Job ID = 6456048 SRX = SRX3166310 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:29:36 prefetch.2.10.7: 1) Downloading 'SRR6012926'... 2020-06-21T10:29:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:34:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:34:59 prefetch.2.10.7: 1) 'SRR6012926' was downloaded successfully 2020-06-21T10:34:59 prefetch.2.10.7: 'SRR6012926' has 0 unresolved dependencies Read 59936919 spots for SRR6012926/SRR6012926.sra Written 59936919 spots for SRR6012926/SRR6012926.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:41 59936919 reads; of these: 59936919 (100.00%) were unpaired; of these: 2383656 (3.98%) aligned 0 times 47603472 (79.42%) aligned exactly 1 time 9949791 (16.60%) aligned >1 times 96.02% overall alignment rate Time searching: 00:14:41 Overall time: 00:14:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9388666 / 57553263 = 0.1631 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:19: 1000000 INFO @ Sun, 21 Jun 2020 20:03:24: 2000000 INFO @ Sun, 21 Jun 2020 20:03:29: 3000000 INFO @ Sun, 21 Jun 2020 20:03:34: 4000000 INFO @ Sun, 21 Jun 2020 20:03:40: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:45: 6000000 INFO @ Sun, 21 Jun 2020 20:03:50: 1000000 INFO @ Sun, 21 Jun 2020 20:03:50: 7000000 INFO @ Sun, 21 Jun 2020 20:03:55: 2000000 INFO @ Sun, 21 Jun 2020 20:03:55: 8000000 INFO @ Sun, 21 Jun 2020 20:04:00: 3000000 INFO @ Sun, 21 Jun 2020 20:04:01: 9000000 INFO @ Sun, 21 Jun 2020 20:04:06: 4000000 INFO @ Sun, 21 Jun 2020 20:04:06: 10000000 INFO @ Sun, 21 Jun 2020 20:04:11: 5000000 INFO @ Sun, 21 Jun 2020 20:04:12: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:17: 6000000 INFO @ Sun, 21 Jun 2020 20:04:17: 12000000 INFO @ Sun, 21 Jun 2020 20:04:21: 1000000 INFO @ Sun, 21 Jun 2020 20:04:23: 7000000 INFO @ Sun, 21 Jun 2020 20:04:23: 13000000 INFO @ Sun, 21 Jun 2020 20:04:27: 2000000 INFO @ Sun, 21 Jun 2020 20:04:29: 8000000 INFO @ Sun, 21 Jun 2020 20:04:29: 14000000 INFO @ Sun, 21 Jun 2020 20:04:33: 3000000 INFO @ Sun, 21 Jun 2020 20:04:35: 9000000 INFO @ Sun, 21 Jun 2020 20:04:35: 15000000 INFO @ Sun, 21 Jun 2020 20:04:40: 4000000 INFO @ Sun, 21 Jun 2020 20:04:41: 10000000 INFO @ Sun, 21 Jun 2020 20:04:41: 16000000 INFO @ Sun, 21 Jun 2020 20:04:46: 5000000 INFO @ Sun, 21 Jun 2020 20:04:46: 11000000 INFO @ Sun, 21 Jun 2020 20:04:47: 17000000 INFO @ Sun, 21 Jun 2020 20:04:52: 12000000 INFO @ Sun, 21 Jun 2020 20:04:53: 18000000 INFO @ Sun, 21 Jun 2020 20:04:53: 6000000 INFO @ Sun, 21 Jun 2020 20:04:58: 13000000 INFO @ Sun, 21 Jun 2020 20:04:58: 19000000 INFO @ Sun, 21 Jun 2020 20:04:59: 7000000 INFO @ Sun, 21 Jun 2020 20:05:04: 14000000 INFO @ Sun, 21 Jun 2020 20:05:04: 20000000 INFO @ Sun, 21 Jun 2020 20:05:06: 8000000 INFO @ Sun, 21 Jun 2020 20:05:10: 21000000 INFO @ Sun, 21 Jun 2020 20:05:10: 15000000 INFO @ Sun, 21 Jun 2020 20:05:12: 9000000 INFO @ Sun, 21 Jun 2020 20:05:16: 22000000 INFO @ Sun, 21 Jun 2020 20:05:16: 16000000 INFO @ Sun, 21 Jun 2020 20:05:18: 10000000 INFO @ Sun, 21 Jun 2020 20:05:22: 23000000 INFO @ Sun, 21 Jun 2020 20:05:22: 17000000 INFO @ Sun, 21 Jun 2020 20:05:25: 11000000 INFO @ Sun, 21 Jun 2020 20:05:28: 24000000 INFO @ Sun, 21 Jun 2020 20:05:28: 18000000 INFO @ Sun, 21 Jun 2020 20:05:31: 12000000 INFO @ Sun, 21 Jun 2020 20:05:34: 25000000 INFO @ Sun, 21 Jun 2020 20:05:34: 19000000 INFO @ Sun, 21 Jun 2020 20:05:37: 13000000 INFO @ Sun, 21 Jun 2020 20:05:39: 26000000 INFO @ Sun, 21 Jun 2020 20:05:40: 20000000 INFO @ Sun, 21 Jun 2020 20:05:43: 14000000 INFO @ Sun, 21 Jun 2020 20:05:45: 27000000 INFO @ Sun, 21 Jun 2020 20:05:46: 21000000 INFO @ Sun, 21 Jun 2020 20:05:49: 15000000 INFO @ Sun, 21 Jun 2020 20:05:51: 28000000 INFO @ Sun, 21 Jun 2020 20:05:51: 22000000 INFO @ Sun, 21 Jun 2020 20:05:56: 16000000 INFO @ Sun, 21 Jun 2020 20:05:57: 29000000 INFO @ Sun, 21 Jun 2020 20:05:57: 23000000 INFO @ Sun, 21 Jun 2020 20:06:02: 17000000 INFO @ Sun, 21 Jun 2020 20:06:03: 30000000 INFO @ Sun, 21 Jun 2020 20:06:03: 24000000 INFO @ Sun, 21 Jun 2020 20:06:08: 18000000 INFO @ Sun, 21 Jun 2020 20:06:08: 31000000 INFO @ Sun, 21 Jun 2020 20:06:09: 25000000 INFO @ Sun, 21 Jun 2020 20:06:14: 32000000 INFO @ Sun, 21 Jun 2020 20:06:15: 19000000 INFO @ Sun, 21 Jun 2020 20:06:15: 26000000 INFO @ Sun, 21 Jun 2020 20:06:20: 33000000 INFO @ Sun, 21 Jun 2020 20:06:20: 27000000 INFO @ Sun, 21 Jun 2020 20:06:21: 20000000 INFO @ Sun, 21 Jun 2020 20:06:26: 34000000 INFO @ Sun, 21 Jun 2020 20:06:26: 28000000 INFO @ Sun, 21 Jun 2020 20:06:27: 21000000 INFO @ Sun, 21 Jun 2020 20:06:32: 35000000 INFO @ Sun, 21 Jun 2020 20:06:32: 29000000 INFO @ Sun, 21 Jun 2020 20:06:33: 22000000 INFO @ Sun, 21 Jun 2020 20:06:37: 36000000 INFO @ Sun, 21 Jun 2020 20:06:38: 30000000 INFO @ Sun, 21 Jun 2020 20:06:39: 23000000 INFO @ Sun, 21 Jun 2020 20:06:43: 37000000 INFO @ Sun, 21 Jun 2020 20:06:43: 31000000 INFO @ Sun, 21 Jun 2020 20:06:45: 24000000 INFO @ Sun, 21 Jun 2020 20:06:49: 38000000 INFO @ Sun, 21 Jun 2020 20:06:49: 32000000 INFO @ Sun, 21 Jun 2020 20:06:52: 25000000 INFO @ Sun, 21 Jun 2020 20:06:55: 33000000 INFO @ Sun, 21 Jun 2020 20:06:55: 39000000 INFO @ Sun, 21 Jun 2020 20:06:58: 26000000 INFO @ Sun, 21 Jun 2020 20:07:00: 34000000 INFO @ Sun, 21 Jun 2020 20:07:01: 40000000 INFO @ Sun, 21 Jun 2020 20:07:04: 27000000 INFO @ Sun, 21 Jun 2020 20:07:06: 35000000 INFO @ Sun, 21 Jun 2020 20:07:07: 41000000 INFO @ Sun, 21 Jun 2020 20:07:11: 28000000 INFO @ Sun, 21 Jun 2020 20:07:12: 36000000 INFO @ Sun, 21 Jun 2020 20:07:13: 42000000 INFO @ Sun, 21 Jun 2020 20:07:17: 29000000 INFO @ Sun, 21 Jun 2020 20:07:18: 37000000 INFO @ Sun, 21 Jun 2020 20:07:18: 43000000 INFO @ Sun, 21 Jun 2020 20:07:23: 30000000 INFO @ Sun, 21 Jun 2020 20:07:24: 38000000 INFO @ Sun, 21 Jun 2020 20:07:24: 44000000 INFO @ Sun, 21 Jun 2020 20:07:29: 31000000 INFO @ Sun, 21 Jun 2020 20:07:30: 39000000 INFO @ Sun, 21 Jun 2020 20:07:30: 45000000 INFO @ Sun, 21 Jun 2020 20:07:35: 32000000 INFO @ Sun, 21 Jun 2020 20:07:36: 40000000 INFO @ Sun, 21 Jun 2020 20:07:36: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:07:41: 33000000 INFO @ Sun, 21 Jun 2020 20:07:42: 41000000 INFO @ Sun, 21 Jun 2020 20:07:42: 47000000 INFO @ Sun, 21 Jun 2020 20:07:47: 34000000 INFO @ Sun, 21 Jun 2020 20:07:48: 42000000 INFO @ Sun, 21 Jun 2020 20:07:48: 48000000 INFO @ Sun, 21 Jun 2020 20:07:49: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:07:49: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:07:49: #1 total tags in treatment: 48164597 INFO @ Sun, 21 Jun 2020 20:07:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:07:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:07:51: #1 tags after filtering in treatment: 48164566 INFO @ Sun, 21 Jun 2020 20:07:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:07:51: #1 finished! INFO @ Sun, 21 Jun 2020 20:07:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:07:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:07:54: 43000000 INFO @ Sun, 21 Jun 2020 20:07:54: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:07:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:07:54: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 20:07:54: 35000000 cut: /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:07:59: 44000000 INFO @ Sun, 21 Jun 2020 20:08:00: 36000000 INFO @ Sun, 21 Jun 2020 20:08:05: 45000000 INFO @ Sun, 21 Jun 2020 20:08:06: 37000000 INFO @ Sun, 21 Jun 2020 20:08:11: 46000000 INFO @ Sun, 21 Jun 2020 20:08:13: 38000000 INFO @ Sun, 21 Jun 2020 20:08:17: 47000000 INFO @ Sun, 21 Jun 2020 20:08:19: 39000000 INFO @ Sun, 21 Jun 2020 20:08:23: 48000000 INFO @ Sun, 21 Jun 2020 20:08:24: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:08:24: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:08:24: #1 total tags in treatment: 48164597 INFO @ Sun, 21 Jun 2020 20:08:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:08:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:08:25: #1 tags after filtering in treatment: 48164566 INFO @ Sun, 21 Jun 2020 20:08:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:08:25: #1 finished! INFO @ Sun, 21 Jun 2020 20:08:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:08:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:08:26: 40000000 INFO @ Sun, 21 Jun 2020 20:08:28: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:08:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:08:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:08:32: 41000000 INFO @ Sun, 21 Jun 2020 20:08:38: 42000000 INFO @ Sun, 21 Jun 2020 20:08:44: 43000000 INFO @ Sun, 21 Jun 2020 20:08:50: 44000000 INFO @ Sun, 21 Jun 2020 20:08:56: 45000000 INFO @ Sun, 21 Jun 2020 20:09:02: 46000000 INFO @ Sun, 21 Jun 2020 20:09:08: 47000000 INFO @ Sun, 21 Jun 2020 20:09:14: 48000000 INFO @ Sun, 21 Jun 2020 20:09:16: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:09:16: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:09:16: #1 total tags in treatment: 48164597 INFO @ Sun, 21 Jun 2020 20:09:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:09:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:09:17: #1 tags after filtering in treatment: 48164566 INFO @ Sun, 21 Jun 2020 20:09:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:09:17: #1 finished! INFO @ Sun, 21 Jun 2020 20:09:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:09:17: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:09:20: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:09:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:09:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3166310/SRX3166310.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling