Job ID = 6455664 SRX = SRX287969 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:32:35 prefetch.2.10.7: 1) Downloading 'SRR870158'... 2020-06-21T10:32:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:34:58 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:34:58 prefetch.2.10.7: 'SRR870158' is valid 2020-06-21T10:34:58 prefetch.2.10.7: 1) 'SRR870158' was downloaded successfully Read 12539599 spots for SRR870158/SRR870158.sra Written 12539599 spots for SRR870158/SRR870158.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:00 12539599 reads; of these: 12539599 (100.00%) were unpaired; of these: 668420 (5.33%) aligned 0 times 10381083 (82.79%) aligned exactly 1 time 1490096 (11.88%) aligned >1 times 94.67% overall alignment rate Time searching: 00:03:00 Overall time: 00:03:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1086746 / 11871179 = 0.0915 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:43:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:43:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:43:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:43:09: 1000000 INFO @ Sun, 21 Jun 2020 19:43:17: 2000000 INFO @ Sun, 21 Jun 2020 19:43:25: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:43:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:43:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:43:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:43:32: 4000000 INFO @ Sun, 21 Jun 2020 19:43:40: 1000000 INFO @ Sun, 21 Jun 2020 19:43:41: 5000000 INFO @ Sun, 21 Jun 2020 19:43:48: 2000000 INFO @ Sun, 21 Jun 2020 19:43:50: 6000000 INFO @ Sun, 21 Jun 2020 19:43:57: 3000000 INFO @ Sun, 21 Jun 2020 19:43:58: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:44:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:44:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:44:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:44:06: 4000000 INFO @ Sun, 21 Jun 2020 19:44:07: 8000000 INFO @ Sun, 21 Jun 2020 19:44:10: 1000000 INFO @ Sun, 21 Jun 2020 19:44:15: 5000000 INFO @ Sun, 21 Jun 2020 19:44:16: 9000000 INFO @ Sun, 21 Jun 2020 19:44:19: 2000000 INFO @ Sun, 21 Jun 2020 19:44:24: 6000000 INFO @ Sun, 21 Jun 2020 19:44:26: 10000000 INFO @ Sun, 21 Jun 2020 19:44:28: 3000000 INFO @ Sun, 21 Jun 2020 19:44:33: 7000000 INFO @ Sun, 21 Jun 2020 19:44:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:44:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:44:33: #1 total tags in treatment: 10784433 INFO @ Sun, 21 Jun 2020 19:44:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:44:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:44:34: #1 tags after filtering in treatment: 10784423 INFO @ Sun, 21 Jun 2020 19:44:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:44:34: #1 finished! INFO @ Sun, 21 Jun 2020 19:44:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:44:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:44:35: #2 number of paired peaks: 58 WARNING @ Sun, 21 Jun 2020 19:44:35: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:44:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:44:37: 4000000 INFO @ Sun, 21 Jun 2020 19:44:42: 8000000 INFO @ Sun, 21 Jun 2020 19:44:45: 5000000 INFO @ Sun, 21 Jun 2020 19:44:50: 9000000 INFO @ Sun, 21 Jun 2020 19:44:54: 6000000 INFO @ Sun, 21 Jun 2020 19:45:00: 10000000 INFO @ Sun, 21 Jun 2020 19:45:02: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:45:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:45:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:45:07: #1 total tags in treatment: 10784433 INFO @ Sun, 21 Jun 2020 19:45:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:45:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:45:08: #1 tags after filtering in treatment: 10784423 INFO @ Sun, 21 Jun 2020 19:45:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:45:08: #1 finished! INFO @ Sun, 21 Jun 2020 19:45:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:45:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:45:08: #2 number of paired peaks: 58 WARNING @ Sun, 21 Jun 2020 19:45:08: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:45:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:45:10: 8000000 INFO @ Sun, 21 Jun 2020 19:45:18: 9000000 INFO @ Sun, 21 Jun 2020 19:45:27: 10000000 INFO @ Sun, 21 Jun 2020 19:45:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:45:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:45:34: #1 total tags in treatment: 10784433 INFO @ Sun, 21 Jun 2020 19:45:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:45:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:45:34: #1 tags after filtering in treatment: 10784423 INFO @ Sun, 21 Jun 2020 19:45:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:45:34: #1 finished! INFO @ Sun, 21 Jun 2020 19:45:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:45:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:45:35: #2 number of paired peaks: 58 WARNING @ Sun, 21 Jun 2020 19:45:35: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:45:35: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287969/SRX287969.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling