun 2020 20:12:59: 14000000 INFO @ Sun, 21 Jun 2020 20:12:59: 8000000 INFO @ Sun, 21 Jun 2020 20:13:04: 5000000 INFO @ Sun, 21 Jun 2020 20:13:06: 15000000 INFO @ Sun, 21 Jun 2020 20:13:07: 9000000 INFO @ Sun, 21 Jun 2020 20:13:11: 6000000 INFO @ Sun, 21 Jun 2020 20:13:13: 16000000 INFO @ Sun, 21 Jun 2020 20:13:14: 10000000 INFO @ Sun, 21 Jun 2020 20:13:18: 7000000 INFO @ Sun, 21 Jun 2020 20:13:20: 17000000 INFO @ Sun, 21 Jun 2020 20:13:21: 11000000 INFO @ Sun, 21 Jun 2020 20:13:25: 8000000 INFO @ Sun, 21 Jun 2020 20:13:27: 18000000 INFO @ Sun, 21 Jun 2020 20:13:28: 12000000 INFO @ Sun, 21 Jun 2020 20:13:31: 9000000 INFO @ Sun, 21 Jun 2020 20:13:33: 19000000 INFO @ Sun, 21 Jun 2020 20:13:36: 13000000 INFO @ Sun, 21 Jun 2020 20:13:38: 10000000 INFO @ Sun, 21 Jun 2020 20:13:40: 20000000 INFO @ Sun, 21 Jun 2020 20:13:43: 14000000 INFO @ Sun, 21 Jun 2020 20:13:45: 11000000 INFO @ Sun, 21 Jun 2020 20:13:47: 21000000 INFO @ Sun, 21 Jun 2020 20:13:50: 15000000 INFO @ Sun, 21 Jun 2020 20:13:52: 12000000 INFO @ Sun, 21 Jun 2020 20:13:54: 22000000 INFO @ Sun, 21 Jun 2020 20:13:57: 16000000 INFO @ Sun, 21 Jun 2020 20:13:58: 13000000 INFO @ Sun, 21 Jun 2020 20:14:01: 23000000 INFO @ Sun, 21 Jun 2020 20:14:05: 17000000 INFO @ Sun, 21 Jun 2020 20:14:05: 14000000 INFO @ Sun, 21 Jun 2020 20:14:08: 24000000 INFO @ Sun, 21 Jun 2020 20:14:11: 15000000 INFO @ Sun, 21 Jun 2020 20:14:12: 18000000 INFO @ Sun, 21 Jun 2020 20:14:14: 25000000 INFO @ Sun, 21 Jun 2020 20:14:18: 16000000 INFO @ Sun, 21 Jun 2020 20:14:19: 19000000 INFO @ Sun, 21 Jun 2020 20:14:21: 26000000 INFO @ Sun, 21 Jun 2020 20:14:24: 17000000 INFO @ Sun, 21 Jun 2020 20:14:27: 20000000 INFO @ Sun, 21 Jun 2020 20:14:28: 27000000 INFO @ Sun, 21 Jun 2020 20:14:31: 18000000 INFO @ Sun, 21 Jun 2020 20:14:34: 21000000 INFO @ Sun, 21 Jun 2020 20:14:35: 28000000 INFO @ Sun, 21 Jun 2020 20:14:38: 19000000 INFO @ Sun, 21 Jun 2020 20:14:41: 22000000 INFO @ Sun, 21 Jun 2020 20:14:42: 29000000 INFO @ Sun, 21 Jun 2020 20:14:45: 20000000 INFO @ Sun, 21 Jun 2020 20:14:48: 23000000 INFO @ Sun, 21 Jun 2020 20:14:48: 30000000 INFO @ Sun, 21 Jun 2020 20:14:51: 21000000 INFO @ Sun, 21 Jun 2020 20:14:55: 31000000 INFO @ Sun, 21 Jun 2020 20:14:56: 24000000 INFO @ Sun, 21 Jun 2020 20:14:58: 22000000 INFO @ Sun, 21 Jun 2020 20:15:02: 32000000 INFO @ Sun, 21 Jun 2020 20:15:03: 25000000 INFO @ Sun, 21 Jun 2020 20:15:05: 23000000 INFO @ Sun, 21 Jun 2020 20:15:09: 33000000 INFO @ Sun, 21 Jun 2020 20:15:11: 26000000 INFO @ Sun, 21 Jun 2020 20:15:12: 24000000 INFO @ Sun, 21 Jun 2020 20:15:15: 34000000 INFO @ Sun, 21 Jun 2020 20:15:18: 27000000 INFO @ Sun, 21 Jun 2020 20:15:18: 25000000 INFO @ Sun, 21 Jun 2020 20:15:22: 35000000 INFO @ Sun, 21 Jun 2020 20:15:25: 26000000 INFO @ Sun, 21 Jun 2020 20:15:26: 28000000 INFO @ Sun, 21 Jun 2020 20:15:29: 36000000 INFO @ Sun, 21 Jun 2020 20:15:32: 27000000 INFO @ Sun, 21 Jun 2020 20:15:33: 29000000 INFO @ Sun, 21 Jun 2020 20:15:36: 37000000 INFO @ Sun, 21 Jun 2020 20:15:39: 28000000 INFO @ Sun, 21 Jun 2020 20:15:41: 30000000 INFO @ Sun, 21 Jun 2020 20:15:43: 38000000 INFO @ Sun, 21 Jun 2020 20:15:46: 29000000 INFO @ Sun, 21 Jun 2020 20:15:49: 31000000 INFO @ Sun, 21 Jun 2020 20:15:50: 39000000 INFO @ Sun, 21 Jun 2020 20:15:53: 30000000 INFO @ Sun, 21 Jun 2020 20:15:56: 32000000 INFO @ Sun, 21 Jun 2020 20:15:56: 40000000 INFO @ Sun, 21 Jun 2020 20:15:59: 31000000 INFO @ Sun, 21 Jun 2020 20:16:03: 41000000 INFO @ Sun, 21 Jun 2020 20:16:04: 33000000 INFO @ Sun, 21 Jun 2020 20:16:06: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:16:10: 42000000 INFO @ Sun, 21 Jun 2020 20:16:11: 34000000 INFO @ Sun, 21 Jun 2020 20:16:13: 33000000 INFO @ Sun, 21 Jun 2020 20:16:17: 43000000 INFO @ Sun, 21 Jun 2020 20:16:19: 35000000 INFO @ Sun, 21 Jun 2020 20:16:20: 34000000 INFO @ Sun, 21 Jun 2020 20:16:23: 44000000 INFO @ Sun, 21 Jun 2020 20:16:26: 35000000 INFO @ Sun, 21 Jun 2020 20:16:27: 36000000 INFO @ Sun, 21 Jun 2020 20:16:30: 45000000 INFO @ Sun, 21 Jun 2020 20:16:33: 36000000 INFO @ Sun, 21 Jun 2020 20:16:34: 37000000 INFO @ Sun, 21 Jun 2020 20:16:37: 46000000 INFO @ Sun, 21 Jun 2020 20:16:40: 37000000 INFO @ Sun, 21 Jun 2020 20:16:42: 38000000 INFO @ Sun, 21 Jun 2020 20:16:43: 47000000 INFO @ Sun, 21 Jun 2020 20:16:46: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:16:46: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:16:46: #1 total tags in treatment: 47270944 INFO @ Sun, 21 Jun 2020 20:16:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:16:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:16:46: 38000000 INFO @ Sun, 21 Jun 2020 20:16:47: #1 tags after filtering in treatment: 47270930 INFO @ Sun, 21 Jun 2020 20:16:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:16:47: #1 finished! INFO @ Sun, 21 Jun 2020 20:16:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:16:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:16:49: 39000000 INFO @ Sun, 21 Jun 2020 20:16:50: #2 number of paired peaks: 57 WARNING @ Sun, 21 Jun 2020 20:16:50: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:16:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:16:53: 39000000 INFO @ Sun, 21 Jun 2020 20:16:57: 40000000 INFO @ Sun, 21 Jun 2020 20:16:59: 40000000 INFO @ Sun, 21 Jun 2020 20:17:04: 41000000 INFO @ Sun, 21 Jun 2020 20:17:06: 41000000 INFO @ Sun, 21 Jun 2020 20:17:12: 42000000 INFO @ Sun, 21 Jun 2020 20:17:13: 42000000 INFO @ Sun, 21 Jun 2020 20:17:19: 43000000 INFO @ Sun, 21 Jun 2020 20:17:20: 43000000 INFO @ Sun, 21 Jun 2020 20:17:28: 44000000 INFO @ Sun, 21 Jun 2020 20:17:28: 44000000 INFO @ Sun, 21 Jun 2020 20:17:36: 45000000 INFO @ Sun, 21 Jun 2020 20:17:36: 45000000 INFO @ Sun, 21 Jun 2020 20:17:43: 46000000 INFO @ Sun, 21 Jun 2020 20:17:43: 46000000 INFO @ Sun, 21 Jun 2020 20:17:50: 47000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:17:51: 47000000 INFO @ Sun, 21 Jun 2020 20:17:52: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:17:52: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:17:52: #1 total tags in treatment: 47270944 INFO @ Sun, 21 Jun 2020 20:17:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:53: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:17:53: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:17:53: #1 total tags in treatment: 47270944 INFO @ Sun, 21 Jun 2020 20:17:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:53: #1 tags after filtering in treatment: 47270930 INFO @ Sun, 21 Jun 2020 20:17:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:53: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:54: #1 tags after filtering in treatment: 47270930 INFO @ Sun, 21 Jun 2020 20:17:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:54: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:56: #2 number of paired peaks: 57 WARNING @ Sun, 21 Jun 2020 20:17:56: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:17:57: #2 number of paired peaks: 57 WARNING @ Sun, 21 Jun 2020 20:17:57: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166312/SRX3166312.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling