Job ID = 6455665 SRX = SRX287970 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:17:51 prefetch.2.10.7: 1) Downloading 'SRR870159'... 2020-06-21T10:17:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:20:32 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:20:33 prefetch.2.10.7: 'SRR870159' is valid 2020-06-21T10:20:33 prefetch.2.10.7: 1) 'SRR870159' was downloaded successfully Read 11846426 spots for SRR870159/SRR870159.sra Written 11846426 spots for SRR870159/SRR870159.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:44 11846426 reads; of these: 11846426 (100.00%) were unpaired; of these: 666468 (5.63%) aligned 0 times 9758001 (82.37%) aligned exactly 1 time 1421957 (12.00%) aligned >1 times 94.37% overall alignment rate Time searching: 00:02:44 Overall time: 00:02:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 979962 / 11179958 = 0.0877 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:27:16: 1000000 INFO @ Sun, 21 Jun 2020 19:27:23: 2000000 INFO @ Sun, 21 Jun 2020 19:27:31: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:27:39: 4000000 INFO @ Sun, 21 Jun 2020 19:27:46: 1000000 INFO @ Sun, 21 Jun 2020 19:27:48: 5000000 INFO @ Sun, 21 Jun 2020 19:27:54: 2000000 INFO @ Sun, 21 Jun 2020 19:27:58: 6000000 INFO @ Sun, 21 Jun 2020 19:28:02: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:28:06: 7000000 INFO @ Sun, 21 Jun 2020 19:28:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:28:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:28:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:28:10: 4000000 INFO @ Sun, 21 Jun 2020 19:28:16: 8000000 INFO @ Sun, 21 Jun 2020 19:28:16: 1000000 INFO @ Sun, 21 Jun 2020 19:28:19: 5000000 INFO @ Sun, 21 Jun 2020 19:28:25: 2000000 INFO @ Sun, 21 Jun 2020 19:28:26: 9000000 INFO @ Sun, 21 Jun 2020 19:28:28: 6000000 INFO @ Sun, 21 Jun 2020 19:28:34: 3000000 INFO @ Sun, 21 Jun 2020 19:28:36: 10000000 INFO @ Sun, 21 Jun 2020 19:28:36: 7000000 INFO @ Sun, 21 Jun 2020 19:28:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:28:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:28:38: #1 total tags in treatment: 10199996 INFO @ Sun, 21 Jun 2020 19:28:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:28:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:28:39: #1 tags after filtering in treatment: 10199983 INFO @ Sun, 21 Jun 2020 19:28:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:28:39: #1 finished! INFO @ Sun, 21 Jun 2020 19:28:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:28:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:28:40: #2 number of paired peaks: 37 WARNING @ Sun, 21 Jun 2020 19:28:40: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:28:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:28:42: 4000000 INFO @ Sun, 21 Jun 2020 19:28:44: 8000000 INFO @ Sun, 21 Jun 2020 19:28:50: 5000000 INFO @ Sun, 21 Jun 2020 19:28:53: 9000000 INFO @ Sun, 21 Jun 2020 19:28:58: 6000000 INFO @ Sun, 21 Jun 2020 19:29:00: 10000000 INFO @ Sun, 21 Jun 2020 19:29:02: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:29:02: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:29:02: #1 total tags in treatment: 10199996 INFO @ Sun, 21 Jun 2020 19:29:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:29:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:29:03: #1 tags after filtering in treatment: 10199983 INFO @ Sun, 21 Jun 2020 19:29:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:29:03: #1 finished! INFO @ Sun, 21 Jun 2020 19:29:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:29:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:29:03: #2 number of paired peaks: 37 WARNING @ Sun, 21 Jun 2020 19:29:03: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:29:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:29:06: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:29:13: 8000000 INFO @ Sun, 21 Jun 2020 19:29:20: 9000000 INFO @ Sun, 21 Jun 2020 19:29:28: 10000000 INFO @ Sun, 21 Jun 2020 19:29:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:29:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:29:29: #1 total tags in treatment: 10199996 INFO @ Sun, 21 Jun 2020 19:29:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:29:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:29:30: #1 tags after filtering in treatment: 10199983 INFO @ Sun, 21 Jun 2020 19:29:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:29:30: #1 finished! INFO @ Sun, 21 Jun 2020 19:29:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:29:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:29:30: #2 number of paired peaks: 37 WARNING @ Sun, 21 Jun 2020 19:29:30: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:29:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287970/SRX287970.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。