Job ID = 6455526 SRX = SRX287856 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:58:55 prefetch.2.10.7: 1) Downloading 'SRR870045'... 2020-06-21T09:58:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:01:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:01:56 prefetch.2.10.7: 1) 'SRR870045' was downloaded successfully Read 18092877 spots for SRR870045/SRR870045.sra Written 18092877 spots for SRR870045/SRR870045.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:10 18092877 reads; of these: 18092877 (100.00%) were unpaired; of these: 1321438 (7.30%) aligned 0 times 14793753 (81.77%) aligned exactly 1 time 1977686 (10.93%) aligned >1 times 92.70% overall alignment rate Time searching: 00:04:10 Overall time: 00:04:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1681453 / 16771439 = 0.1003 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:11:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:11:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:11:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:11:29: 1000000 INFO @ Sun, 21 Jun 2020 19:11:35: 2000000 INFO @ Sun, 21 Jun 2020 19:11:40: 3000000 INFO @ Sun, 21 Jun 2020 19:11:46: 4000000 INFO @ Sun, 21 Jun 2020 19:11:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:11:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:11:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:11:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:11:57: 6000000 INFO @ Sun, 21 Jun 2020 19:12:00: 1000000 INFO @ Sun, 21 Jun 2020 19:12:04: 7000000 INFO @ Sun, 21 Jun 2020 19:12:06: 2000000 INFO @ Sun, 21 Jun 2020 19:12:10: 8000000 INFO @ Sun, 21 Jun 2020 19:12:12: 3000000 INFO @ Sun, 21 Jun 2020 19:12:17: 9000000 INFO @ Sun, 21 Jun 2020 19:12:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:12:23: 10000000 INFO @ Sun, 21 Jun 2020 19:12:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:12:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:12:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:12:25: 5000000 INFO @ Sun, 21 Jun 2020 19:12:30: 11000000 INFO @ Sun, 21 Jun 2020 19:12:30: 1000000 INFO @ Sun, 21 Jun 2020 19:12:32: 6000000 INFO @ Sun, 21 Jun 2020 19:12:36: 12000000 INFO @ Sun, 21 Jun 2020 19:12:38: 2000000 INFO @ Sun, 21 Jun 2020 19:12:38: 7000000 INFO @ Sun, 21 Jun 2020 19:12:43: 13000000 INFO @ Sun, 21 Jun 2020 19:12:45: 8000000 INFO @ Sun, 21 Jun 2020 19:12:45: 3000000 INFO @ Sun, 21 Jun 2020 19:12:50: 14000000 INFO @ Sun, 21 Jun 2020 19:12:51: 9000000 INFO @ Sun, 21 Jun 2020 19:12:52: 4000000 INFO @ Sun, 21 Jun 2020 19:12:56: 15000000 INFO @ Sun, 21 Jun 2020 19:12:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:12:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:12:57: #1 total tags in treatment: 15089986 INFO @ Sun, 21 Jun 2020 19:12:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:12:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:12:58: #1 tags after filtering in treatment: 15089948 INFO @ Sun, 21 Jun 2020 19:12:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:12:58: #1 finished! INFO @ Sun, 21 Jun 2020 19:12:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:12:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:12:58: 10000000 INFO @ Sun, 21 Jun 2020 19:12:59: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 19:12:59: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:12:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:13:00: 5000000 INFO @ Sun, 21 Jun 2020 19:13:04: 11000000 INFO @ Sun, 21 Jun 2020 19:13:07: 6000000 INFO @ Sun, 21 Jun 2020 19:13:11: 12000000 INFO @ Sun, 21 Jun 2020 19:13:14: 7000000 INFO @ Sun, 21 Jun 2020 19:13:18: 13000000 INFO @ Sun, 21 Jun 2020 19:13:21: 8000000 INFO @ Sun, 21 Jun 2020 19:13:25: 14000000 INFO @ Sun, 21 Jun 2020 19:13:29: 9000000 INFO @ Sun, 21 Jun 2020 19:13:32: 15000000 INFO @ Sun, 21 Jun 2020 19:13:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:13:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:13:33: #1 total tags in treatment: 15089986 INFO @ Sun, 21 Jun 2020 19:13:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:13:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:13:33: #1 tags after filtering in treatment: 15089948 INFO @ Sun, 21 Jun 2020 19:13:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:13:33: #1 finished! INFO @ Sun, 21 Jun 2020 19:13:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:13:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:13:34: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 19:13:34: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:13:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:13:36: 10000000 INFO @ Sun, 21 Jun 2020 19:13:42: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:13:49: 12000000 INFO @ Sun, 21 Jun 2020 19:13:56: 13000000 INFO @ Sun, 21 Jun 2020 19:14:02: 14000000 INFO @ Sun, 21 Jun 2020 19:14:09: 15000000 INFO @ Sun, 21 Jun 2020 19:14:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:14:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:14:10: #1 total tags in treatment: 15089986 INFO @ Sun, 21 Jun 2020 19:14:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:14:10: #1 tags after filtering in treatment: 15089948 INFO @ Sun, 21 Jun 2020 19:14:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:14:10: #1 finished! INFO @ Sun, 21 Jun 2020 19:14:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:14:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:14:11: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 19:14:11: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:14:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287856/SRX287856.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。