Job ID = 6456007 SRX = SRX3159467 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:33:04 prefetch.2.10.7: 1) Downloading 'SRR6004197'... 2020-06-21T10:33:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:37:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:37:33 prefetch.2.10.7: 1) 'SRR6004197' was downloaded successfully 2020-06-21T10:37:33 prefetch.2.10.7: 'SRR6004197' has 0 unresolved dependencies Read 51859093 spots for SRR6004197/SRR6004197.sra Written 51859093 spots for SRR6004197/SRR6004197.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:02 51859093 reads; of these: 51859093 (100.00%) were unpaired; of these: 1475947 (2.85%) aligned 0 times 34576490 (66.67%) aligned exactly 1 time 15806656 (30.48%) aligned >1 times 97.15% overall alignment rate Time searching: 00:17:03 Overall time: 00:17:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10863562 / 50383146 = 0.2156 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:06:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:06:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:06:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:07:03: 1000000 INFO @ Sun, 21 Jun 2020 20:07:07: 2000000 INFO @ Sun, 21 Jun 2020 20:07:12: 3000000 INFO @ Sun, 21 Jun 2020 20:07:17: 4000000 INFO @ Sun, 21 Jun 2020 20:07:21: 5000000 INFO @ Sun, 21 Jun 2020 20:07:26: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:07:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:07:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:07:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:07:31: 7000000 INFO @ Sun, 21 Jun 2020 20:07:34: 1000000 INFO @ Sun, 21 Jun 2020 20:07:36: 8000000 INFO @ Sun, 21 Jun 2020 20:07:39: 2000000 INFO @ Sun, 21 Jun 2020 20:07:41: 9000000 INFO @ Sun, 21 Jun 2020 20:07:45: 3000000 INFO @ Sun, 21 Jun 2020 20:07:46: 10000000 INFO @ Sun, 21 Jun 2020 20:07:50: 4000000 INFO @ Sun, 21 Jun 2020 20:07:50: 11000000 INFO @ Sun, 21 Jun 2020 20:07:55: 12000000 INFO @ Sun, 21 Jun 2020 20:07:56: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:07:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:07:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:07:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:08:00: 13000000 INFO @ Sun, 21 Jun 2020 20:08:01: 6000000 INFO @ Sun, 21 Jun 2020 20:08:04: 1000000 INFO @ Sun, 21 Jun 2020 20:08:05: 14000000 INFO @ Sun, 21 Jun 2020 20:08:07: 7000000 INFO @ Sun, 21 Jun 2020 20:08:09: 2000000 INFO @ Sun, 21 Jun 2020 20:08:10: 15000000 INFO @ Sun, 21 Jun 2020 20:08:13: 8000000 INFO @ Sun, 21 Jun 2020 20:08:15: 16000000 INFO @ Sun, 21 Jun 2020 20:08:15: 3000000 INFO @ Sun, 21 Jun 2020 20:08:18: 9000000 INFO @ Sun, 21 Jun 2020 20:08:20: 17000000 INFO @ Sun, 21 Jun 2020 20:08:21: 4000000 INFO @ Sun, 21 Jun 2020 20:08:24: 10000000 INFO @ Sun, 21 Jun 2020 20:08:25: 18000000 INFO @ Sun, 21 Jun 2020 20:08:27: 5000000 INFO @ Sun, 21 Jun 2020 20:08:30: 19000000 INFO @ Sun, 21 Jun 2020 20:08:30: 11000000 INFO @ Sun, 21 Jun 2020 20:08:33: 6000000 INFO @ Sun, 21 Jun 2020 20:08:34: 20000000 INFO @ Sun, 21 Jun 2020 20:08:36: 12000000 INFO @ Sun, 21 Jun 2020 20:08:38: 7000000 INFO @ Sun, 21 Jun 2020 20:08:39: 21000000 INFO @ Sun, 21 Jun 2020 20:08:41: 13000000 INFO @ Sun, 21 Jun 2020 20:08:44: 22000000 INFO @ Sun, 21 Jun 2020 20:08:44: 8000000 INFO @ Sun, 21 Jun 2020 20:08:47: 14000000 INFO @ Sun, 21 Jun 2020 20:08:49: 23000000 INFO @ Sun, 21 Jun 2020 20:08:50: 9000000 INFO @ Sun, 21 Jun 2020 20:08:53: 15000000 INFO @ Sun, 21 Jun 2020 20:08:54: 24000000 INFO @ Sun, 21 Jun 2020 20:08:56: 10000000 INFO @ Sun, 21 Jun 2020 20:08:58: 25000000 INFO @ Sun, 21 Jun 2020 20:08:59: 16000000 INFO @ Sun, 21 Jun 2020 20:09:02: 11000000 INFO @ Sun, 21 Jun 2020 20:09:03: 26000000 INFO @ Sun, 21 Jun 2020 20:09:04: 17000000 INFO @ Sun, 21 Jun 2020 20:09:08: 12000000 INFO @ Sun, 21 Jun 2020 20:09:08: 27000000 INFO @ Sun, 21 Jun 2020 20:09:10: 18000000 INFO @ Sun, 21 Jun 2020 20:09:13: 28000000 INFO @ Sun, 21 Jun 2020 20:09:13: 13000000 INFO @ Sun, 21 Jun 2020 20:09:16: 19000000 INFO @ Sun, 21 Jun 2020 20:09:18: 29000000 INFO @ Sun, 21 Jun 2020 20:09:19: 14000000 INFO @ Sun, 21 Jun 2020 20:09:22: 20000000 INFO @ Sun, 21 Jun 2020 20:09:23: 30000000 INFO @ Sun, 21 Jun 2020 20:09:26: 15000000 INFO @ Sun, 21 Jun 2020 20:09:27: 21000000 INFO @ Sun, 21 Jun 2020 20:09:29: 31000000 INFO @ Sun, 21 Jun 2020 20:09:32: 16000000 INFO @ Sun, 21 Jun 2020 20:09:33: 22000000 INFO @ Sun, 21 Jun 2020 20:09:34: 32000000 INFO @ Sun, 21 Jun 2020 20:09:38: 17000000 INFO @ Sun, 21 Jun 2020 20:09:39: 23000000 INFO @ Sun, 21 Jun 2020 20:09:39: 33000000 INFO @ Sun, 21 Jun 2020 20:09:44: 18000000 INFO @ Sun, 21 Jun 2020 20:09:44: 34000000 INFO @ Sun, 21 Jun 2020 20:09:45: 24000000 INFO @ Sun, 21 Jun 2020 20:09:49: 35000000 INFO @ Sun, 21 Jun 2020 20:09:49: 19000000 INFO @ Sun, 21 Jun 2020 20:09:51: 25000000 INFO @ Sun, 21 Jun 2020 20:09:55: 36000000 INFO @ Sun, 21 Jun 2020 20:09:55: 20000000 INFO @ Sun, 21 Jun 2020 20:09:57: 26000000 INFO @ Sun, 21 Jun 2020 20:10:00: 37000000 INFO @ Sun, 21 Jun 2020 20:10:01: 21000000 INFO @ Sun, 21 Jun 2020 20:10:02: 27000000 INFO @ Sun, 21 Jun 2020 20:10:05: 38000000 INFO @ Sun, 21 Jun 2020 20:10:07: 22000000 INFO @ Sun, 21 Jun 2020 20:10:08: 28000000 INFO @ Sun, 21 Jun 2020 20:10:11: 39000000 INFO @ Sun, 21 Jun 2020 20:10:12: 23000000 INFO @ Sun, 21 Jun 2020 20:10:14: 29000000 INFO @ Sun, 21 Jun 2020 20:10:14: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:10:14: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:10:14: #1 total tags in treatment: 39519584 INFO @ Sun, 21 Jun 2020 20:10:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:10:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:10:15: #1 tags after filtering in treatment: 39519584 INFO @ Sun, 21 Jun 2020 20:10:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:10:15: #1 finished! INFO @ Sun, 21 Jun 2020 20:10:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:10:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:10:17: #2 number of paired peaks: 82 WARNING @ Sun, 21 Jun 2020 20:10:17: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:10:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:10:18: 24000000 INFO @ Sun, 21 Jun 2020 20:10:19: 30000000 INFO @ Sun, 21 Jun 2020 20:10:23: 25000000 INFO @ Sun, 21 Jun 2020 20:10:25: 31000000 INFO @ Sun, 21 Jun 2020 20:10:29: 26000000 INFO @ Sun, 21 Jun 2020 20:10:31: 32000000 INFO @ Sun, 21 Jun 2020 20:10:35: 27000000 INFO @ Sun, 21 Jun 2020 20:10:37: 33000000 INFO @ Sun, 21 Jun 2020 20:10:40: 28000000 INFO @ Sun, 21 Jun 2020 20:10:43: 34000000 INFO @ Sun, 21 Jun 2020 20:10:46: 29000000 INFO @ Sun, 21 Jun 2020 20:10:48: 35000000 INFO @ Sun, 21 Jun 2020 20:10:51: 30000000 INFO @ Sun, 21 Jun 2020 20:10:54: 36000000 INFO @ Sun, 21 Jun 2020 20:10:57: 31000000 INFO @ Sun, 21 Jun 2020 20:11:00: 37000000 INFO @ Sun, 21 Jun 2020 20:11:03: 32000000 INFO @ Sun, 21 Jun 2020 20:11:06: 38000000 INFO @ Sun, 21 Jun 2020 20:11:09: 33000000 INFO @ Sun, 21 Jun 2020 20:11:12: 39000000 INFO @ Sun, 21 Jun 2020 20:11:15: 34000000 INFO @ Sun, 21 Jun 2020 20:11:15: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:11:15: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:11:15: #1 total tags in treatment: 39519584 INFO @ Sun, 21 Jun 2020 20:11:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:11:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:11:16: #1 tags after filtering in treatment: 39519584 INFO @ Sun, 21 Jun 2020 20:11:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:11:16: #1 finished! INFO @ Sun, 21 Jun 2020 20:11:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:11:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:11:19: #2 number of paired peaks: 82 WARNING @ Sun, 21 Jun 2020 20:11:19: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:11:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:11:21: 35000000 INFO @ Sun, 21 Jun 2020 20:11:26: 36000000 INFO @ Sun, 21 Jun 2020 20:11:32: 37000000 INFO @ Sun, 21 Jun 2020 20:11:37: 38000000 INFO @ Sun, 21 Jun 2020 20:11:43: 39000000 INFO @ Sun, 21 Jun 2020 20:11:47: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:11:47: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:11:47: #1 total tags in treatment: 39519584 INFO @ Sun, 21 Jun 2020 20:11:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:11:48: #1 tags after filtering in treatment: 39519584 INFO @ Sun, 21 Jun 2020 20:11:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:11:48: #1 finished! INFO @ Sun, 21 Jun 2020 20:11:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:11:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:11:50: #2 number of paired peaks: 82 WARNING @ Sun, 21 Jun 2020 20:11:50: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:11:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3159467/SRX3159467.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。