Job ID = 6456013 SRX = SRX3159470 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:45:53 prefetch.2.10.7: 1) Downloading 'SRR6004200'... 2020-06-21T10:45:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:51:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:51:44 prefetch.2.10.7: 1) 'SRR6004200' was downloaded successfully 2020-06-21T10:51:44 prefetch.2.10.7: 'SRR6004200' has 0 unresolved dependencies Read 69945224 spots for SRR6004200/SRR6004200.sra Written 69945224 spots for SRR6004200/SRR6004200.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:57 69945224 reads; of these: 69945224 (100.00%) were unpaired; of these: 2258048 (3.23%) aligned 0 times 55264792 (79.01%) aligned exactly 1 time 12422384 (17.76%) aligned >1 times 96.77% overall alignment rate Time searching: 00:18:57 Overall time: 00:18:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 29474071 / 67687176 = 0.4354 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:24:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:24:56: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:24:56: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:25:01: 1000000 INFO @ Sun, 21 Jun 2020 20:25:05: 2000000 INFO @ Sun, 21 Jun 2020 20:25:10: 3000000 INFO @ Sun, 21 Jun 2020 20:25:15: 4000000 INFO @ Sun, 21 Jun 2020 20:25:19: 5000000 INFO @ Sun, 21 Jun 2020 20:25:24: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:25:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:25:26: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:25:26: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:25:28: 7000000 INFO @ Sun, 21 Jun 2020 20:25:31: 1000000 INFO @ Sun, 21 Jun 2020 20:25:33: 8000000 INFO @ Sun, 21 Jun 2020 20:25:36: 2000000 INFO @ Sun, 21 Jun 2020 20:25:38: 9000000 INFO @ Sun, 21 Jun 2020 20:25:40: 3000000 INFO @ Sun, 21 Jun 2020 20:25:43: 10000000 INFO @ Sun, 21 Jun 2020 20:25:45: 4000000 INFO @ Sun, 21 Jun 2020 20:25:48: 11000000 INFO @ Sun, 21 Jun 2020 20:25:50: 5000000 INFO @ Sun, 21 Jun 2020 20:25:52: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:25:55: 6000000 INFO @ Sun, 21 Jun 2020 20:25:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:25:56: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:25:56: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:25:57: 13000000 INFO @ Sun, 21 Jun 2020 20:25:59: 7000000 INFO @ Sun, 21 Jun 2020 20:26:01: 1000000 INFO @ Sun, 21 Jun 2020 20:26:02: 14000000 INFO @ Sun, 21 Jun 2020 20:26:04: 8000000 INFO @ Sun, 21 Jun 2020 20:26:06: 2000000 INFO @ Sun, 21 Jun 2020 20:26:06: 15000000 INFO @ Sun, 21 Jun 2020 20:26:09: 9000000 INFO @ Sun, 21 Jun 2020 20:26:10: 3000000 INFO @ Sun, 21 Jun 2020 20:26:11: 16000000 INFO @ Sun, 21 Jun 2020 20:26:13: 10000000 INFO @ Sun, 21 Jun 2020 20:26:15: 4000000 INFO @ Sun, 21 Jun 2020 20:26:16: 17000000 INFO @ Sun, 21 Jun 2020 20:26:18: 11000000 INFO @ Sun, 21 Jun 2020 20:26:20: 5000000 INFO @ Sun, 21 Jun 2020 20:26:20: 18000000 INFO @ Sun, 21 Jun 2020 20:26:23: 12000000 INFO @ Sun, 21 Jun 2020 20:26:25: 6000000 INFO @ Sun, 21 Jun 2020 20:26:25: 19000000 INFO @ Sun, 21 Jun 2020 20:26:28: 13000000 INFO @ Sun, 21 Jun 2020 20:26:29: 7000000 INFO @ Sun, 21 Jun 2020 20:26:30: 20000000 INFO @ Sun, 21 Jun 2020 20:26:32: 14000000 INFO @ Sun, 21 Jun 2020 20:26:34: 8000000 INFO @ Sun, 21 Jun 2020 20:26:34: 21000000 INFO @ Sun, 21 Jun 2020 20:26:37: 15000000 INFO @ Sun, 21 Jun 2020 20:26:39: 9000000 INFO @ Sun, 21 Jun 2020 20:26:39: 22000000 INFO @ Sun, 21 Jun 2020 20:26:41: 16000000 INFO @ Sun, 21 Jun 2020 20:26:44: 10000000 INFO @ Sun, 21 Jun 2020 20:26:44: 23000000 INFO @ Sun, 21 Jun 2020 20:26:46: 17000000 INFO @ Sun, 21 Jun 2020 20:26:48: 11000000 INFO @ Sun, 21 Jun 2020 20:26:49: 24000000 INFO @ Sun, 21 Jun 2020 20:26:51: 18000000 INFO @ Sun, 21 Jun 2020 20:26:53: 12000000 INFO @ Sun, 21 Jun 2020 20:26:53: 25000000 INFO @ Sun, 21 Jun 2020 20:26:55: 19000000 INFO @ Sun, 21 Jun 2020 20:26:58: 13000000 INFO @ Sun, 21 Jun 2020 20:26:58: 26000000 INFO @ Sun, 21 Jun 2020 20:27:00: 20000000 INFO @ Sun, 21 Jun 2020 20:27:02: 14000000 INFO @ Sun, 21 Jun 2020 20:27:03: 27000000 INFO @ Sun, 21 Jun 2020 20:27:05: 21000000 INFO @ Sun, 21 Jun 2020 20:27:07: 15000000 INFO @ Sun, 21 Jun 2020 20:27:07: 28000000 INFO @ Sun, 21 Jun 2020 20:27:10: 22000000 INFO @ Sun, 21 Jun 2020 20:27:12: 16000000 INFO @ Sun, 21 Jun 2020 20:27:12: 29000000 INFO @ Sun, 21 Jun 2020 20:27:14: 23000000 INFO @ Sun, 21 Jun 2020 20:27:16: 17000000 INFO @ Sun, 21 Jun 2020 20:27:17: 30000000 INFO @ Sun, 21 Jun 2020 20:27:19: 24000000 INFO @ Sun, 21 Jun 2020 20:27:21: 18000000 INFO @ Sun, 21 Jun 2020 20:27:21: 31000000 INFO @ Sun, 21 Jun 2020 20:27:24: 25000000 INFO @ Sun, 21 Jun 2020 20:27:25: 19000000 INFO @ Sun, 21 Jun 2020 20:27:26: 32000000 INFO @ Sun, 21 Jun 2020 20:27:28: 26000000 INFO @ Sun, 21 Jun 2020 20:27:30: 20000000 INFO @ Sun, 21 Jun 2020 20:27:31: 33000000 INFO @ Sun, 21 Jun 2020 20:27:33: 27000000 INFO @ Sun, 21 Jun 2020 20:27:35: 21000000 INFO @ Sun, 21 Jun 2020 20:27:36: 34000000 INFO @ Sun, 21 Jun 2020 20:27:38: 28000000 INFO @ Sun, 21 Jun 2020 20:27:39: 22000000 INFO @ Sun, 21 Jun 2020 20:27:40: 35000000 INFO @ Sun, 21 Jun 2020 20:27:42: 29000000 INFO @ Sun, 21 Jun 2020 20:27:44: 23000000 INFO @ Sun, 21 Jun 2020 20:27:45: 36000000 INFO @ Sun, 21 Jun 2020 20:27:47: 30000000 INFO @ Sun, 21 Jun 2020 20:27:49: 24000000 INFO @ Sun, 21 Jun 2020 20:27:50: 37000000 INFO @ Sun, 21 Jun 2020 20:27:52: 31000000 INFO @ Sun, 21 Jun 2020 20:27:53: 25000000 INFO @ Sun, 21 Jun 2020 20:27:55: 38000000 INFO @ Sun, 21 Jun 2020 20:27:56: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:27:56: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:27:56: #1 total tags in treatment: 38213105 INFO @ Sun, 21 Jun 2020 20:27:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:27:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:27:57: 32000000 INFO @ Sun, 21 Jun 2020 20:27:57: #1 tags after filtering in treatment: 38213103 INFO @ Sun, 21 Jun 2020 20:27:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:27:57: #1 finished! INFO @ Sun, 21 Jun 2020 20:27:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:27:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:27:58: 26000000 INFO @ Sun, 21 Jun 2020 20:27:59: #2 number of paired peaks: 46 WARNING @ Sun, 21 Jun 2020 20:27:59: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:27:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:28:01: 33000000 INFO @ Sun, 21 Jun 2020 20:28:03: 27000000 INFO @ Sun, 21 Jun 2020 20:28:06: 34000000 INFO @ Sun, 21 Jun 2020 20:28:07: 28000000 INFO @ Sun, 21 Jun 2020 20:28:11: 35000000 INFO @ Sun, 21 Jun 2020 20:28:12: 29000000 INFO @ Sun, 21 Jun 2020 20:28:15: 36000000 INFO @ Sun, 21 Jun 2020 20:28:17: 30000000 INFO @ Sun, 21 Jun 2020 20:28:20: 37000000 INFO @ Sun, 21 Jun 2020 20:28:21: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:28:25: 38000000 INFO @ Sun, 21 Jun 2020 20:28:26: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:28:26: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:28:26: #1 total tags in treatment: 38213105 INFO @ Sun, 21 Jun 2020 20:28:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:28:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:28:26: 32000000 INFO @ Sun, 21 Jun 2020 20:28:27: #1 tags after filtering in treatment: 38213103 INFO @ Sun, 21 Jun 2020 20:28:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:28:27: #1 finished! INFO @ Sun, 21 Jun 2020 20:28:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:28:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:28:29: #2 number of paired peaks: 46 WARNING @ Sun, 21 Jun 2020 20:28:29: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:28:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:28:31: 33000000 INFO @ Sun, 21 Jun 2020 20:28:36: 34000000 INFO @ Sun, 21 Jun 2020 20:28:40: 35000000 INFO @ Sun, 21 Jun 2020 20:28:45: 36000000 INFO @ Sun, 21 Jun 2020 20:28:49: 37000000 INFO @ Sun, 21 Jun 2020 20:28:54: 38000000 INFO @ Sun, 21 Jun 2020 20:28:55: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:28:55: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:28:55: #1 total tags in treatment: 38213105 INFO @ Sun, 21 Jun 2020 20:28:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:28:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:28:56: #1 tags after filtering in treatment: 38213103 INFO @ Sun, 21 Jun 2020 20:28:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:28:56: #1 finished! INFO @ Sun, 21 Jun 2020 20:28:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:28:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:28:58: #2 number of paired peaks: 46 WARNING @ Sun, 21 Jun 2020 20:28:58: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:28:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159470/SRX3159470.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。