Job ID = 12265059 SRX = SRX3088375 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 14083 reads; of these: 14083 (100.00%) were unpaired; of these: 6982 (49.58%) aligned 0 times 6159 (43.73%) aligned exactly 1 time 942 (6.69%) aligned >1 times 50.42% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 2301 / 7101 = 0.3240 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:06:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:06:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:06:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:06:00: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:06:00: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:06:00: #1 total tags in treatment: 4800 INFO @ Sat, 03 Apr 2021 06:06:00: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:06:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:06:00: #1 tags after filtering in treatment: 4697 INFO @ Sat, 03 Apr 2021 06:06:00: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 03 Apr 2021 06:06:00: #1 finished! INFO @ Sat, 03 Apr 2021 06:06:00: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:06:00: #2 number of paired peaks: 108 WARNING @ Sat, 03 Apr 2021 06:06:00: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Sat, 03 Apr 2021 06:06:00: start model_add_line... INFO @ Sat, 03 Apr 2021 06:06:00: start X-correlation... INFO @ Sat, 03 Apr 2021 06:06:00: end of X-cor INFO @ Sat, 03 Apr 2021 06:06:00: #2 finished! INFO @ Sat, 03 Apr 2021 06:06:00: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 06:06:00: #2 alternative fragment length(s) may be 9,54,74,98,126,162,209,228,251,281,302,324,347,362,400,420,432,467,480,523,542,574 bps INFO @ Sat, 03 Apr 2021 06:06:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.05_model.r WARNING @ Sat, 03 Apr 2021 06:06:00: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:06:00: #2 You may need to consider one of the other alternative d(s): 9,54,74,98,126,162,209,228,251,281,302,324,347,362,400,420,432,467,480,523,542,574 WARNING @ Sat, 03 Apr 2021 06:06:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:06:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:06:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:06:00: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:06:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:06:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:06:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.05_summits.bed INFO @ Sat, 03 Apr 2021 06:06:00: Done! pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:06:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:06:30: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:06:30: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:06:30: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:06:30: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:06:30: #1 total tags in treatment: 4800 INFO @ Sat, 03 Apr 2021 06:06:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:06:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:06:30: #1 tags after filtering in treatment: 4697 INFO @ Sat, 03 Apr 2021 06:06:30: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 03 Apr 2021 06:06:30: #1 finished! INFO @ Sat, 03 Apr 2021 06:06:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:06:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:06:30: #2 number of paired peaks: 108 WARNING @ Sat, 03 Apr 2021 06:06:30: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Sat, 03 Apr 2021 06:06:30: start model_add_line... INFO @ Sat, 03 Apr 2021 06:06:30: start X-correlation... INFO @ Sat, 03 Apr 2021 06:06:30: end of X-cor INFO @ Sat, 03 Apr 2021 06:06:30: #2 finished! INFO @ Sat, 03 Apr 2021 06:06:30: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 06:06:30: #2 alternative fragment length(s) may be 9,54,74,98,126,162,209,228,251,281,302,324,347,362,400,420,432,467,480,523,542,574 bps INFO @ Sat, 03 Apr 2021 06:06:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.10_model.r WARNING @ Sat, 03 Apr 2021 06:06:30: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:06:30: #2 You may need to consider one of the other alternative d(s): 9,54,74,98,126,162,209,228,251,281,302,324,347,362,400,420,432,467,480,523,542,574 WARNING @ Sat, 03 Apr 2021 06:06:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:06:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:06:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:06:30: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:06:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:06:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:06:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.10_summits.bed INFO @ Sat, 03 Apr 2021 06:06:30: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:07:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:07:00: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:07:00: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:07:00: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:07:00: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:07:00: #1 total tags in treatment: 4800 INFO @ Sat, 03 Apr 2021 06:07:00: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:07:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:07:00: #1 tags after filtering in treatment: 4697 INFO @ Sat, 03 Apr 2021 06:07:00: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 03 Apr 2021 06:07:00: #1 finished! INFO @ Sat, 03 Apr 2021 06:07:00: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:07:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:07:00: #2 number of paired peaks: 108 WARNING @ Sat, 03 Apr 2021 06:07:00: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Sat, 03 Apr 2021 06:07:00: start model_add_line... INFO @ Sat, 03 Apr 2021 06:07:00: start X-correlation... INFO @ Sat, 03 Apr 2021 06:07:00: end of X-cor INFO @ Sat, 03 Apr 2021 06:07:00: #2 finished! INFO @ Sat, 03 Apr 2021 06:07:00: #2 predicted fragment length is 9 bps INFO @ Sat, 03 Apr 2021 06:07:00: #2 alternative fragment length(s) may be 9,54,74,98,126,162,209,228,251,281,302,324,347,362,400,420,432,467,480,523,542,574 bps INFO @ Sat, 03 Apr 2021 06:07:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.20_model.r WARNING @ Sat, 03 Apr 2021 06:07:00: #2 Since the d (9) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:07:00: #2 You may need to consider one of the other alternative d(s): 9,54,74,98,126,162,209,228,251,281,302,324,347,362,400,420,432,467,480,523,542,574 WARNING @ Sat, 03 Apr 2021 06:07:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:07:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:07:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:07:00: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:07:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:07:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:07:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3088375/SRX3088375.20_summits.bed INFO @ Sat, 03 Apr 2021 06:07:00: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling