Job ID = 6456011 SRX = SRX3159468 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:23:21 prefetch.2.10.7: 1) Downloading 'SRR6004198'... 2020-06-21T10:23:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:29:40 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:29:40 prefetch.2.10.7: 1) 'SRR6004198' was downloaded successfully 2020-06-21T10:29:40 prefetch.2.10.7: 'SRR6004198' has 0 unresolved dependencies Read 65446102 spots for SRR6004198/SRR6004198.sra Written 65446102 spots for SRR6004198/SRR6004198.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:00 65446102 reads; of these: 65446102 (100.00%) were unpaired; of these: 1680510 (2.57%) aligned 0 times 45125848 (68.95%) aligned exactly 1 time 18639744 (28.48%) aligned >1 times 97.43% overall alignment rate Time searching: 00:19:00 Overall time: 00:19:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 15200206 / 63765592 = 0.2384 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:08: 1000000 INFO @ Sun, 21 Jun 2020 20:03:15: 2000000 INFO @ Sun, 21 Jun 2020 20:03:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:03:30: 4000000 INFO @ Sun, 21 Jun 2020 20:03:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:03:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:03:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:03:38: 5000000 INFO @ Sun, 21 Jun 2020 20:03:38: 1000000 INFO @ Sun, 21 Jun 2020 20:03:45: 2000000 INFO @ Sun, 21 Jun 2020 20:03:46: 6000000 INFO @ Sun, 21 Jun 2020 20:03:53: 3000000 INFO @ Sun, 21 Jun 2020 20:03:54: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:04:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:04:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:04:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:04:00: 4000000 INFO @ Sun, 21 Jun 2020 20:04:02: 8000000 INFO @ Sun, 21 Jun 2020 20:04:08: 5000000 INFO @ Sun, 21 Jun 2020 20:04:09: 1000000 INFO @ Sun, 21 Jun 2020 20:04:11: 9000000 INFO @ Sun, 21 Jun 2020 20:04:16: 6000000 INFO @ Sun, 21 Jun 2020 20:04:17: 2000000 INFO @ Sun, 21 Jun 2020 20:04:19: 10000000 INFO @ Sun, 21 Jun 2020 20:04:24: 7000000 INFO @ Sun, 21 Jun 2020 20:04:26: 3000000 INFO @ Sun, 21 Jun 2020 20:04:27: 11000000 INFO @ Sun, 21 Jun 2020 20:04:32: 8000000 INFO @ Sun, 21 Jun 2020 20:04:34: 4000000 INFO @ Sun, 21 Jun 2020 20:04:35: 12000000 INFO @ Sun, 21 Jun 2020 20:04:39: 9000000 INFO @ Sun, 21 Jun 2020 20:04:42: 5000000 INFO @ Sun, 21 Jun 2020 20:04:43: 13000000 INFO @ Sun, 21 Jun 2020 20:04:47: 10000000 INFO @ Sun, 21 Jun 2020 20:04:50: 6000000 INFO @ Sun, 21 Jun 2020 20:04:52: 14000000 INFO @ Sun, 21 Jun 2020 20:04:55: 11000000 INFO @ Sun, 21 Jun 2020 20:04:58: 7000000 INFO @ Sun, 21 Jun 2020 20:05:00: 15000000 INFO @ Sun, 21 Jun 2020 20:05:03: 12000000 INFO @ Sun, 21 Jun 2020 20:05:06: 8000000 INFO @ Sun, 21 Jun 2020 20:05:08: 16000000 INFO @ Sun, 21 Jun 2020 20:05:10: 13000000 INFO @ Sun, 21 Jun 2020 20:05:14: 9000000 INFO @ Sun, 21 Jun 2020 20:05:15: 17000000 INFO @ Sun, 21 Jun 2020 20:05:18: 14000000 INFO @ Sun, 21 Jun 2020 20:05:23: 10000000 INFO @ Sun, 21 Jun 2020 20:05:23: 18000000 INFO @ Sun, 21 Jun 2020 20:05:25: 15000000 INFO @ Sun, 21 Jun 2020 20:05:31: 11000000 INFO @ Sun, 21 Jun 2020 20:05:31: 19000000 INFO @ Sun, 21 Jun 2020 20:05:33: 16000000 INFO @ Sun, 21 Jun 2020 20:05:39: 20000000 INFO @ Sun, 21 Jun 2020 20:05:39: 12000000 INFO @ Sun, 21 Jun 2020 20:05:41: 17000000 INFO @ Sun, 21 Jun 2020 20:05:47: 21000000 INFO @ Sun, 21 Jun 2020 20:05:47: 13000000 INFO @ Sun, 21 Jun 2020 20:05:49: 18000000 INFO @ Sun, 21 Jun 2020 20:05:55: 22000000 INFO @ Sun, 21 Jun 2020 20:05:56: 14000000 INFO @ Sun, 21 Jun 2020 20:05:57: 19000000 INFO @ Sun, 21 Jun 2020 20:06:03: 23000000 INFO @ Sun, 21 Jun 2020 20:06:04: 15000000 INFO @ Sun, 21 Jun 2020 20:06:04: 20000000 INFO @ Sun, 21 Jun 2020 20:06:12: 24000000 INFO @ Sun, 21 Jun 2020 20:06:12: 21000000 INFO @ Sun, 21 Jun 2020 20:06:13: 16000000 INFO @ Sun, 21 Jun 2020 20:06:20: 25000000 INFO @ Sun, 21 Jun 2020 20:06:20: 22000000 INFO @ Sun, 21 Jun 2020 20:06:21: 17000000 INFO @ Sun, 21 Jun 2020 20:06:28: 23000000 INFO @ Sun, 21 Jun 2020 20:06:28: 26000000 INFO @ Sun, 21 Jun 2020 20:06:30: 18000000 INFO @ Sun, 21 Jun 2020 20:06:36: 24000000 INFO @ Sun, 21 Jun 2020 20:06:36: 27000000 INFO @ Sun, 21 Jun 2020 20:06:39: 19000000 INFO @ Sun, 21 Jun 2020 20:06:43: 25000000 INFO @ Sun, 21 Jun 2020 20:06:45: 28000000 INFO @ Sun, 21 Jun 2020 20:06:47: 20000000 INFO @ Sun, 21 Jun 2020 20:06:51: 26000000 INFO @ Sun, 21 Jun 2020 20:06:53: 29000000 INFO @ Sun, 21 Jun 2020 20:06:55: 21000000 INFO @ Sun, 21 Jun 2020 20:06:59: 27000000 INFO @ Sun, 21 Jun 2020 20:07:02: 30000000 INFO @ Sun, 21 Jun 2020 20:07:04: 22000000 INFO @ Sun, 21 Jun 2020 20:07:07: 28000000 INFO @ Sun, 21 Jun 2020 20:07:10: 31000000 INFO @ Sun, 21 Jun 2020 20:07:12: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:07:14: 29000000 INFO @ Sun, 21 Jun 2020 20:07:18: 32000000 INFO @ Sun, 21 Jun 2020 20:07:20: 24000000 INFO @ Sun, 21 Jun 2020 20:07:22: 30000000 INFO @ Sun, 21 Jun 2020 20:07:26: 33000000 INFO @ Sun, 21 Jun 2020 20:07:28: 25000000 INFO @ Sun, 21 Jun 2020 20:07:30: 31000000 INFO @ Sun, 21 Jun 2020 20:07:35: 34000000 INFO @ Sun, 21 Jun 2020 20:07:36: 26000000 INFO @ Sun, 21 Jun 2020 20:07:37: 32000000 INFO @ Sun, 21 Jun 2020 20:07:43: 35000000 INFO @ Sun, 21 Jun 2020 20:07:44: 27000000 INFO @ Sun, 21 Jun 2020 20:07:45: 33000000 INFO @ Sun, 21 Jun 2020 20:07:50: 36000000 INFO @ Sun, 21 Jun 2020 20:07:52: 34000000 INFO @ Sun, 21 Jun 2020 20:07:53: 28000000 INFO @ Sun, 21 Jun 2020 20:07:58: 37000000 INFO @ Sun, 21 Jun 2020 20:08:00: 35000000 INFO @ Sun, 21 Jun 2020 20:08:01: 29000000 INFO @ Sun, 21 Jun 2020 20:08:06: 38000000 INFO @ Sun, 21 Jun 2020 20:08:08: 36000000 INFO @ Sun, 21 Jun 2020 20:08:09: 30000000 INFO @ Sun, 21 Jun 2020 20:08:14: 39000000 INFO @ Sun, 21 Jun 2020 20:08:15: 37000000 INFO @ Sun, 21 Jun 2020 20:08:17: 31000000 INFO @ Sun, 21 Jun 2020 20:08:23: 40000000 INFO @ Sun, 21 Jun 2020 20:08:23: 38000000 INFO @ Sun, 21 Jun 2020 20:08:24: 32000000 INFO @ Sun, 21 Jun 2020 20:08:30: 41000000 INFO @ Sun, 21 Jun 2020 20:08:31: 39000000 INFO @ Sun, 21 Jun 2020 20:08:32: 33000000 INFO @ Sun, 21 Jun 2020 20:08:38: 42000000 INFO @ Sun, 21 Jun 2020 20:08:39: 40000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:08:40: 34000000 INFO @ Sun, 21 Jun 2020 20:08:46: 41000000 INFO @ Sun, 21 Jun 2020 20:08:46: 43000000 INFO @ Sun, 21 Jun 2020 20:08:48: 35000000 INFO @ Sun, 21 Jun 2020 20:08:54: 42000000 INFO @ Sun, 21 Jun 2020 20:08:54: 44000000 INFO @ Sun, 21 Jun 2020 20:08:57: 36000000 INFO @ Sun, 21 Jun 2020 20:09:01: 43000000 INFO @ Sun, 21 Jun 2020 20:09:02: 45000000 INFO @ Sun, 21 Jun 2020 20:09:05: 37000000 INFO @ Sun, 21 Jun 2020 20:09:09: 44000000 INFO @ Sun, 21 Jun 2020 20:09:10: 46000000 INFO @ Sun, 21 Jun 2020 20:09:12: 38000000 INFO @ Sun, 21 Jun 2020 20:09:16: 45000000 INFO @ Sun, 21 Jun 2020 20:09:18: 47000000 INFO @ Sun, 21 Jun 2020 20:09:21: 39000000 INFO @ Sun, 21 Jun 2020 20:09:23: 46000000 INFO @ Sun, 21 Jun 2020 20:09:26: 48000000 INFO @ Sun, 21 Jun 2020 20:09:29: 40000000 INFO @ Sun, 21 Jun 2020 20:09:31: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:09:31: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:09:31: #1 total tags in treatment: 48565386 INFO @ Sun, 21 Jun 2020 20:09:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:09:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:09:31: 47000000 INFO @ Sun, 21 Jun 2020 20:09:32: #1 tags after filtering in treatment: 48565386 INFO @ Sun, 21 Jun 2020 20:09:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:09:32: #1 finished! INFO @ Sun, 21 Jun 2020 20:09:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:09:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:09:35: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:09:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:09:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:09:37: 41000000 INFO @ Sun, 21 Jun 2020 20:09:39: 48000000 INFO @ Sun, 21 Jun 2020 20:09:43: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:09:43: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:09:43: #1 total tags in treatment: 48565386 INFO @ Sun, 21 Jun 2020 20:09:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:09:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:09:44: #1 tags after filtering in treatment: 48565386 INFO @ Sun, 21 Jun 2020 20:09:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:09:44: #1 finished! INFO @ Sun, 21 Jun 2020 20:09:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:09:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:09:45: 42000000 INFO @ Sun, 21 Jun 2020 20:09:47: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:09:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:09:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:09:52: 43000000 INFO @ Sun, 21 Jun 2020 20:09:59: 44000000 INFO @ Sun, 21 Jun 2020 20:10:06: 45000000 INFO @ Sun, 21 Jun 2020 20:10:13: 46000000 INFO @ Sun, 21 Jun 2020 20:10:20: 47000000 INFO @ Sun, 21 Jun 2020 20:10:27: 48000000 INFO @ Sun, 21 Jun 2020 20:10:32: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:10:32: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:10:32: #1 total tags in treatment: 48565386 INFO @ Sun, 21 Jun 2020 20:10:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:10:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:10:33: #1 tags after filtering in treatment: 48565386 INFO @ Sun, 21 Jun 2020 20:10:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:10:33: #1 finished! INFO @ Sun, 21 Jun 2020 20:10:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:10:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:10:36: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:10:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:10:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159468/SRX3159468.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling