Job ID = 6455678 SRX = SRX287982 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:16:36 prefetch.2.10.7: 1) Downloading 'SRR870171'... 2020-06-21T10:16:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:20:27 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:20:28 prefetch.2.10.7: 'SRR870171' is valid 2020-06-21T10:20:28 prefetch.2.10.7: 1) 'SRR870171' was downloaded successfully Read 14061078 spots for SRR870171/SRR870171.sra Written 14061078 spots for SRR870171/SRR870171.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:55 14061078 reads; of these: 14061078 (100.00%) were unpaired; of these: 1145604 (8.15%) aligned 0 times 11358146 (80.78%) aligned exactly 1 time 1557328 (11.08%) aligned >1 times 91.85% overall alignment rate Time searching: 00:02:55 Overall time: 00:02:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2763758 / 12915474 = 0.2140 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:27:35: 1000000 INFO @ Sun, 21 Jun 2020 19:27:40: 2000000 INFO @ Sun, 21 Jun 2020 19:27:45: 3000000 INFO @ Sun, 21 Jun 2020 19:27:50: 4000000 INFO @ Sun, 21 Jun 2020 19:27:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:28:00: 6000000 INFO @ Sun, 21 Jun 2020 19:28:05: 7000000 INFO @ Sun, 21 Jun 2020 19:28:06: 1000000 INFO @ Sun, 21 Jun 2020 19:28:11: 8000000 INFO @ Sun, 21 Jun 2020 19:28:12: 2000000 INFO @ Sun, 21 Jun 2020 19:28:17: 9000000 INFO @ Sun, 21 Jun 2020 19:28:19: 3000000 INFO @ Sun, 21 Jun 2020 19:28:23: 10000000 INFO @ Sun, 21 Jun 2020 19:28:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:28:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:28:24: #1 total tags in treatment: 10151716 INFO @ Sun, 21 Jun 2020 19:28:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:28:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:28:24: #1 tags after filtering in treatment: 10151702 INFO @ Sun, 21 Jun 2020 19:28:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:28:24: #1 finished! INFO @ Sun, 21 Jun 2020 19:28:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:28:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:28:25: 4000000 INFO @ Sun, 21 Jun 2020 19:28:25: #2 number of paired peaks: 66 WARNING @ Sun, 21 Jun 2020 19:28:25: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:28:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:28:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:28:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:28:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:28:31: 5000000 INFO @ Sun, 21 Jun 2020 19:28:36: 1000000 INFO @ Sun, 21 Jun 2020 19:28:37: 6000000 INFO @ Sun, 21 Jun 2020 19:28:43: 2000000 INFO @ Sun, 21 Jun 2020 19:28:44: 7000000 INFO @ Sun, 21 Jun 2020 19:28:49: 3000000 INFO @ Sun, 21 Jun 2020 19:28:50: 8000000 INFO @ Sun, 21 Jun 2020 19:28:56: 4000000 INFO @ Sun, 21 Jun 2020 19:28:57: 9000000 INFO @ Sun, 21 Jun 2020 19:29:02: 5000000 INFO @ Sun, 21 Jun 2020 19:29:04: 10000000 INFO @ Sun, 21 Jun 2020 19:29:05: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:29:05: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:29:05: #1 total tags in treatment: 10151716 INFO @ Sun, 21 Jun 2020 19:29:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:29:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:29:05: #1 tags after filtering in treatment: 10151702 INFO @ Sun, 21 Jun 2020 19:29:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:29:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:29:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:29:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:29:06: #2 number of paired peaks: 66 WARNING @ Sun, 21 Jun 2020 19:29:06: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:29:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:29:08: 6000000 INFO @ Sun, 21 Jun 2020 19:29:14: 7000000 INFO @ Sun, 21 Jun 2020 19:29:21: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:29:27: 9000000 INFO @ Sun, 21 Jun 2020 19:29:34: 10000000 INFO @ Sun, 21 Jun 2020 19:29:35: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:29:35: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:29:35: #1 total tags in treatment: 10151716 INFO @ Sun, 21 Jun 2020 19:29:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:29:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:29:35: #1 tags after filtering in treatment: 10151702 INFO @ Sun, 21 Jun 2020 19:29:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:29:35: #1 finished! INFO @ Sun, 21 Jun 2020 19:29:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:29:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:29:36: #2 number of paired peaks: 66 WARNING @ Sun, 21 Jun 2020 19:29:36: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:29:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287982/SRX287982.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。