Job ID = 6455785 SRX = SRX2998860 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:14:36 prefetch.2.10.7: 1) Downloading 'SRR5820982'... 2020-06-21T10:14:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:36:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:36:44 prefetch.2.10.7: 1) 'SRR5820982' was downloaded successfully Read 96848614 spots for SRR5820982/SRR5820982.sra Written 96848614 spots for SRR5820982/SRR5820982.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:27 96848614 reads; of these: 96848614 (100.00%) were unpaired; of these: 3045705 (3.14%) aligned 0 times 66247565 (68.40%) aligned exactly 1 time 27555344 (28.45%) aligned >1 times 96.86% overall alignment rate Time searching: 00:30:27 Overall time: 00:30:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 43532045 / 93802909 = 0.4641 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:30:04: 1000000 INFO @ Sun, 21 Jun 2020 20:30:10: 2000000 INFO @ Sun, 21 Jun 2020 20:30:16: 3000000 INFO @ Sun, 21 Jun 2020 20:30:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:30:27: 5000000 INFO @ Sun, 21 Jun 2020 20:30:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:30:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:30:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:30:34: 6000000 INFO @ Sun, 21 Jun 2020 20:30:35: 1000000 INFO @ Sun, 21 Jun 2020 20:30:41: 7000000 INFO @ Sun, 21 Jun 2020 20:30:41: 2000000 INFO @ Sun, 21 Jun 2020 20:30:47: 8000000 INFO @ Sun, 21 Jun 2020 20:30:48: 3000000 INFO @ Sun, 21 Jun 2020 20:30:54: 9000000 INFO @ Sun, 21 Jun 2020 20:30:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:30:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:30:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:30:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:31:01: 10000000 INFO @ Sun, 21 Jun 2020 20:31:01: 5000000 INFO @ Sun, 21 Jun 2020 20:31:05: 1000000 INFO @ Sun, 21 Jun 2020 20:31:08: 11000000 INFO @ Sun, 21 Jun 2020 20:31:08: 6000000 INFO @ Sun, 21 Jun 2020 20:31:12: 2000000 INFO @ Sun, 21 Jun 2020 20:31:15: 12000000 INFO @ Sun, 21 Jun 2020 20:31:15: 7000000 INFO @ Sun, 21 Jun 2020 20:31:19: 3000000 INFO @ Sun, 21 Jun 2020 20:31:22: 13000000 INFO @ Sun, 21 Jun 2020 20:31:22: 8000000 INFO @ Sun, 21 Jun 2020 20:31:26: 4000000 INFO @ Sun, 21 Jun 2020 20:31:29: 14000000 INFO @ Sun, 21 Jun 2020 20:31:29: 9000000 INFO @ Sun, 21 Jun 2020 20:31:33: 5000000 INFO @ Sun, 21 Jun 2020 20:31:36: 15000000 INFO @ Sun, 21 Jun 2020 20:31:36: 10000000 INFO @ Sun, 21 Jun 2020 20:31:40: 6000000 INFO @ Sun, 21 Jun 2020 20:31:43: 16000000 INFO @ Sun, 21 Jun 2020 20:31:43: 11000000 INFO @ Sun, 21 Jun 2020 20:31:48: 7000000 INFO @ Sun, 21 Jun 2020 20:31:50: 17000000 INFO @ Sun, 21 Jun 2020 20:31:50: 12000000 INFO @ Sun, 21 Jun 2020 20:31:55: 8000000 INFO @ Sun, 21 Jun 2020 20:31:57: 13000000 INFO @ Sun, 21 Jun 2020 20:31:57: 18000000 INFO @ Sun, 21 Jun 2020 20:32:02: 9000000 INFO @ Sun, 21 Jun 2020 20:32:04: 14000000 INFO @ Sun, 21 Jun 2020 20:32:05: 19000000 INFO @ Sun, 21 Jun 2020 20:32:08: 10000000 INFO @ Sun, 21 Jun 2020 20:32:11: 15000000 INFO @ Sun, 21 Jun 2020 20:32:12: 20000000 INFO @ Sun, 21 Jun 2020 20:32:15: 11000000 INFO @ Sun, 21 Jun 2020 20:32:17: 16000000 INFO @ Sun, 21 Jun 2020 20:32:19: 21000000 INFO @ Sun, 21 Jun 2020 20:32:22: 12000000 INFO @ Sun, 21 Jun 2020 20:32:24: 17000000 INFO @ Sun, 21 Jun 2020 20:32:26: 22000000 INFO @ Sun, 21 Jun 2020 20:32:29: 13000000 INFO @ Sun, 21 Jun 2020 20:32:31: 18000000 INFO @ Sun, 21 Jun 2020 20:32:33: 23000000 INFO @ Sun, 21 Jun 2020 20:32:36: 14000000 INFO @ Sun, 21 Jun 2020 20:32:37: 19000000 INFO @ Sun, 21 Jun 2020 20:32:40: 24000000 INFO @ Sun, 21 Jun 2020 20:32:42: 15000000 INFO @ Sun, 21 Jun 2020 20:32:44: 20000000 INFO @ Sun, 21 Jun 2020 20:32:47: 25000000 INFO @ Sun, 21 Jun 2020 20:32:49: 16000000 INFO @ Sun, 21 Jun 2020 20:32:51: 21000000 INFO @ Sun, 21 Jun 2020 20:32:54: 26000000 INFO @ Sun, 21 Jun 2020 20:32:56: 17000000 INFO @ Sun, 21 Jun 2020 20:32:58: 22000000 INFO @ Sun, 21 Jun 2020 20:33:01: 27000000 INFO @ Sun, 21 Jun 2020 20:33:03: 18000000 INFO @ Sun, 21 Jun 2020 20:33:05: 23000000 INFO @ Sun, 21 Jun 2020 20:33:08: 28000000 INFO @ Sun, 21 Jun 2020 20:33:10: 19000000 INFO @ Sun, 21 Jun 2020 20:33:12: 24000000 INFO @ Sun, 21 Jun 2020 20:33:15: 29000000 INFO @ Sun, 21 Jun 2020 20:33:17: 20000000 INFO @ Sun, 21 Jun 2020 20:33:18: 25000000 INFO @ Sun, 21 Jun 2020 20:33:22: 30000000 INFO @ Sun, 21 Jun 2020 20:33:24: 21000000 INFO @ Sun, 21 Jun 2020 20:33:25: 26000000 INFO @ Sun, 21 Jun 2020 20:33:28: 31000000 INFO @ Sun, 21 Jun 2020 20:33:31: 22000000 INFO @ Sun, 21 Jun 2020 20:33:32: 27000000 INFO @ Sun, 21 Jun 2020 20:33:35: 32000000 INFO @ Sun, 21 Jun 2020 20:33:38: 23000000 INFO @ Sun, 21 Jun 2020 20:33:39: 28000000 INFO @ Sun, 21 Jun 2020 20:33:42: 33000000 INFO @ Sun, 21 Jun 2020 20:33:44: 24000000 INFO @ Sun, 21 Jun 2020 20:33:45: 29000000 INFO @ Sun, 21 Jun 2020 20:33:48: 34000000 INFO @ Sun, 21 Jun 2020 20:33:51: 25000000 INFO @ Sun, 21 Jun 2020 20:33:52: 30000000 INFO @ Sun, 21 Jun 2020 20:33:55: 35000000 INFO @ Sun, 21 Jun 2020 20:33:58: 26000000 INFO @ Sun, 21 Jun 2020 20:33:59: 31000000 INFO @ Sun, 21 Jun 2020 20:34:02: 36000000 INFO @ Sun, 21 Jun 2020 20:34:05: 27000000 INFO @ Sun, 21 Jun 2020 20:34:06: 32000000 INFO @ Sun, 21 Jun 2020 20:34:09: 37000000 INFO @ Sun, 21 Jun 2020 20:34:11: 28000000 INFO @ Sun, 21 Jun 2020 20:34:13: 33000000 INFO @ Sun, 21 Jun 2020 20:34:16: 38000000 INFO @ Sun, 21 Jun 2020 20:34:18: 29000000 INFO @ Sun, 21 Jun 2020 20:34:20: 34000000 INFO @ Sun, 21 Jun 2020 20:34:23: 39000000 INFO @ Sun, 21 Jun 2020 20:34:25: 30000000 INFO @ Sun, 21 Jun 2020 20:34:27: 35000000 INFO @ Sun, 21 Jun 2020 20:34:30: 40000000 INFO @ Sun, 21 Jun 2020 20:34:32: 31000000 INFO @ Sun, 21 Jun 2020 20:34:33: 36000000 INFO @ Sun, 21 Jun 2020 20:34:37: 41000000 INFO @ Sun, 21 Jun 2020 20:34:39: 32000000 INFO @ Sun, 21 Jun 2020 20:34:40: 37000000 INFO @ Sun, 21 Jun 2020 20:34:44: 42000000 INFO @ Sun, 21 Jun 2020 20:34:46: 33000000 INFO @ Sun, 21 Jun 2020 20:34:47: 38000000 INFO @ Sun, 21 Jun 2020 20:34:51: 43000000 INFO @ Sun, 21 Jun 2020 20:34:52: 34000000 INFO @ Sun, 21 Jun 2020 20:34:54: 39000000 INFO @ Sun, 21 Jun 2020 20:34:58: 44000000 INFO @ Sun, 21 Jun 2020 20:34:59: 35000000 INFO @ Sun, 21 Jun 2020 20:35:01: 40000000 INFO @ Sun, 21 Jun 2020 20:35:04: 45000000 INFO @ Sun, 21 Jun 2020 20:35:06: 36000000 INFO @ Sun, 21 Jun 2020 20:35:08: 41000000 INFO @ Sun, 21 Jun 2020 20:35:11: 46000000 INFO @ Sun, 21 Jun 2020 20:35:13: 37000000 BedGraph に変換しました。 INFO @ Sun, 21 Jun 2020 20:35:15: 42000000 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:35:18: 47000000 INFO @ Sun, 21 Jun 2020 20:35:20: 38000000 INFO @ Sun, 21 Jun 2020 20:35:22: 43000000 INFO @ Sun, 21 Jun 2020 20:35:25: 48000000 INFO @ Sun, 21 Jun 2020 20:35:27: 39000000 INFO @ Sun, 21 Jun 2020 20:35:29: 44000000 INFO @ Sun, 21 Jun 2020 20:35:32: 49000000 INFO @ Sun, 21 Jun 2020 20:35:34: 40000000 INFO @ Sun, 21 Jun 2020 20:35:36: 45000000 INFO @ Sun, 21 Jun 2020 20:35:39: 50000000 INFO @ Sun, 21 Jun 2020 20:35:41: 41000000 INFO @ Sun, 21 Jun 2020 20:35:41: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 20:35:41: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 20:35:41: #1 total tags in treatment: 50270864 INFO @ Sun, 21 Jun 2020 20:35:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:35:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:35:42: 46000000 INFO @ Sun, 21 Jun 2020 20:35:43: #1 tags after filtering in treatment: 50270864 INFO @ Sun, 21 Jun 2020 20:35:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:35:43: #1 finished! INFO @ Sun, 21 Jun 2020 20:35:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:35:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:35:46: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:35:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:35:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:35:48: 42000000 INFO @ Sun, 21 Jun 2020 20:35:49: 47000000 INFO @ Sun, 21 Jun 2020 20:35:55: 43000000 INFO @ Sun, 21 Jun 2020 20:35:56: 48000000 INFO @ Sun, 21 Jun 2020 20:36:02: 44000000 INFO @ Sun, 21 Jun 2020 20:36:03: 49000000 INFO @ Sun, 21 Jun 2020 20:36:09: 45000000 INFO @ Sun, 21 Jun 2020 20:36:10: 50000000 INFO @ Sun, 21 Jun 2020 20:36:12: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 20:36:12: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 20:36:12: #1 total tags in treatment: 50270864 INFO @ Sun, 21 Jun 2020 20:36:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:36:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:36:14: #1 tags after filtering in treatment: 50270864 INFO @ Sun, 21 Jun 2020 20:36:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:36:14: #1 finished! INFO @ Sun, 21 Jun 2020 20:36:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:36:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:36:16: 46000000 INFO @ Sun, 21 Jun 2020 20:36:17: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:36:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:36:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:36:22: 47000000 INFO @ Sun, 21 Jun 2020 20:36:29: 48000000 INFO @ Sun, 21 Jun 2020 20:36:36: 49000000 INFO @ Sun, 21 Jun 2020 20:36:42: 50000000 INFO @ Sun, 21 Jun 2020 20:36:44: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 20:36:44: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 20:36:44: #1 total tags in treatment: 50270864 INFO @ Sun, 21 Jun 2020 20:36:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:36:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:36:45: #1 tags after filtering in treatment: 50270864 INFO @ Sun, 21 Jun 2020 20:36:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:36:45: #1 finished! INFO @ Sun, 21 Jun 2020 20:36:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:36:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:36:48: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:36:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:36:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2998860/SRX2998860.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。