Job ID = 6455525 SRX = SRX287855 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:17:06 prefetch.2.10.7: 1) Downloading 'SRR870044'... 2020-06-21T10:17:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:20:35 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:20:35 prefetch.2.10.7: 1) 'SRR870044' was downloaded successfully Read 18586853 spots for SRR870044/SRR870044.sra Written 18586853 spots for SRR870044/SRR870044.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:05 18586853 reads; of these: 18586853 (100.00%) were unpaired; of these: 1415064 (7.61%) aligned 0 times 15247386 (82.03%) aligned exactly 1 time 1924403 (10.35%) aligned >1 times 92.39% overall alignment rate Time searching: 00:04:05 Overall time: 00:04:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1875657 / 17171789 = 0.1092 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:30:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:30:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:30:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:30:10: 1000000 INFO @ Sun, 21 Jun 2020 19:30:15: 2000000 INFO @ Sun, 21 Jun 2020 19:30:19: 3000000 INFO @ Sun, 21 Jun 2020 19:30:24: 4000000 INFO @ Sun, 21 Jun 2020 19:30:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:30:34: 6000000 INFO @ Sun, 21 Jun 2020 19:30:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:30:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:30:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:30:39: 7000000 INFO @ Sun, 21 Jun 2020 19:30:40: 1000000 INFO @ Sun, 21 Jun 2020 19:30:44: 8000000 INFO @ Sun, 21 Jun 2020 19:30:45: 2000000 INFO @ Sun, 21 Jun 2020 19:30:49: 9000000 INFO @ Sun, 21 Jun 2020 19:30:50: 3000000 INFO @ Sun, 21 Jun 2020 19:30:54: 10000000 INFO @ Sun, 21 Jun 2020 19:30:55: 4000000 INFO @ Sun, 21 Jun 2020 19:30:59: 11000000 INFO @ Sun, 21 Jun 2020 19:31:00: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:31:04: 12000000 INFO @ Sun, 21 Jun 2020 19:31:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:31:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:31:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:31:05: 6000000 INFO @ Sun, 21 Jun 2020 19:31:09: 13000000 INFO @ Sun, 21 Jun 2020 19:31:10: 7000000 INFO @ Sun, 21 Jun 2020 19:31:10: 1000000 INFO @ Sun, 21 Jun 2020 19:31:15: 14000000 INFO @ Sun, 21 Jun 2020 19:31:15: 8000000 INFO @ Sun, 21 Jun 2020 19:31:15: 2000000 INFO @ Sun, 21 Jun 2020 19:31:20: 15000000 INFO @ Sun, 21 Jun 2020 19:31:20: 9000000 INFO @ Sun, 21 Jun 2020 19:31:20: 3000000 INFO @ Sun, 21 Jun 2020 19:31:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:31:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:31:22: #1 total tags in treatment: 15296132 INFO @ Sun, 21 Jun 2020 19:31:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:31:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:31:22: #1 tags after filtering in treatment: 15296074 INFO @ Sun, 21 Jun 2020 19:31:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:31:22: #1 finished! INFO @ Sun, 21 Jun 2020 19:31:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:31:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:31:23: #2 number of paired peaks: 90 WARNING @ Sun, 21 Jun 2020 19:31:23: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:31:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:31:25: 10000000 INFO @ Sun, 21 Jun 2020 19:31:25: 4000000 INFO @ Sun, 21 Jun 2020 19:31:30: 11000000 INFO @ Sun, 21 Jun 2020 19:31:30: 5000000 INFO @ Sun, 21 Jun 2020 19:31:35: 12000000 INFO @ Sun, 21 Jun 2020 19:31:35: 6000000 INFO @ Sun, 21 Jun 2020 19:31:40: 13000000 INFO @ Sun, 21 Jun 2020 19:31:40: 7000000 INFO @ Sun, 21 Jun 2020 19:31:45: 8000000 INFO @ Sun, 21 Jun 2020 19:31:45: 14000000 INFO @ Sun, 21 Jun 2020 19:31:50: 9000000 INFO @ Sun, 21 Jun 2020 19:31:51: 15000000 INFO @ Sun, 21 Jun 2020 19:31:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:31:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:31:52: #1 total tags in treatment: 15296132 INFO @ Sun, 21 Jun 2020 19:31:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:31:53: #1 tags after filtering in treatment: 15296074 INFO @ Sun, 21 Jun 2020 19:31:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:31:53: #1 finished! INFO @ Sun, 21 Jun 2020 19:31:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:31:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:31:54: #2 number of paired peaks: 90 WARNING @ Sun, 21 Jun 2020 19:31:54: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:31:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:31:55: 10000000 INFO @ Sun, 21 Jun 2020 19:32:00: 11000000 INFO @ Sun, 21 Jun 2020 19:32:05: 12000000 INFO @ Sun, 21 Jun 2020 19:32:10: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:32:15: 14000000 INFO @ Sun, 21 Jun 2020 19:32:20: 15000000 INFO @ Sun, 21 Jun 2020 19:32:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:32:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:32:22: #1 total tags in treatment: 15296132 INFO @ Sun, 21 Jun 2020 19:32:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:32:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:32:22: #1 tags after filtering in treatment: 15296074 INFO @ Sun, 21 Jun 2020 19:32:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:32:22: #1 finished! INFO @ Sun, 21 Jun 2020 19:32:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:32:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:32:23: #2 number of paired peaks: 90 WARNING @ Sun, 21 Jun 2020 19:32:23: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:32:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287855/SRX287855.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。