Job ID = 12265040 SRX = SRX3032253 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:51 17477777 reads; of these: 17477777 (100.00%) were unpaired; of these: 7066861 (40.43%) aligned 0 times 7665201 (43.86%) aligned exactly 1 time 2745715 (15.71%) aligned >1 times 59.57% overall alignment rate Time searching: 00:02:51 Overall time: 00:02:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 4885843 / 10410916 = 0.4693 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:10:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:10:22: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:10:22: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:10:27: 1000000 INFO @ Sat, 03 Apr 2021 06:10:32: 2000000 INFO @ Sat, 03 Apr 2021 06:10:36: 3000000 INFO @ Sat, 03 Apr 2021 06:10:41: 4000000 INFO @ Sat, 03 Apr 2021 06:10:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:10:50: #1 tag size is determined as 20 bps INFO @ Sat, 03 Apr 2021 06:10:50: #1 tag size = 20 INFO @ Sat, 03 Apr 2021 06:10:50: #1 total tags in treatment: 5525073 INFO @ Sat, 03 Apr 2021 06:10:50: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:10:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:10:50: #1 tags after filtering in treatment: 5525019 INFO @ Sat, 03 Apr 2021 06:10:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:10:50: #1 finished! INFO @ Sat, 03 Apr 2021 06:10:50: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:10:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:10:51: #2 number of paired peaks: 1750 INFO @ Sat, 03 Apr 2021 06:10:51: start model_add_line... INFO @ Sat, 03 Apr 2021 06:10:51: start X-correlation... INFO @ Sat, 03 Apr 2021 06:10:51: end of X-cor INFO @ Sat, 03 Apr 2021 06:10:51: #2 finished! INFO @ Sat, 03 Apr 2021 06:10:51: #2 predicted fragment length is 73 bps INFO @ Sat, 03 Apr 2021 06:10:51: #2 alternative fragment length(s) may be 73 bps INFO @ Sat, 03 Apr 2021 06:10:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.05_model.r INFO @ Sat, 03 Apr 2021 06:10:51: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:10:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:10:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:10:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:10:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:10:57: 1000000 INFO @ Sat, 03 Apr 2021 06:11:02: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:11:03: 2000000 INFO @ Sat, 03 Apr 2021 06:11:08: 3000000 INFO @ Sat, 03 Apr 2021 06:11:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:11:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:11:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.05_summits.bed INFO @ Sat, 03 Apr 2021 06:11:09: Done! pass1 - making usageList (269 chroms): 2 millis pass2 - checking and writing primary data (10533 records, 4 fields): 35 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:11:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:11:20: 5000000 INFO @ Sat, 03 Apr 2021 06:11:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:11:22: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:11:22: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:11:23: #1 tag size is determined as 20 bps INFO @ Sat, 03 Apr 2021 06:11:23: #1 tag size = 20 INFO @ Sat, 03 Apr 2021 06:11:23: #1 total tags in treatment: 5525073 INFO @ Sat, 03 Apr 2021 06:11:23: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:11:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:11:24: #1 tags after filtering in treatment: 5525019 INFO @ Sat, 03 Apr 2021 06:11:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:11:24: #1 finished! INFO @ Sat, 03 Apr 2021 06:11:24: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:11:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:11:24: #2 number of paired peaks: 1750 INFO @ Sat, 03 Apr 2021 06:11:24: start model_add_line... INFO @ Sat, 03 Apr 2021 06:11:24: start X-correlation... INFO @ Sat, 03 Apr 2021 06:11:24: end of X-cor INFO @ Sat, 03 Apr 2021 06:11:24: #2 finished! INFO @ Sat, 03 Apr 2021 06:11:24: #2 predicted fragment length is 73 bps INFO @ Sat, 03 Apr 2021 06:11:24: #2 alternative fragment length(s) may be 73 bps INFO @ Sat, 03 Apr 2021 06:11:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.10_model.r INFO @ Sat, 03 Apr 2021 06:11:24: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:11:24: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:11:28: 1000000 INFO @ Sat, 03 Apr 2021 06:11:33: 2000000 INFO @ Sat, 03 Apr 2021 06:11:36: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:11:38: 3000000 INFO @ Sat, 03 Apr 2021 06:11:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:11:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:11:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.10_summits.bed INFO @ Sat, 03 Apr 2021 06:11:42: Done! pass1 - making usageList (150 chroms): 2 millis pass2 - checking and writing primary data (4213 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:11:44: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:11:50: 5000000 INFO @ Sat, 03 Apr 2021 06:11:53: #1 tag size is determined as 20 bps INFO @ Sat, 03 Apr 2021 06:11:53: #1 tag size = 20 INFO @ Sat, 03 Apr 2021 06:11:53: #1 total tags in treatment: 5525073 INFO @ Sat, 03 Apr 2021 06:11:53: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:11:53: #1 tags after filtering in treatment: 5525019 INFO @ Sat, 03 Apr 2021 06:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:11:53: #1 finished! INFO @ Sat, 03 Apr 2021 06:11:53: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:11:54: #2 number of paired peaks: 1750 INFO @ Sat, 03 Apr 2021 06:11:54: start model_add_line... INFO @ Sat, 03 Apr 2021 06:11:54: start X-correlation... INFO @ Sat, 03 Apr 2021 06:11:54: end of X-cor INFO @ Sat, 03 Apr 2021 06:11:54: #2 finished! INFO @ Sat, 03 Apr 2021 06:11:54: #2 predicted fragment length is 73 bps INFO @ Sat, 03 Apr 2021 06:11:54: #2 alternative fragment length(s) may be 73 bps INFO @ Sat, 03 Apr 2021 06:11:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.20_model.r INFO @ Sat, 03 Apr 2021 06:11:54: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:11:54: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:12:05: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:12:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:12:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:12:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3032253/SRX3032253.20_summits.bed INFO @ Sat, 03 Apr 2021 06:12:12: Done! pass1 - making usageList (31 chroms): 1 millis pass2 - checking and writing primary data (645 records, 4 fields): 4 millis CompletedMACS2peakCalling