Job ID = 6456045 SRX = SRX3159495 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:35:44 prefetch.2.10.7: 1) Downloading 'SRR6004225'... 2020-06-21T10:35:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:45:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:45:12 prefetch.2.10.7: 1) 'SRR6004225' was downloaded successfully 2020-06-21T10:45:12 prefetch.2.10.7: 'SRR6004225' has 0 unresolved dependencies Read 85937956 spots for SRR6004225/SRR6004225.sra Written 85937956 spots for SRR6004225/SRR6004225.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:12 85937956 reads; of these: 85937956 (100.00%) were unpaired; of these: 2591151 (3.02%) aligned 0 times 58467144 (68.03%) aligned exactly 1 time 24879661 (28.95%) aligned >1 times 96.98% overall alignment rate Time searching: 00:25:12 Overall time: 00:25:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 22566038 / 83346805 = 0.2707 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:28:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:28:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:28:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:28:52: 1000000 INFO @ Sun, 21 Jun 2020 20:28:59: 2000000 INFO @ Sun, 21 Jun 2020 20:29:05: 3000000 INFO @ Sun, 21 Jun 2020 20:29:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:19: 5000000 INFO @ Sun, 21 Jun 2020 20:29:23: 1000000 INFO @ Sun, 21 Jun 2020 20:29:26: 6000000 INFO @ Sun, 21 Jun 2020 20:29:29: 2000000 INFO @ Sun, 21 Jun 2020 20:29:34: 7000000 INFO @ Sun, 21 Jun 2020 20:29:36: 3000000 INFO @ Sun, 21 Jun 2020 20:29:41: 8000000 INFO @ Sun, 21 Jun 2020 20:29:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:48: 9000000 INFO @ Sun, 21 Jun 2020 20:29:50: 5000000 INFO @ Sun, 21 Jun 2020 20:29:53: 1000000 INFO @ Sun, 21 Jun 2020 20:29:56: 10000000 INFO @ Sun, 21 Jun 2020 20:29:57: 6000000 INFO @ Sun, 21 Jun 2020 20:30:00: 2000000 INFO @ Sun, 21 Jun 2020 20:30:04: 11000000 INFO @ Sun, 21 Jun 2020 20:30:04: 7000000 INFO @ Sun, 21 Jun 2020 20:30:07: 3000000 INFO @ Sun, 21 Jun 2020 20:30:11: 8000000 INFO @ Sun, 21 Jun 2020 20:30:11: 12000000 INFO @ Sun, 21 Jun 2020 20:30:14: 4000000 INFO @ Sun, 21 Jun 2020 20:30:18: 9000000 INFO @ Sun, 21 Jun 2020 20:30:19: 13000000 INFO @ Sun, 21 Jun 2020 20:30:21: 5000000 INFO @ Sun, 21 Jun 2020 20:30:25: 10000000 INFO @ Sun, 21 Jun 2020 20:30:27: 14000000 INFO @ Sun, 21 Jun 2020 20:30:28: 6000000 INFO @ Sun, 21 Jun 2020 20:30:32: 11000000 INFO @ Sun, 21 Jun 2020 20:30:34: 15000000 INFO @ Sun, 21 Jun 2020 20:30:35: 7000000 INFO @ Sun, 21 Jun 2020 20:30:39: 12000000 INFO @ Sun, 21 Jun 2020 20:30:42: 16000000 INFO @ Sun, 21 Jun 2020 20:30:42: 8000000 INFO @ Sun, 21 Jun 2020 20:30:46: 13000000 INFO @ Sun, 21 Jun 2020 20:30:49: 9000000 INFO @ Sun, 21 Jun 2020 20:30:50: 17000000 INFO @ Sun, 21 Jun 2020 20:30:53: 14000000 INFO @ Sun, 21 Jun 2020 20:30:56: 10000000 INFO @ Sun, 21 Jun 2020 20:30:57: 18000000 INFO @ Sun, 21 Jun 2020 20:31:00: 15000000 INFO @ Sun, 21 Jun 2020 20:31:03: 11000000 INFO @ Sun, 21 Jun 2020 20:31:05: 19000000 INFO @ Sun, 21 Jun 2020 20:31:07: 16000000 INFO @ Sun, 21 Jun 2020 20:31:10: 12000000 INFO @ Sun, 21 Jun 2020 20:31:13: 20000000 INFO @ Sun, 21 Jun 2020 20:31:14: 17000000 INFO @ Sun, 21 Jun 2020 20:31:18: 13000000 INFO @ Sun, 21 Jun 2020 20:31:21: 21000000 INFO @ Sun, 21 Jun 2020 20:31:21: 18000000 INFO @ Sun, 21 Jun 2020 20:31:24: 14000000 INFO @ Sun, 21 Jun 2020 20:31:28: 19000000 INFO @ Sun, 21 Jun 2020 20:31:28: 22000000 INFO @ Sun, 21 Jun 2020 20:31:31: 15000000 INFO @ Sun, 21 Jun 2020 20:31:35: 20000000 INFO @ Sun, 21 Jun 2020 20:31:36: 23000000 INFO @ Sun, 21 Jun 2020 20:31:38: 16000000 INFO @ Sun, 21 Jun 2020 20:31:42: 21000000 INFO @ Sun, 21 Jun 2020 20:31:44: 24000000 INFO @ Sun, 21 Jun 2020 20:31:45: 17000000 INFO @ Sun, 21 Jun 2020 20:31:49: 22000000 INFO @ Sun, 21 Jun 2020 20:31:52: 25000000 INFO @ Sun, 21 Jun 2020 20:31:52: 18000000 INFO @ Sun, 21 Jun 2020 20:31:56: 23000000 INFO @ Sun, 21 Jun 2020 20:31:59: 19000000 INFO @ Sun, 21 Jun 2020 20:32:00: 26000000 INFO @ Sun, 21 Jun 2020 20:32:03: 24000000 INFO @ Sun, 21 Jun 2020 20:32:06: 20000000 INFO @ Sun, 21 Jun 2020 20:32:08: 27000000 INFO @ Sun, 21 Jun 2020 20:32:10: 25000000 INFO @ Sun, 21 Jun 2020 20:32:13: 21000000 INFO @ Sun, 21 Jun 2020 20:32:15: 28000000 INFO @ Sun, 21 Jun 2020 20:32:17: 26000000 INFO @ Sun, 21 Jun 2020 20:32:20: 22000000 INFO @ Sun, 21 Jun 2020 20:32:23: 29000000 INFO @ Sun, 21 Jun 2020 20:32:24: 27000000 INFO @ Sun, 21 Jun 2020 20:32:27: 23000000 INFO @ Sun, 21 Jun 2020 20:32:31: 30000000 INFO @ Sun, 21 Jun 2020 20:32:31: 28000000 INFO @ Sun, 21 Jun 2020 20:32:34: 24000000 INFO @ Sun, 21 Jun 2020 20:32:38: 29000000 INFO @ Sun, 21 Jun 2020 20:32:38: 31000000 INFO @ Sun, 21 Jun 2020 20:32:41: 25000000 INFO @ Sun, 21 Jun 2020 20:32:45: 30000000 INFO @ Sun, 21 Jun 2020 20:32:46: 32000000 INFO @ Sun, 21 Jun 2020 20:32:48: 26000000 INFO @ Sun, 21 Jun 2020 20:32:52: 31000000 INFO @ Sun, 21 Jun 2020 20:32:53: 33000000 INFO @ Sun, 21 Jun 2020 20:32:55: 27000000 INFO @ Sun, 21 Jun 2020 20:32:59: 32000000 INFO @ Sun, 21 Jun 2020 20:33:01: 34000000 INFO @ Sun, 21 Jun 2020 20:33:02: 28000000 INFO @ Sun, 21 Jun 2020 20:33:06: 33000000 INFO @ Sun, 21 Jun 2020 20:33:09: 35000000 INFO @ Sun, 21 Jun 2020 20:33:09: 29000000 INFO @ Sun, 21 Jun 2020 20:33:13: 34000000 INFO @ Sun, 21 Jun 2020 20:33:16: 30000000 INFO @ Sun, 21 Jun 2020 20:33:16: 36000000 INFO @ Sun, 21 Jun 2020 20:33:20: 35000000 INFO @ Sun, 21 Jun 2020 20:33:23: 31000000 INFO @ Sun, 21 Jun 2020 20:33:24: 37000000 INFO @ Sun, 21 Jun 2020 20:33:27: 36000000 INFO @ Sun, 21 Jun 2020 20:33:30: 32000000 INFO @ Sun, 21 Jun 2020 20:33:32: 38000000 INFO @ Sun, 21 Jun 2020 20:33:34: 37000000 INFO @ Sun, 21 Jun 2020 20:33:38: 33000000 INFO @ Sun, 21 Jun 2020 20:33:39: 39000000 INFO @ Sun, 21 Jun 2020 20:33:42: 38000000 INFO @ Sun, 21 Jun 2020 20:33:45: 34000000 INFO @ Sun, 21 Jun 2020 20:33:47: 40000000 INFO @ Sun, 21 Jun 2020 20:33:49: 39000000 INFO @ Sun, 21 Jun 2020 20:33:52: 35000000 INFO @ Sun, 21 Jun 2020 20:33:55: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:33:56: 40000000 INFO @ Sun, 21 Jun 2020 20:33:59: 36000000 INFO @ Sun, 21 Jun 2020 20:34:02: 42000000 INFO @ Sun, 21 Jun 2020 20:34:03: 41000000 INFO @ Sun, 21 Jun 2020 20:34:06: 37000000 INFO @ Sun, 21 Jun 2020 20:34:10: 43000000 INFO @ Sun, 21 Jun 2020 20:34:10: 42000000 INFO @ Sun, 21 Jun 2020 20:34:13: 38000000 INFO @ Sun, 21 Jun 2020 20:34:17: 43000000 INFO @ Sun, 21 Jun 2020 20:34:18: 44000000 INFO @ Sun, 21 Jun 2020 20:34:21: 39000000 INFO @ Sun, 21 Jun 2020 20:34:24: 44000000 INFO @ Sun, 21 Jun 2020 20:34:25: 45000000 INFO @ Sun, 21 Jun 2020 20:34:28: 40000000 INFO @ Sun, 21 Jun 2020 20:34:32: 45000000 INFO @ Sun, 21 Jun 2020 20:34:33: 46000000 INFO @ Sun, 21 Jun 2020 20:34:35: 41000000 INFO @ Sun, 21 Jun 2020 20:34:39: 46000000 INFO @ Sun, 21 Jun 2020 20:34:40: 47000000 INFO @ Sun, 21 Jun 2020 20:34:42: 42000000 INFO @ Sun, 21 Jun 2020 20:34:46: 47000000 INFO @ Sun, 21 Jun 2020 20:34:48: 48000000 INFO @ Sun, 21 Jun 2020 20:34:49: 43000000 INFO @ Sun, 21 Jun 2020 20:34:53: 48000000 INFO @ Sun, 21 Jun 2020 20:34:56: 49000000 INFO @ Sun, 21 Jun 2020 20:34:56: 44000000 INFO @ Sun, 21 Jun 2020 20:35:00: 49000000 INFO @ Sun, 21 Jun 2020 20:35:03: 45000000 INFO @ Sun, 21 Jun 2020 20:35:03: 50000000 INFO @ Sun, 21 Jun 2020 20:35:07: 50000000 INFO @ Sun, 21 Jun 2020 20:35:10: 46000000 INFO @ Sun, 21 Jun 2020 20:35:11: 51000000 INFO @ Sun, 21 Jun 2020 20:35:14: 51000000 INFO @ Sun, 21 Jun 2020 20:35:17: 47000000 INFO @ Sun, 21 Jun 2020 20:35:18: 52000000 INFO @ Sun, 21 Jun 2020 20:35:21: 52000000 INFO @ Sun, 21 Jun 2020 20:35:24: 48000000 INFO @ Sun, 21 Jun 2020 20:35:26: 53000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:35:28: 53000000 INFO @ Sun, 21 Jun 2020 20:35:31: 49000000 INFO @ Sun, 21 Jun 2020 20:35:33: 54000000 INFO @ Sun, 21 Jun 2020 20:35:35: 54000000 INFO @ Sun, 21 Jun 2020 20:35:38: 50000000 INFO @ Sun, 21 Jun 2020 20:35:41: 55000000 INFO @ Sun, 21 Jun 2020 20:35:42: 55000000 INFO @ Sun, 21 Jun 2020 20:35:45: 51000000 INFO @ Sun, 21 Jun 2020 20:35:49: 56000000 INFO @ Sun, 21 Jun 2020 20:35:50: 56000000 INFO @ Sun, 21 Jun 2020 20:35:52: 52000000 INFO @ Sun, 21 Jun 2020 20:35:57: 57000000 INFO @ Sun, 21 Jun 2020 20:35:58: 57000000 INFO @ Sun, 21 Jun 2020 20:35:59: 53000000 INFO @ Sun, 21 Jun 2020 20:36:05: 58000000 INFO @ Sun, 21 Jun 2020 20:36:06: 58000000 INFO @ Sun, 21 Jun 2020 20:36:07: 54000000 INFO @ Sun, 21 Jun 2020 20:36:14: 59000000 INFO @ Sun, 21 Jun 2020 20:36:14: 55000000 INFO @ Sun, 21 Jun 2020 20:36:14: 59000000 INFO @ Sun, 21 Jun 2020 20:36:21: 56000000 INFO @ Sun, 21 Jun 2020 20:36:22: 60000000 INFO @ Sun, 21 Jun 2020 20:36:23: 60000000 INFO @ Sun, 21 Jun 2020 20:36:28: 57000000 INFO @ Sun, 21 Jun 2020 20:36:29: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:36:29: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:36:29: #1 total tags in treatment: 60780767 INFO @ Sun, 21 Jun 2020 20:36:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:36:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:36:29: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:36:29: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:36:29: #1 total tags in treatment: 60780767 INFO @ Sun, 21 Jun 2020 20:36:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:36:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:36:30: #1 tags after filtering in treatment: 60780767 INFO @ Sun, 21 Jun 2020 20:36:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:36:30: #1 finished! INFO @ Sun, 21 Jun 2020 20:36:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:36:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:36:31: #1 tags after filtering in treatment: 60780767 INFO @ Sun, 21 Jun 2020 20:36:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:36:31: #1 finished! INFO @ Sun, 21 Jun 2020 20:36:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:36:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:36:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:36:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:36:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:36:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:36:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:36:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:36:35: 58000000 INFO @ Sun, 21 Jun 2020 20:36:41: 59000000 INFO @ Sun, 21 Jun 2020 20:36:47: 60000000 INFO @ Sun, 21 Jun 2020 20:36:53: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:36:53: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:36:53: #1 total tags in treatment: 60780767 INFO @ Sun, 21 Jun 2020 20:36:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:36:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:36:54: #1 tags after filtering in treatment: 60780767 INFO @ Sun, 21 Jun 2020 20:36:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:36:54: #1 finished! INFO @ Sun, 21 Jun 2020 20:36:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:36:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:36:57: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:36:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:36:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159495/SRX3159495.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling